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Novel Antibiofilm Inhibitor Ginkgetin as an Antibacterial Synergist against Escherichia coli
As an opportunistic pathogen, Escherichia coli (E. coli) forms biofilm that increases the virulence of bacteria and antibiotic resistance, posing a serious threat to human and animal health. Recently, ginkgetin (Gin) has been discovered to have antiinflammatory, antioxidant, and antitumor properties...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9369100/ https://www.ncbi.nlm.nih.gov/pubmed/35955943 http://dx.doi.org/10.3390/ijms23158809 |
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author | Bai, Yubin Wang, Weiwei Shi, Mengyan Wei, Xiaojuan Zhou, Xuzheng Li, Bing Zhang, Jiyu |
author_facet | Bai, Yubin Wang, Weiwei Shi, Mengyan Wei, Xiaojuan Zhou, Xuzheng Li, Bing Zhang, Jiyu |
author_sort | Bai, Yubin |
collection | PubMed |
description | As an opportunistic pathogen, Escherichia coli (E. coli) forms biofilm that increases the virulence of bacteria and antibiotic resistance, posing a serious threat to human and animal health. Recently, ginkgetin (Gin) has been discovered to have antiinflammatory, antioxidant, and antitumor properties. In the present study, we evaluated the antibiofilm and antibacterial synergist of Gin against E. coli. Additionally, Alamar Blue assay combined with confocal laser scanning microscope (CLSM) and crystal violet (CV) staining was used to evaluate the effect of antibiofilm and antibacterial synergist against E. coli. Results showed that Gin reduces biofilm formation, exopolysaccharide (EPS) production, and motility against E. coli without limiting its growth and metabolic activity. Furthermore, we identified the inhibitory effect of Gin on AI-2 signaling molecule production, which showed apparent anti-quorum sensing (QS) properties. The qRT-PCR also indicated that Gin reduced the transcription of curli-related genes (csgA, csgD), flagella-formation genes (flhC, flhD, fliC, fliM), and QS-related genes (luxS, lsrB, lsrK, lsrR). Moreover, Gin showed obvious antibacterial synergism to overcome antibiotic resistance in E. coli with marketed antibiotics, including gentamicin, colistin B, and colistin E. These results suggested the potent antibiofilm and novel antibacterial synergist effect of Gin for treating E. coli infections. |
format | Online Article Text |
id | pubmed-9369100 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-93691002022-08-12 Novel Antibiofilm Inhibitor Ginkgetin as an Antibacterial Synergist against Escherichia coli Bai, Yubin Wang, Weiwei Shi, Mengyan Wei, Xiaojuan Zhou, Xuzheng Li, Bing Zhang, Jiyu Int J Mol Sci Article As an opportunistic pathogen, Escherichia coli (E. coli) forms biofilm that increases the virulence of bacteria and antibiotic resistance, posing a serious threat to human and animal health. Recently, ginkgetin (Gin) has been discovered to have antiinflammatory, antioxidant, and antitumor properties. In the present study, we evaluated the antibiofilm and antibacterial synergist of Gin against E. coli. Additionally, Alamar Blue assay combined with confocal laser scanning microscope (CLSM) and crystal violet (CV) staining was used to evaluate the effect of antibiofilm and antibacterial synergist against E. coli. Results showed that Gin reduces biofilm formation, exopolysaccharide (EPS) production, and motility against E. coli without limiting its growth and metabolic activity. Furthermore, we identified the inhibitory effect of Gin on AI-2 signaling molecule production, which showed apparent anti-quorum sensing (QS) properties. The qRT-PCR also indicated that Gin reduced the transcription of curli-related genes (csgA, csgD), flagella-formation genes (flhC, flhD, fliC, fliM), and QS-related genes (luxS, lsrB, lsrK, lsrR). Moreover, Gin showed obvious antibacterial synergism to overcome antibiotic resistance in E. coli with marketed antibiotics, including gentamicin, colistin B, and colistin E. These results suggested the potent antibiofilm and novel antibacterial synergist effect of Gin for treating E. coli infections. MDPI 2022-08-08 /pmc/articles/PMC9369100/ /pubmed/35955943 http://dx.doi.org/10.3390/ijms23158809 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Bai, Yubin Wang, Weiwei Shi, Mengyan Wei, Xiaojuan Zhou, Xuzheng Li, Bing Zhang, Jiyu Novel Antibiofilm Inhibitor Ginkgetin as an Antibacterial Synergist against Escherichia coli |
title | Novel Antibiofilm Inhibitor Ginkgetin as an Antibacterial Synergist against Escherichia coli |
title_full | Novel Antibiofilm Inhibitor Ginkgetin as an Antibacterial Synergist against Escherichia coli |
title_fullStr | Novel Antibiofilm Inhibitor Ginkgetin as an Antibacterial Synergist against Escherichia coli |
title_full_unstemmed | Novel Antibiofilm Inhibitor Ginkgetin as an Antibacterial Synergist against Escherichia coli |
title_short | Novel Antibiofilm Inhibitor Ginkgetin as an Antibacterial Synergist against Escherichia coli |
title_sort | novel antibiofilm inhibitor ginkgetin as an antibacterial synergist against escherichia coli |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9369100/ https://www.ncbi.nlm.nih.gov/pubmed/35955943 http://dx.doi.org/10.3390/ijms23158809 |
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