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I-FABP as a Potential Marker for Intestinal Barrier Loss in Porcine Polytrauma

Polytrauma and concomitant hemorrhagic shock can lead to intestinal damage and subsequent multiple organ dysfunction syndrome. The intestinal fatty acid-binding protein (I-FABP) is expressed in the intestine and appears quickly in the circulation after intestinal epithelial cell damage. This porcine...

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Autores principales: Vollrath, Jan Tilmann, Klingebiel, Felix, Bläsius, Felix, Greven, Johannes, Bolierakis, Eftychios, Nowak, Aleksander J., Simic, Marija, Hildebrand, Frank, Marzi, Ingo, Relja, Borna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9369469/
https://www.ncbi.nlm.nih.gov/pubmed/35956214
http://dx.doi.org/10.3390/jcm11154599
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author Vollrath, Jan Tilmann
Klingebiel, Felix
Bläsius, Felix
Greven, Johannes
Bolierakis, Eftychios
Nowak, Aleksander J.
Simic, Marija
Hildebrand, Frank
Marzi, Ingo
Relja, Borna
author_facet Vollrath, Jan Tilmann
Klingebiel, Felix
Bläsius, Felix
Greven, Johannes
Bolierakis, Eftychios
Nowak, Aleksander J.
Simic, Marija
Hildebrand, Frank
Marzi, Ingo
Relja, Borna
author_sort Vollrath, Jan Tilmann
collection PubMed
description Polytrauma and concomitant hemorrhagic shock can lead to intestinal damage and subsequent multiple organ dysfunction syndrome. The intestinal fatty acid-binding protein (I-FABP) is expressed in the intestine and appears quickly in the circulation after intestinal epithelial cell damage. This porcine animal study investigates the I-FABP dynamics in plasma and urine after polytrauma. Furthermore, it evaluates to what extent I-FABP can also act as a marker of intestinal damage in a porcine polytrauma model. Eight pigs (Sus scrofa) were subjected to polytrauma which consisted of lung contusion, tibial fracture, liver laceration, and hemorrhagic shock followed by blood and fluid resuscitation and fracture fixation with an external fixator. Eight sham animals were identically instrumented but not injured. Afterwards, intensive care treatment including mechanical ventilation for 72 h followed. I-FABP levels in blood and urine were determined by ELISA. In addition, immunohistological staining for I-FABP, active caspase-3 and myeloperoxidase were performed after 72 h. Plasma and urine I-FABP levels were significantly increased shortly after trauma. I-FABP expression in intestinal tissue showed significantly lower expression in polytraumatized animals vs. sham. Caspase-3 and myeloperoxidase expression in the immunohistological examination were significantly higher in the jejunum and ileum of polytraumatized animals compared to sham animals. This study confirms a loss of intestinal barrier after polytrauma which is indicated by increased I-FABP levels in plasma and urine as well as decreased I-FABP levels in immunohistological staining of the intestine.
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spelling pubmed-93694692022-08-12 I-FABP as a Potential Marker for Intestinal Barrier Loss in Porcine Polytrauma Vollrath, Jan Tilmann Klingebiel, Felix Bläsius, Felix Greven, Johannes Bolierakis, Eftychios Nowak, Aleksander J. Simic, Marija Hildebrand, Frank Marzi, Ingo Relja, Borna J Clin Med Article Polytrauma and concomitant hemorrhagic shock can lead to intestinal damage and subsequent multiple organ dysfunction syndrome. The intestinal fatty acid-binding protein (I-FABP) is expressed in the intestine and appears quickly in the circulation after intestinal epithelial cell damage. This porcine animal study investigates the I-FABP dynamics in plasma and urine after polytrauma. Furthermore, it evaluates to what extent I-FABP can also act as a marker of intestinal damage in a porcine polytrauma model. Eight pigs (Sus scrofa) were subjected to polytrauma which consisted of lung contusion, tibial fracture, liver laceration, and hemorrhagic shock followed by blood and fluid resuscitation and fracture fixation with an external fixator. Eight sham animals were identically instrumented but not injured. Afterwards, intensive care treatment including mechanical ventilation for 72 h followed. I-FABP levels in blood and urine were determined by ELISA. In addition, immunohistological staining for I-FABP, active caspase-3 and myeloperoxidase were performed after 72 h. Plasma and urine I-FABP levels were significantly increased shortly after trauma. I-FABP expression in intestinal tissue showed significantly lower expression in polytraumatized animals vs. sham. Caspase-3 and myeloperoxidase expression in the immunohistological examination were significantly higher in the jejunum and ileum of polytraumatized animals compared to sham animals. This study confirms a loss of intestinal barrier after polytrauma which is indicated by increased I-FABP levels in plasma and urine as well as decreased I-FABP levels in immunohistological staining of the intestine. MDPI 2022-08-07 /pmc/articles/PMC9369469/ /pubmed/35956214 http://dx.doi.org/10.3390/jcm11154599 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Vollrath, Jan Tilmann
Klingebiel, Felix
Bläsius, Felix
Greven, Johannes
Bolierakis, Eftychios
Nowak, Aleksander J.
Simic, Marija
Hildebrand, Frank
Marzi, Ingo
Relja, Borna
I-FABP as a Potential Marker for Intestinal Barrier Loss in Porcine Polytrauma
title I-FABP as a Potential Marker for Intestinal Barrier Loss in Porcine Polytrauma
title_full I-FABP as a Potential Marker for Intestinal Barrier Loss in Porcine Polytrauma
title_fullStr I-FABP as a Potential Marker for Intestinal Barrier Loss in Porcine Polytrauma
title_full_unstemmed I-FABP as a Potential Marker for Intestinal Barrier Loss in Porcine Polytrauma
title_short I-FABP as a Potential Marker for Intestinal Barrier Loss in Porcine Polytrauma
title_sort i-fabp as a potential marker for intestinal barrier loss in porcine polytrauma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9369469/
https://www.ncbi.nlm.nih.gov/pubmed/35956214
http://dx.doi.org/10.3390/jcm11154599
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