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I-FABP as a Potential Marker for Intestinal Barrier Loss in Porcine Polytrauma
Polytrauma and concomitant hemorrhagic shock can lead to intestinal damage and subsequent multiple organ dysfunction syndrome. The intestinal fatty acid-binding protein (I-FABP) is expressed in the intestine and appears quickly in the circulation after intestinal epithelial cell damage. This porcine...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9369469/ https://www.ncbi.nlm.nih.gov/pubmed/35956214 http://dx.doi.org/10.3390/jcm11154599 |
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author | Vollrath, Jan Tilmann Klingebiel, Felix Bläsius, Felix Greven, Johannes Bolierakis, Eftychios Nowak, Aleksander J. Simic, Marija Hildebrand, Frank Marzi, Ingo Relja, Borna |
author_facet | Vollrath, Jan Tilmann Klingebiel, Felix Bläsius, Felix Greven, Johannes Bolierakis, Eftychios Nowak, Aleksander J. Simic, Marija Hildebrand, Frank Marzi, Ingo Relja, Borna |
author_sort | Vollrath, Jan Tilmann |
collection | PubMed |
description | Polytrauma and concomitant hemorrhagic shock can lead to intestinal damage and subsequent multiple organ dysfunction syndrome. The intestinal fatty acid-binding protein (I-FABP) is expressed in the intestine and appears quickly in the circulation after intestinal epithelial cell damage. This porcine animal study investigates the I-FABP dynamics in plasma and urine after polytrauma. Furthermore, it evaluates to what extent I-FABP can also act as a marker of intestinal damage in a porcine polytrauma model. Eight pigs (Sus scrofa) were subjected to polytrauma which consisted of lung contusion, tibial fracture, liver laceration, and hemorrhagic shock followed by blood and fluid resuscitation and fracture fixation with an external fixator. Eight sham animals were identically instrumented but not injured. Afterwards, intensive care treatment including mechanical ventilation for 72 h followed. I-FABP levels in blood and urine were determined by ELISA. In addition, immunohistological staining for I-FABP, active caspase-3 and myeloperoxidase were performed after 72 h. Plasma and urine I-FABP levels were significantly increased shortly after trauma. I-FABP expression in intestinal tissue showed significantly lower expression in polytraumatized animals vs. sham. Caspase-3 and myeloperoxidase expression in the immunohistological examination were significantly higher in the jejunum and ileum of polytraumatized animals compared to sham animals. This study confirms a loss of intestinal barrier after polytrauma which is indicated by increased I-FABP levels in plasma and urine as well as decreased I-FABP levels in immunohistological staining of the intestine. |
format | Online Article Text |
id | pubmed-9369469 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-93694692022-08-12 I-FABP as a Potential Marker for Intestinal Barrier Loss in Porcine Polytrauma Vollrath, Jan Tilmann Klingebiel, Felix Bläsius, Felix Greven, Johannes Bolierakis, Eftychios Nowak, Aleksander J. Simic, Marija Hildebrand, Frank Marzi, Ingo Relja, Borna J Clin Med Article Polytrauma and concomitant hemorrhagic shock can lead to intestinal damage and subsequent multiple organ dysfunction syndrome. The intestinal fatty acid-binding protein (I-FABP) is expressed in the intestine and appears quickly in the circulation after intestinal epithelial cell damage. This porcine animal study investigates the I-FABP dynamics in plasma and urine after polytrauma. Furthermore, it evaluates to what extent I-FABP can also act as a marker of intestinal damage in a porcine polytrauma model. Eight pigs (Sus scrofa) were subjected to polytrauma which consisted of lung contusion, tibial fracture, liver laceration, and hemorrhagic shock followed by blood and fluid resuscitation and fracture fixation with an external fixator. Eight sham animals were identically instrumented but not injured. Afterwards, intensive care treatment including mechanical ventilation for 72 h followed. I-FABP levels in blood and urine were determined by ELISA. In addition, immunohistological staining for I-FABP, active caspase-3 and myeloperoxidase were performed after 72 h. Plasma and urine I-FABP levels were significantly increased shortly after trauma. I-FABP expression in intestinal tissue showed significantly lower expression in polytraumatized animals vs. sham. Caspase-3 and myeloperoxidase expression in the immunohistological examination were significantly higher in the jejunum and ileum of polytraumatized animals compared to sham animals. This study confirms a loss of intestinal barrier after polytrauma which is indicated by increased I-FABP levels in plasma and urine as well as decreased I-FABP levels in immunohistological staining of the intestine. MDPI 2022-08-07 /pmc/articles/PMC9369469/ /pubmed/35956214 http://dx.doi.org/10.3390/jcm11154599 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Vollrath, Jan Tilmann Klingebiel, Felix Bläsius, Felix Greven, Johannes Bolierakis, Eftychios Nowak, Aleksander J. Simic, Marija Hildebrand, Frank Marzi, Ingo Relja, Borna I-FABP as a Potential Marker for Intestinal Barrier Loss in Porcine Polytrauma |
title | I-FABP as a Potential Marker for Intestinal Barrier Loss in Porcine Polytrauma |
title_full | I-FABP as a Potential Marker for Intestinal Barrier Loss in Porcine Polytrauma |
title_fullStr | I-FABP as a Potential Marker for Intestinal Barrier Loss in Porcine Polytrauma |
title_full_unstemmed | I-FABP as a Potential Marker for Intestinal Barrier Loss in Porcine Polytrauma |
title_short | I-FABP as a Potential Marker for Intestinal Barrier Loss in Porcine Polytrauma |
title_sort | i-fabp as a potential marker for intestinal barrier loss in porcine polytrauma |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9369469/ https://www.ncbi.nlm.nih.gov/pubmed/35956214 http://dx.doi.org/10.3390/jcm11154599 |
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