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Germanium Nanoparticles Prepared by Laser Ablation in Low Pressure Helium and Nitrogen Atmosphere for Biophotonic Applications
Due to particular physico-chemical characteristics and prominent optical properties, nanostructured germanium (Ge) appears as a promising material for biomedical applications, but its use in biological systems has been limited so far due to the difficulty of preparation of Ge nanostructures in a pur...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9369467/ https://www.ncbi.nlm.nih.gov/pubmed/35955245 http://dx.doi.org/10.3390/ma15155308 |
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author | Fronya, Anastasiya A. Antonenko, Sergey V. Karpov, Nikita V. Pokryshkin, Nikolay S. Eremina, Anna S. Yakunin, Valery G. Kharin, Alexander Yu. Syuy, Alexander V. Volkov, Valentin S. Dombrovska, Yaroslava Garmash, Alexander A. Kargin, Nikolay I. Klimentov, Sergey M. Timoshenko, Victor Yu. Kabashin, Andrei V. |
author_facet | Fronya, Anastasiya A. Antonenko, Sergey V. Karpov, Nikita V. Pokryshkin, Nikolay S. Eremina, Anna S. Yakunin, Valery G. Kharin, Alexander Yu. Syuy, Alexander V. Volkov, Valentin S. Dombrovska, Yaroslava Garmash, Alexander A. Kargin, Nikolay I. Klimentov, Sergey M. Timoshenko, Victor Yu. Kabashin, Andrei V. |
author_sort | Fronya, Anastasiya A. |
collection | PubMed |
description | Due to particular physico-chemical characteristics and prominent optical properties, nanostructured germanium (Ge) appears as a promising material for biomedical applications, but its use in biological systems has been limited so far due to the difficulty of preparation of Ge nanostructures in a pure, uncontaminated state. Here, we explored the fabrication of Ge nanoparticles (NPs) using methods of pulsed laser ablation in ambient gas (He or He-N(2) mixtures) maintained at low residual pressures (1–5 Torr). We show that the ablated material can be deposited on a substrate (silicon wafer in our case) to form a nanostructured thin film, which can then be ground in ethanol by ultrasound to form a stable suspension of Ge NPs. It was found that these formed NPs have a wide size dispersion, with sizes between a few nm and hundreds of nm, while a subsequent centrifugation step renders possible the selection of one or another NP size fraction. Structural characterization of NPs showed that they are composed of aggregations of Ge crystals, covered by an oxide shell. Solutions of the prepared NPs exhibited largely dominating photoluminescence (PL) around 450 nm, attributed to defects in the germanium oxide shell, while a separated fraction of relatively small (5–10 nm) NPs exhibited a red-shifted PL band around 725 nm under 633 nm excitation, which could be attributed to quantum confinement effects. It was also found that the formed NPs exhibit high absorption in the visible and near-IR spectral ranges and can be strongly heated under photoexcitation in the region of relative tissue transparency, which opens access to phototherapy functionality. Combining imaging and therapy functionalities in the biological transparency window, laser-synthesized Ge NPs present a novel promising object for cancer theranostics. |
format | Online Article Text |
id | pubmed-9369467 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-93694672022-08-12 Germanium Nanoparticles Prepared by Laser Ablation in Low Pressure Helium and Nitrogen Atmosphere for Biophotonic Applications Fronya, Anastasiya A. Antonenko, Sergey V. Karpov, Nikita V. Pokryshkin, Nikolay S. Eremina, Anna S. Yakunin, Valery G. Kharin, Alexander Yu. Syuy, Alexander V. Volkov, Valentin S. Dombrovska, Yaroslava Garmash, Alexander A. Kargin, Nikolay I. Klimentov, Sergey M. Timoshenko, Victor Yu. Kabashin, Andrei V. Materials (Basel) Article Due to particular physico-chemical characteristics and prominent optical properties, nanostructured germanium (Ge) appears as a promising material for biomedical applications, but its use in biological systems has been limited so far due to the difficulty of preparation of Ge nanostructures in a pure, uncontaminated state. Here, we explored the fabrication of Ge nanoparticles (NPs) using methods of pulsed laser ablation in ambient gas (He or He-N(2) mixtures) maintained at low residual pressures (1–5 Torr). We show that the ablated material can be deposited on a substrate (silicon wafer in our case) to form a nanostructured thin film, which can then be ground in ethanol by ultrasound to form a stable suspension of Ge NPs. It was found that these formed NPs have a wide size dispersion, with sizes between a few nm and hundreds of nm, while a subsequent centrifugation step renders possible the selection of one or another NP size fraction. Structural characterization of NPs showed that they are composed of aggregations of Ge crystals, covered by an oxide shell. Solutions of the prepared NPs exhibited largely dominating photoluminescence (PL) around 450 nm, attributed to defects in the germanium oxide shell, while a separated fraction of relatively small (5–10 nm) NPs exhibited a red-shifted PL band around 725 nm under 633 nm excitation, which could be attributed to quantum confinement effects. It was also found that the formed NPs exhibit high absorption in the visible and near-IR spectral ranges and can be strongly heated under photoexcitation in the region of relative tissue transparency, which opens access to phototherapy functionality. Combining imaging and therapy functionalities in the biological transparency window, laser-synthesized Ge NPs present a novel promising object for cancer theranostics. MDPI 2022-08-02 /pmc/articles/PMC9369467/ /pubmed/35955245 http://dx.doi.org/10.3390/ma15155308 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Fronya, Anastasiya A. Antonenko, Sergey V. Karpov, Nikita V. Pokryshkin, Nikolay S. Eremina, Anna S. Yakunin, Valery G. Kharin, Alexander Yu. Syuy, Alexander V. Volkov, Valentin S. Dombrovska, Yaroslava Garmash, Alexander A. Kargin, Nikolay I. Klimentov, Sergey M. Timoshenko, Victor Yu. Kabashin, Andrei V. Germanium Nanoparticles Prepared by Laser Ablation in Low Pressure Helium and Nitrogen Atmosphere for Biophotonic Applications |
title | Germanium Nanoparticles Prepared by Laser Ablation in Low Pressure Helium and Nitrogen Atmosphere for Biophotonic Applications |
title_full | Germanium Nanoparticles Prepared by Laser Ablation in Low Pressure Helium and Nitrogen Atmosphere for Biophotonic Applications |
title_fullStr | Germanium Nanoparticles Prepared by Laser Ablation in Low Pressure Helium and Nitrogen Atmosphere for Biophotonic Applications |
title_full_unstemmed | Germanium Nanoparticles Prepared by Laser Ablation in Low Pressure Helium and Nitrogen Atmosphere for Biophotonic Applications |
title_short | Germanium Nanoparticles Prepared by Laser Ablation in Low Pressure Helium and Nitrogen Atmosphere for Biophotonic Applications |
title_sort | germanium nanoparticles prepared by laser ablation in low pressure helium and nitrogen atmosphere for biophotonic applications |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9369467/ https://www.ncbi.nlm.nih.gov/pubmed/35955245 http://dx.doi.org/10.3390/ma15155308 |
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