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T-Cell Receptor Repertoire Sequencing and Its Applications: Focus on Infectious Diseases and Cancer
The immune system is a dynamic feature of each individual and a footprint of our unique internal and external exposures. Indeed, the type and level of exposure to physical and biological agents shape the development and behavior of this complex and diffuse system. Many pathological conditions depend...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9369427/ https://www.ncbi.nlm.nih.gov/pubmed/35955721 http://dx.doi.org/10.3390/ijms23158590 |
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author | Mazzotti, Lucia Gaimari, Anna Bravaccini, Sara Maltoni, Roberta Cerchione, Claudio Juan, Manel Navarro, Europa Azucena-Gonzalez Pasetto, Anna Nascimento Silva, Daniela Ancarani, Valentina Sambri, Vittorio Calabrò, Luana Martinelli, Giovanni Mazza, Massimiliano |
author_facet | Mazzotti, Lucia Gaimari, Anna Bravaccini, Sara Maltoni, Roberta Cerchione, Claudio Juan, Manel Navarro, Europa Azucena-Gonzalez Pasetto, Anna Nascimento Silva, Daniela Ancarani, Valentina Sambri, Vittorio Calabrò, Luana Martinelli, Giovanni Mazza, Massimiliano |
author_sort | Mazzotti, Lucia |
collection | PubMed |
description | The immune system is a dynamic feature of each individual and a footprint of our unique internal and external exposures. Indeed, the type and level of exposure to physical and biological agents shape the development and behavior of this complex and diffuse system. Many pathological conditions depend on how our immune system responds or does not respond to a pathogen or a disease or on how the regulation of immunity is altered by the disease itself. T-cells are important players in adaptive immunity and, together with B-cells, define specificity and monitor the internal and external signals that our organism perceives through its specific receptors, TCRs and BCRs, respectively. Today, high-throughput sequencing (HTS) applied to the TCR repertoire has opened a window of opportunity to disclose T-cell repertoire development and behavior down to the clonal level. Although TCR repertoire sequencing is easily accessible today, it is important to deeply understand the available technologies for choosing the best fit for the specific experimental needs and questions. Here, we provide an updated overview of TCR repertoire sequencing strategies, providers and applications to infectious diseases and cancer to guide researchers’ choice through the multitude of available options. The possibility of extending the TCR repertoire to HLA characterization will be of pivotal importance in the near future to understand how specific HLA genes shape T-cell responses in different pathological contexts and will add a level of comprehension that was unthinkable just a few years ago. |
format | Online Article Text |
id | pubmed-9369427 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-93694272022-08-12 T-Cell Receptor Repertoire Sequencing and Its Applications: Focus on Infectious Diseases and Cancer Mazzotti, Lucia Gaimari, Anna Bravaccini, Sara Maltoni, Roberta Cerchione, Claudio Juan, Manel Navarro, Europa Azucena-Gonzalez Pasetto, Anna Nascimento Silva, Daniela Ancarani, Valentina Sambri, Vittorio Calabrò, Luana Martinelli, Giovanni Mazza, Massimiliano Int J Mol Sci Review The immune system is a dynamic feature of each individual and a footprint of our unique internal and external exposures. Indeed, the type and level of exposure to physical and biological agents shape the development and behavior of this complex and diffuse system. Many pathological conditions depend on how our immune system responds or does not respond to a pathogen or a disease or on how the regulation of immunity is altered by the disease itself. T-cells are important players in adaptive immunity and, together with B-cells, define specificity and monitor the internal and external signals that our organism perceives through its specific receptors, TCRs and BCRs, respectively. Today, high-throughput sequencing (HTS) applied to the TCR repertoire has opened a window of opportunity to disclose T-cell repertoire development and behavior down to the clonal level. Although TCR repertoire sequencing is easily accessible today, it is important to deeply understand the available technologies for choosing the best fit for the specific experimental needs and questions. Here, we provide an updated overview of TCR repertoire sequencing strategies, providers and applications to infectious diseases and cancer to guide researchers’ choice through the multitude of available options. The possibility of extending the TCR repertoire to HLA characterization will be of pivotal importance in the near future to understand how specific HLA genes shape T-cell responses in different pathological contexts and will add a level of comprehension that was unthinkable just a few years ago. MDPI 2022-08-02 /pmc/articles/PMC9369427/ /pubmed/35955721 http://dx.doi.org/10.3390/ijms23158590 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Mazzotti, Lucia Gaimari, Anna Bravaccini, Sara Maltoni, Roberta Cerchione, Claudio Juan, Manel Navarro, Europa Azucena-Gonzalez Pasetto, Anna Nascimento Silva, Daniela Ancarani, Valentina Sambri, Vittorio Calabrò, Luana Martinelli, Giovanni Mazza, Massimiliano T-Cell Receptor Repertoire Sequencing and Its Applications: Focus on Infectious Diseases and Cancer |
title | T-Cell Receptor Repertoire Sequencing and Its Applications: Focus on Infectious Diseases and Cancer |
title_full | T-Cell Receptor Repertoire Sequencing and Its Applications: Focus on Infectious Diseases and Cancer |
title_fullStr | T-Cell Receptor Repertoire Sequencing and Its Applications: Focus on Infectious Diseases and Cancer |
title_full_unstemmed | T-Cell Receptor Repertoire Sequencing and Its Applications: Focus on Infectious Diseases and Cancer |
title_short | T-Cell Receptor Repertoire Sequencing and Its Applications: Focus on Infectious Diseases and Cancer |
title_sort | t-cell receptor repertoire sequencing and its applications: focus on infectious diseases and cancer |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9369427/ https://www.ncbi.nlm.nih.gov/pubmed/35955721 http://dx.doi.org/10.3390/ijms23158590 |
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