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Quality Evaluation of Tetrastigmae Radix from Two Different Habitats Based on Simultaneous Determination of Multiple Bioactive Constituents Combined with Multivariate Statistical Analysis

Tetrastigmae Radix, also known as Sanyeqing (SYQ) in Chinese, is an important traditional Chinese medicine with a long history. Tetrastigma hemsleyanum Diels et Gilg mainly grows in the south of the Yangtze River and is widely distributed. The content of bioactive constituents in SYQ varies greatly...

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Autores principales: Chen, Haijie, Zhou, Yongyi, Xue, Jia, Yuan, Jiahuan, Cai, Zhichen, Wu, Nan, Zou, Lisi, Yin, Shengxin, Yang, Wei, Liu, Xunhong, Cheng, Jianming, Tang, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9369617/
https://www.ncbi.nlm.nih.gov/pubmed/35956760
http://dx.doi.org/10.3390/molecules27154813
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author Chen, Haijie
Zhou, Yongyi
Xue, Jia
Yuan, Jiahuan
Cai, Zhichen
Wu, Nan
Zou, Lisi
Yin, Shengxin
Yang, Wei
Liu, Xunhong
Cheng, Jianming
Tang, Li
author_facet Chen, Haijie
Zhou, Yongyi
Xue, Jia
Yuan, Jiahuan
Cai, Zhichen
Wu, Nan
Zou, Lisi
Yin, Shengxin
Yang, Wei
Liu, Xunhong
Cheng, Jianming
Tang, Li
author_sort Chen, Haijie
collection PubMed
description Tetrastigmae Radix, also known as Sanyeqing (SYQ) in Chinese, is an important traditional Chinese medicine with a long history. Tetrastigma hemsleyanum Diels et Gilg mainly grows in the south of the Yangtze River and is widely distributed. The content of bioactive constituents in SYQ varies greatly in different habitats, and there are obvious differences in the content of bioactive constituents between southwestern SYQ (WS) and southeastern SYQ (ES). To distinguish and evaluate the quality of ES and WS, an analytical method based on ultrafast performance liquid chromatography coupled with triple quadrupole-linear ion trap mass spectrometry (UFLC-QTRAP-MS/MS) was established for the simultaneous determination of 60 constituents including 25 flavonoids, 9 phenolic acids, 15 amino acids, and 11 nucleosides in 47 samples from ES and WS. In addition, orthogonal partial least squares discriminant analysis (OPLS-DA), t-test, and gray correlation analysis (GRA) were used to discriminate and evaluate the ES and WS samples based on the contents of 60 constituents. The results showed that there were significant differences in the bioactive constituents between ES and WS, and ES was superior to WS in terms of quality evaluation. This study not only provides basic information for differentiating ES and WS but also provides a new perspective for the comprehensive evaluation and quality control of SYQ from two different habitats.
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spelling pubmed-93696172022-08-12 Quality Evaluation of Tetrastigmae Radix from Two Different Habitats Based on Simultaneous Determination of Multiple Bioactive Constituents Combined with Multivariate Statistical Analysis Chen, Haijie Zhou, Yongyi Xue, Jia Yuan, Jiahuan Cai, Zhichen Wu, Nan Zou, Lisi Yin, Shengxin Yang, Wei Liu, Xunhong Cheng, Jianming Tang, Li Molecules Article Tetrastigmae Radix, also known as Sanyeqing (SYQ) in Chinese, is an important traditional Chinese medicine with a long history. Tetrastigma hemsleyanum Diels et Gilg mainly grows in the south of the Yangtze River and is widely distributed. The content of bioactive constituents in SYQ varies greatly in different habitats, and there are obvious differences in the content of bioactive constituents between southwestern SYQ (WS) and southeastern SYQ (ES). To distinguish and evaluate the quality of ES and WS, an analytical method based on ultrafast performance liquid chromatography coupled with triple quadrupole-linear ion trap mass spectrometry (UFLC-QTRAP-MS/MS) was established for the simultaneous determination of 60 constituents including 25 flavonoids, 9 phenolic acids, 15 amino acids, and 11 nucleosides in 47 samples from ES and WS. In addition, orthogonal partial least squares discriminant analysis (OPLS-DA), t-test, and gray correlation analysis (GRA) were used to discriminate and evaluate the ES and WS samples based on the contents of 60 constituents. The results showed that there were significant differences in the bioactive constituents between ES and WS, and ES was superior to WS in terms of quality evaluation. This study not only provides basic information for differentiating ES and WS but also provides a new perspective for the comprehensive evaluation and quality control of SYQ from two different habitats. MDPI 2022-07-27 /pmc/articles/PMC9369617/ /pubmed/35956760 http://dx.doi.org/10.3390/molecules27154813 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chen, Haijie
Zhou, Yongyi
Xue, Jia
Yuan, Jiahuan
Cai, Zhichen
Wu, Nan
Zou, Lisi
Yin, Shengxin
Yang, Wei
Liu, Xunhong
Cheng, Jianming
Tang, Li
Quality Evaluation of Tetrastigmae Radix from Two Different Habitats Based on Simultaneous Determination of Multiple Bioactive Constituents Combined with Multivariate Statistical Analysis
title Quality Evaluation of Tetrastigmae Radix from Two Different Habitats Based on Simultaneous Determination of Multiple Bioactive Constituents Combined with Multivariate Statistical Analysis
title_full Quality Evaluation of Tetrastigmae Radix from Two Different Habitats Based on Simultaneous Determination of Multiple Bioactive Constituents Combined with Multivariate Statistical Analysis
title_fullStr Quality Evaluation of Tetrastigmae Radix from Two Different Habitats Based on Simultaneous Determination of Multiple Bioactive Constituents Combined with Multivariate Statistical Analysis
title_full_unstemmed Quality Evaluation of Tetrastigmae Radix from Two Different Habitats Based on Simultaneous Determination of Multiple Bioactive Constituents Combined with Multivariate Statistical Analysis
title_short Quality Evaluation of Tetrastigmae Radix from Two Different Habitats Based on Simultaneous Determination of Multiple Bioactive Constituents Combined with Multivariate Statistical Analysis
title_sort quality evaluation of tetrastigmae radix from two different habitats based on simultaneous determination of multiple bioactive constituents combined with multivariate statistical analysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9369617/
https://www.ncbi.nlm.nih.gov/pubmed/35956760
http://dx.doi.org/10.3390/molecules27154813
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