Cargando…

An Extensive Study of Phenol Red Thread as a Novel Non-Invasive Tear Sampling Technique for Proteomics Studies: Comparison with Two Commonly Used Methods

Tear samples are considered in recent publications as easily, noninvasively collectible information sources for precision medicine. Their complex composition may aid the identification of biomarkers and the monitoring of the effectiveness of treatments for the eye and systemic diseases. Sample colle...

Descripción completa

Detalles Bibliográficos
Autores principales: Kecskeméti, Gábor, Tóth-Molnár, Edit, Janáky, Tamás, Szabó, Zoltán
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9369290/
https://www.ncbi.nlm.nih.gov/pubmed/35955782
http://dx.doi.org/10.3390/ijms23158647
_version_ 1784766407695663104
author Kecskeméti, Gábor
Tóth-Molnár, Edit
Janáky, Tamás
Szabó, Zoltán
author_facet Kecskeméti, Gábor
Tóth-Molnár, Edit
Janáky, Tamás
Szabó, Zoltán
author_sort Kecskeméti, Gábor
collection PubMed
description Tear samples are considered in recent publications as easily, noninvasively collectible information sources for precision medicine. Their complex composition may aid the identification of biomarkers and the monitoring of the effectiveness of treatments for the eye and systemic diseases. Sample collection and processing are key steps in any analytical method, especially if subtle personal differences need to be detected. In this work, we evaluate the usability of a novel sample collection technique for human tear samples using phenol red threads (cotton thread treated with the pH indicator phenol red), which are efficiently used to measure tear volume in clinical diagnosis. The low invasiveness and low discomfort to the patients have already been demonstrated, but their applicability for proteomic sample collection has not yet been compared to other methods. We have shown, using various statistical approaches, the qualitative and quantitative differences in proteomic samples collected with this novel and two traditional methods using either glass capillaries or Schirmer’s paper strips. In all parameters studied, the phenol red threads proved to be equally or even more suitable than traditional methods. Based on detectability using different sampling methods, we have classified proteins in tear samples.
format Online
Article
Text
id pubmed-9369290
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-93692902022-08-12 An Extensive Study of Phenol Red Thread as a Novel Non-Invasive Tear Sampling Technique for Proteomics Studies: Comparison with Two Commonly Used Methods Kecskeméti, Gábor Tóth-Molnár, Edit Janáky, Tamás Szabó, Zoltán Int J Mol Sci Article Tear samples are considered in recent publications as easily, noninvasively collectible information sources for precision medicine. Their complex composition may aid the identification of biomarkers and the monitoring of the effectiveness of treatments for the eye and systemic diseases. Sample collection and processing are key steps in any analytical method, especially if subtle personal differences need to be detected. In this work, we evaluate the usability of a novel sample collection technique for human tear samples using phenol red threads (cotton thread treated with the pH indicator phenol red), which are efficiently used to measure tear volume in clinical diagnosis. The low invasiveness and low discomfort to the patients have already been demonstrated, but their applicability for proteomic sample collection has not yet been compared to other methods. We have shown, using various statistical approaches, the qualitative and quantitative differences in proteomic samples collected with this novel and two traditional methods using either glass capillaries or Schirmer’s paper strips. In all parameters studied, the phenol red threads proved to be equally or even more suitable than traditional methods. Based on detectability using different sampling methods, we have classified proteins in tear samples. MDPI 2022-08-03 /pmc/articles/PMC9369290/ /pubmed/35955782 http://dx.doi.org/10.3390/ijms23158647 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kecskeméti, Gábor
Tóth-Molnár, Edit
Janáky, Tamás
Szabó, Zoltán
An Extensive Study of Phenol Red Thread as a Novel Non-Invasive Tear Sampling Technique for Proteomics Studies: Comparison with Two Commonly Used Methods
title An Extensive Study of Phenol Red Thread as a Novel Non-Invasive Tear Sampling Technique for Proteomics Studies: Comparison with Two Commonly Used Methods
title_full An Extensive Study of Phenol Red Thread as a Novel Non-Invasive Tear Sampling Technique for Proteomics Studies: Comparison with Two Commonly Used Methods
title_fullStr An Extensive Study of Phenol Red Thread as a Novel Non-Invasive Tear Sampling Technique for Proteomics Studies: Comparison with Two Commonly Used Methods
title_full_unstemmed An Extensive Study of Phenol Red Thread as a Novel Non-Invasive Tear Sampling Technique for Proteomics Studies: Comparison with Two Commonly Used Methods
title_short An Extensive Study of Phenol Red Thread as a Novel Non-Invasive Tear Sampling Technique for Proteomics Studies: Comparison with Two Commonly Used Methods
title_sort extensive study of phenol red thread as a novel non-invasive tear sampling technique for proteomics studies: comparison with two commonly used methods
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9369290/
https://www.ncbi.nlm.nih.gov/pubmed/35955782
http://dx.doi.org/10.3390/ijms23158647
work_keys_str_mv AT kecskemetigabor anextensivestudyofphenolredthreadasanovelnoninvasivetearsamplingtechniqueforproteomicsstudiescomparisonwithtwocommonlyusedmethods
AT tothmolnaredit anextensivestudyofphenolredthreadasanovelnoninvasivetearsamplingtechniqueforproteomicsstudiescomparisonwithtwocommonlyusedmethods
AT janakytamas anextensivestudyofphenolredthreadasanovelnoninvasivetearsamplingtechniqueforproteomicsstudiescomparisonwithtwocommonlyusedmethods
AT szabozoltan anextensivestudyofphenolredthreadasanovelnoninvasivetearsamplingtechniqueforproteomicsstudiescomparisonwithtwocommonlyusedmethods
AT kecskemetigabor extensivestudyofphenolredthreadasanovelnoninvasivetearsamplingtechniqueforproteomicsstudiescomparisonwithtwocommonlyusedmethods
AT tothmolnaredit extensivestudyofphenolredthreadasanovelnoninvasivetearsamplingtechniqueforproteomicsstudiescomparisonwithtwocommonlyusedmethods
AT janakytamas extensivestudyofphenolredthreadasanovelnoninvasivetearsamplingtechniqueforproteomicsstudiescomparisonwithtwocommonlyusedmethods
AT szabozoltan extensivestudyofphenolredthreadasanovelnoninvasivetearsamplingtechniqueforproteomicsstudiescomparisonwithtwocommonlyusedmethods