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Identification of Novel Broad-Spectrum Leaf Rust Resistance Sources from Khapli Wheat Landraces

Wheat leaf rust caused by Puccinia triticina Eriks is an important disease that causes yield losses of up to 40% in susceptible varieties. Tetraploid emmer wheat (T. turgidum ssp. Dicoccum), commonly called Khapli wheat in India, is known to have evolved from wild emmer (Triticum turgidum var. dicoc...

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Autores principales: Raghunandan, K., Tanwar, Jatin, Patil, Shivanagouda N., Chandra, Ajay Kumar, Tyagi, Sandhya, Agarwal, Priyanka, Mallick, Niharika, Murukan, Niranjana, Kumari, Jyoti, Sahu, Tanmaya Kumar, Jacob, Sherry R., Kumar, Atul, Yadav, Suresh, Nyamgoud, Sneha, Vinod, Singh, Amit Kumar, Jha, Shailendra Kumar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9370231/
https://www.ncbi.nlm.nih.gov/pubmed/35956445
http://dx.doi.org/10.3390/plants11151965
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author Raghunandan, K.
Tanwar, Jatin
Patil, Shivanagouda N.
Chandra, Ajay Kumar
Tyagi, Sandhya
Agarwal, Priyanka
Mallick, Niharika
Murukan, Niranjana
Kumari, Jyoti
Sahu, Tanmaya Kumar
Jacob, Sherry R.
Kumar, Atul
Yadav, Suresh
Nyamgoud, Sneha
Vinod,
Singh, Amit Kumar
Jha, Shailendra Kumar
author_facet Raghunandan, K.
Tanwar, Jatin
Patil, Shivanagouda N.
Chandra, Ajay Kumar
Tyagi, Sandhya
Agarwal, Priyanka
Mallick, Niharika
Murukan, Niranjana
Kumari, Jyoti
Sahu, Tanmaya Kumar
Jacob, Sherry R.
Kumar, Atul
Yadav, Suresh
Nyamgoud, Sneha
Vinod,
Singh, Amit Kumar
Jha, Shailendra Kumar
author_sort Raghunandan, K.
collection PubMed
description Wheat leaf rust caused by Puccinia triticina Eriks is an important disease that causes yield losses of up to 40% in susceptible varieties. Tetraploid emmer wheat (T. turgidum ssp. Dicoccum), commonly called Khapli wheat in India, is known to have evolved from wild emmer (Triticum turgidum var. dicoccoides), and harbors a good number of leaf rust resistance genes. In the present study, we are reporting on the screening of one hundred and twenty-three dicoccum wheat germplasm accessions against the leaf rust pathotype 77-5. Among these, an average of 45.50% of the germplasms were resistant, 46.74% were susceptible, and 8.53% had mesothetic reactions. Further, selected germplasm lines with accession numbers IC138898, IC47022, IC535116, IC535133, IC535139, IC551396, and IC534144 showed high level of resistance against the eighteen prevalent pathotypes. The infection type varied from “;”, “;N”, “;N1” to “;NC”. PCR-based analysis of the resistant dicoccum lines with SSR marker gwm508 linked to the Lr53 gene, a leaf rust resistance gene effective against all the prevalent pathotypes of leaf rust in India and identified from a T. turgidum var. dicoccoides germplasm, indicated that Lr53 is not present in the selected accessions. Moreover, we have also generated 35K SNP genotyping data of seven lines and the susceptible control, Mandsaur Local, to study their relationships. The GDIRT tool based on homozygous genotypic differences revealed that the seven genotypes are unique to each other and may carry different resistance genes for leaf rust.
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spelling pubmed-93702312022-08-12 Identification of Novel Broad-Spectrum Leaf Rust Resistance Sources from Khapli Wheat Landraces Raghunandan, K. Tanwar, Jatin Patil, Shivanagouda N. Chandra, Ajay Kumar Tyagi, Sandhya Agarwal, Priyanka Mallick, Niharika Murukan, Niranjana Kumari, Jyoti Sahu, Tanmaya Kumar Jacob, Sherry R. Kumar, Atul Yadav, Suresh Nyamgoud, Sneha Vinod, Singh, Amit Kumar Jha, Shailendra Kumar Plants (Basel) Article Wheat leaf rust caused by Puccinia triticina Eriks is an important disease that causes yield losses of up to 40% in susceptible varieties. Tetraploid emmer wheat (T. turgidum ssp. Dicoccum), commonly called Khapli wheat in India, is known to have evolved from wild emmer (Triticum turgidum var. dicoccoides), and harbors a good number of leaf rust resistance genes. In the present study, we are reporting on the screening of one hundred and twenty-three dicoccum wheat germplasm accessions against the leaf rust pathotype 77-5. Among these, an average of 45.50% of the germplasms were resistant, 46.74% were susceptible, and 8.53% had mesothetic reactions. Further, selected germplasm lines with accession numbers IC138898, IC47022, IC535116, IC535133, IC535139, IC551396, and IC534144 showed high level of resistance against the eighteen prevalent pathotypes. The infection type varied from “;”, “;N”, “;N1” to “;NC”. PCR-based analysis of the resistant dicoccum lines with SSR marker gwm508 linked to the Lr53 gene, a leaf rust resistance gene effective against all the prevalent pathotypes of leaf rust in India and identified from a T. turgidum var. dicoccoides germplasm, indicated that Lr53 is not present in the selected accessions. Moreover, we have also generated 35K SNP genotyping data of seven lines and the susceptible control, Mandsaur Local, to study their relationships. The GDIRT tool based on homozygous genotypic differences revealed that the seven genotypes are unique to each other and may carry different resistance genes for leaf rust. MDPI 2022-07-28 /pmc/articles/PMC9370231/ /pubmed/35956445 http://dx.doi.org/10.3390/plants11151965 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Raghunandan, K.
Tanwar, Jatin
Patil, Shivanagouda N.
Chandra, Ajay Kumar
Tyagi, Sandhya
Agarwal, Priyanka
Mallick, Niharika
Murukan, Niranjana
Kumari, Jyoti
Sahu, Tanmaya Kumar
Jacob, Sherry R.
Kumar, Atul
Yadav, Suresh
Nyamgoud, Sneha
Vinod,
Singh, Amit Kumar
Jha, Shailendra Kumar
Identification of Novel Broad-Spectrum Leaf Rust Resistance Sources from Khapli Wheat Landraces
title Identification of Novel Broad-Spectrum Leaf Rust Resistance Sources from Khapli Wheat Landraces
title_full Identification of Novel Broad-Spectrum Leaf Rust Resistance Sources from Khapli Wheat Landraces
title_fullStr Identification of Novel Broad-Spectrum Leaf Rust Resistance Sources from Khapli Wheat Landraces
title_full_unstemmed Identification of Novel Broad-Spectrum Leaf Rust Resistance Sources from Khapli Wheat Landraces
title_short Identification of Novel Broad-Spectrum Leaf Rust Resistance Sources from Khapli Wheat Landraces
title_sort identification of novel broad-spectrum leaf rust resistance sources from khapli wheat landraces
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9370231/
https://www.ncbi.nlm.nih.gov/pubmed/35956445
http://dx.doi.org/10.3390/plants11151965
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