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In Peripheral Blood Mononuclear Cells Helicobacter pylori Induces the Secretion of Soluble and Exosomal Cytokines Related to Carcinogenesis

Helicobacter pylori promotes the secretion of cytokines that regulate inflammation and carcinogenesis. Immune cells secrete cytokines into the extracellular medium or packaged in exosomes. The objective of this study was to analyze the profile of soluble and exosomal cytokines that were secreted by...

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Autores principales: Atrisco-Morales, Josefina, Ramírez, Mónica, Castañón-Sánchez, Carlos Alberto, Román-Román, Adolfo, Román-Fernández, Ilce Valeria, Martínez-Carrillo, Dinorah Nashely, García-Arellano, Samuel, Muñoz-Valle, José Francisco, Rodríguez-Ruiz, Hugo Alberto, Fernández-Tilapa, Gloria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9368997/
https://www.ncbi.nlm.nih.gov/pubmed/35955936
http://dx.doi.org/10.3390/ijms23158801
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author Atrisco-Morales, Josefina
Ramírez, Mónica
Castañón-Sánchez, Carlos Alberto
Román-Román, Adolfo
Román-Fernández, Ilce Valeria
Martínez-Carrillo, Dinorah Nashely
García-Arellano, Samuel
Muñoz-Valle, José Francisco
Rodríguez-Ruiz, Hugo Alberto
Fernández-Tilapa, Gloria
author_facet Atrisco-Morales, Josefina
Ramírez, Mónica
Castañón-Sánchez, Carlos Alberto
Román-Román, Adolfo
Román-Fernández, Ilce Valeria
Martínez-Carrillo, Dinorah Nashely
García-Arellano, Samuel
Muñoz-Valle, José Francisco
Rodríguez-Ruiz, Hugo Alberto
Fernández-Tilapa, Gloria
author_sort Atrisco-Morales, Josefina
collection PubMed
description Helicobacter pylori promotes the secretion of cytokines that regulate inflammation and carcinogenesis. Immune cells secrete cytokines into the extracellular medium or packaged in exosomes. The objective of this study was to analyze the profile of soluble and exosomal cytokines that were secreted by human peripheral blood mononuclear cells (PBMCs) that were infected with H. pylori and to build a network of interaction between cytokines and cellular proteins. PBMCs were obtained by density gradient centrifugation and infected with H. pylori for 24 h. The infection was verified by immunofluorescence and Western blot for CagA. The exosomes were obtained from culture supernatant by ultracentrifugation and characterized by transmission electron microscopy, particle size analysis, and Western blot for CD9 and CD81. Cytokines were quantified using a multiplex immunoassay in the culture supernatant, intact exosomes, and lysed exosomes. H. pylori adheres to lymphocytes and translocates CagA. In PBMCs, H. pylori induces an increase in the soluble and exosomal IL-1β, IL-6, TNF-α, IL-10, IL-17A, IL-21, and IL-22. The protein–protein interaction (PPI) network shows that soluble and exosomal cytokines interact with proteins that participate in signaling pathways such as NF-κB, MAPK, PI3K-Akt, Jak-STAT, FoxO, and mTOR, that are related to carcinogenesis; moreover, TNF-α had the highest number of interactions. Cytokine-loaded exosomes represent another means of intercellular communication that is activated by H. pylori to stimulate inflammation, carcinogenesis, or cancer progression. Cytokine-loaded exosomes are likely to be associated with extragastrointestinal diseases of inflammatory origin.
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spelling pubmed-93689972022-08-12 In Peripheral Blood Mononuclear Cells Helicobacter pylori Induces the Secretion of Soluble and Exosomal Cytokines Related to Carcinogenesis Atrisco-Morales, Josefina Ramírez, Mónica Castañón-Sánchez, Carlos Alberto Román-Román, Adolfo Román-Fernández, Ilce Valeria Martínez-Carrillo, Dinorah Nashely García-Arellano, Samuel Muñoz-Valle, José Francisco Rodríguez-Ruiz, Hugo Alberto Fernández-Tilapa, Gloria Int J Mol Sci Article Helicobacter pylori promotes the secretion of cytokines that regulate inflammation and carcinogenesis. Immune cells secrete cytokines into the extracellular medium or packaged in exosomes. The objective of this study was to analyze the profile of soluble and exosomal cytokines that were secreted by human peripheral blood mononuclear cells (PBMCs) that were infected with H. pylori and to build a network of interaction between cytokines and cellular proteins. PBMCs were obtained by density gradient centrifugation and infected with H. pylori for 24 h. The infection was verified by immunofluorescence and Western blot for CagA. The exosomes were obtained from culture supernatant by ultracentrifugation and characterized by transmission electron microscopy, particle size analysis, and Western blot for CD9 and CD81. Cytokines were quantified using a multiplex immunoassay in the culture supernatant, intact exosomes, and lysed exosomes. H. pylori adheres to lymphocytes and translocates CagA. In PBMCs, H. pylori induces an increase in the soluble and exosomal IL-1β, IL-6, TNF-α, IL-10, IL-17A, IL-21, and IL-22. The protein–protein interaction (PPI) network shows that soluble and exosomal cytokines interact with proteins that participate in signaling pathways such as NF-κB, MAPK, PI3K-Akt, Jak-STAT, FoxO, and mTOR, that are related to carcinogenesis; moreover, TNF-α had the highest number of interactions. Cytokine-loaded exosomes represent another means of intercellular communication that is activated by H. pylori to stimulate inflammation, carcinogenesis, or cancer progression. Cytokine-loaded exosomes are likely to be associated with extragastrointestinal diseases of inflammatory origin. MDPI 2022-08-08 /pmc/articles/PMC9368997/ /pubmed/35955936 http://dx.doi.org/10.3390/ijms23158801 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Atrisco-Morales, Josefina
Ramírez, Mónica
Castañón-Sánchez, Carlos Alberto
Román-Román, Adolfo
Román-Fernández, Ilce Valeria
Martínez-Carrillo, Dinorah Nashely
García-Arellano, Samuel
Muñoz-Valle, José Francisco
Rodríguez-Ruiz, Hugo Alberto
Fernández-Tilapa, Gloria
In Peripheral Blood Mononuclear Cells Helicobacter pylori Induces the Secretion of Soluble and Exosomal Cytokines Related to Carcinogenesis
title In Peripheral Blood Mononuclear Cells Helicobacter pylori Induces the Secretion of Soluble and Exosomal Cytokines Related to Carcinogenesis
title_full In Peripheral Blood Mononuclear Cells Helicobacter pylori Induces the Secretion of Soluble and Exosomal Cytokines Related to Carcinogenesis
title_fullStr In Peripheral Blood Mononuclear Cells Helicobacter pylori Induces the Secretion of Soluble and Exosomal Cytokines Related to Carcinogenesis
title_full_unstemmed In Peripheral Blood Mononuclear Cells Helicobacter pylori Induces the Secretion of Soluble and Exosomal Cytokines Related to Carcinogenesis
title_short In Peripheral Blood Mononuclear Cells Helicobacter pylori Induces the Secretion of Soluble and Exosomal Cytokines Related to Carcinogenesis
title_sort in peripheral blood mononuclear cells helicobacter pylori induces the secretion of soluble and exosomal cytokines related to carcinogenesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9368997/
https://www.ncbi.nlm.nih.gov/pubmed/35955936
http://dx.doi.org/10.3390/ijms23158801
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