Cargando…

Metagenomics Approach to the Intestinal Microbiome Structure and Abundance in High-Fat-Diet-Induced Hyperlipidemic Rat Fed with (−)-Epigallocatechin-3-Gallate Nanoparticles

The effects of nanoparticles (NPs) on microbiota homeostasis and their physiological relevance are still unclear. Herein, we compared the modulation and consequent pharmacological effects of oral administration of (−)-epigallocatechin-3-gallate (EGCG)-loaded β-cyclodextrin (β-CD) NPs (EGCG@β-CD NPs)...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Zhiyin, Liu, Baogui, Gong, Zhihua, Huang, Hua, Gong, Yihui, Xiao, Wenjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9370321/
https://www.ncbi.nlm.nih.gov/pubmed/35956844
http://dx.doi.org/10.3390/molecules27154894
_version_ 1784766757974573056
author Chen, Zhiyin
Liu, Baogui
Gong, Zhihua
Huang, Hua
Gong, Yihui
Xiao, Wenjun
author_facet Chen, Zhiyin
Liu, Baogui
Gong, Zhihua
Huang, Hua
Gong, Yihui
Xiao, Wenjun
author_sort Chen, Zhiyin
collection PubMed
description The effects of nanoparticles (NPs) on microbiota homeostasis and their physiological relevance are still unclear. Herein, we compared the modulation and consequent pharmacological effects of oral administration of (−)-epigallocatechin-3-gallate (EGCG)-loaded β-cyclodextrin (β-CD) NPs (EGCG@β-CD NPs) and EGCG on gut microbiota. EGCG@β-CD NPs were prepared using self-assembly and their influence on the intestinal microbiome structure was analyzed using a metagenomics approach. The “Encapsulation efficiency (EE), particle size, polydispersity index (PDI), zeta potential” of EGCG@β-CD NPs were recorded as 98.27 ± 0.36%, 124.6 nm, 0.313 and –24.3 mV, respectively. Surface morphology of EGCG@β-CD NPs was observed as spherical. Fourier-transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and molecular docking studies confirmed that EGCG could be well encapsulated in β-CD and formed as EGCG@β-CD NPs. After being continuously administered EGCG@β-CD NPs for 8 weeks, the serum cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) and liver malondialdehyde (MDA) levels in the rats were significantly decreased, while the levels of catalase (CAT) and apolipoprotein-A1 (apo-A1) in the liver increased significantly in the hyperlipidemia model of rats, when compared to the high-fat-diet group. Furthermore, metagenomic analysis revealed that the ratio of Verrucomicrobia/Bacteroidetes was altered and Bacteroidetes decreased in the high-fat diet +200 mg/kg·bw EGCG@β-CD NPs group, while the abundance of Verrucomicrobia was significantly increased, especially Akkermansia muciniphila in rat feces. EGCG@β-CD NPs could be a promising EGCG delivery strategy to modulate the gut microbiota, enhancing its employment in the prevention of hyperlipidemia.
format Online
Article
Text
id pubmed-9370321
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-93703212022-08-12 Metagenomics Approach to the Intestinal Microbiome Structure and Abundance in High-Fat-Diet-Induced Hyperlipidemic Rat Fed with (−)-Epigallocatechin-3-Gallate Nanoparticles Chen, Zhiyin Liu, Baogui Gong, Zhihua Huang, Hua Gong, Yihui Xiao, Wenjun Molecules Article The effects of nanoparticles (NPs) on microbiota homeostasis and their physiological relevance are still unclear. Herein, we compared the modulation and consequent pharmacological effects of oral administration of (−)-epigallocatechin-3-gallate (EGCG)-loaded β-cyclodextrin (β-CD) NPs (EGCG@β-CD NPs) and EGCG on gut microbiota. EGCG@β-CD NPs were prepared using self-assembly and their influence on the intestinal microbiome structure was analyzed using a metagenomics approach. The “Encapsulation efficiency (EE), particle size, polydispersity index (PDI), zeta potential” of EGCG@β-CD NPs were recorded as 98.27 ± 0.36%, 124.6 nm, 0.313 and –24.3 mV, respectively. Surface morphology of EGCG@β-CD NPs was observed as spherical. Fourier-transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and molecular docking studies confirmed that EGCG could be well encapsulated in β-CD and formed as EGCG@β-CD NPs. After being continuously administered EGCG@β-CD NPs for 8 weeks, the serum cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) and liver malondialdehyde (MDA) levels in the rats were significantly decreased, while the levels of catalase (CAT) and apolipoprotein-A1 (apo-A1) in the liver increased significantly in the hyperlipidemia model of rats, when compared to the high-fat-diet group. Furthermore, metagenomic analysis revealed that the ratio of Verrucomicrobia/Bacteroidetes was altered and Bacteroidetes decreased in the high-fat diet +200 mg/kg·bw EGCG@β-CD NPs group, while the abundance of Verrucomicrobia was significantly increased, especially Akkermansia muciniphila in rat feces. EGCG@β-CD NPs could be a promising EGCG delivery strategy to modulate the gut microbiota, enhancing its employment in the prevention of hyperlipidemia. MDPI 2022-07-31 /pmc/articles/PMC9370321/ /pubmed/35956844 http://dx.doi.org/10.3390/molecules27154894 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chen, Zhiyin
Liu, Baogui
Gong, Zhihua
Huang, Hua
Gong, Yihui
Xiao, Wenjun
Metagenomics Approach to the Intestinal Microbiome Structure and Abundance in High-Fat-Diet-Induced Hyperlipidemic Rat Fed with (−)-Epigallocatechin-3-Gallate Nanoparticles
title Metagenomics Approach to the Intestinal Microbiome Structure and Abundance in High-Fat-Diet-Induced Hyperlipidemic Rat Fed with (−)-Epigallocatechin-3-Gallate Nanoparticles
title_full Metagenomics Approach to the Intestinal Microbiome Structure and Abundance in High-Fat-Diet-Induced Hyperlipidemic Rat Fed with (−)-Epigallocatechin-3-Gallate Nanoparticles
title_fullStr Metagenomics Approach to the Intestinal Microbiome Structure and Abundance in High-Fat-Diet-Induced Hyperlipidemic Rat Fed with (−)-Epigallocatechin-3-Gallate Nanoparticles
title_full_unstemmed Metagenomics Approach to the Intestinal Microbiome Structure and Abundance in High-Fat-Diet-Induced Hyperlipidemic Rat Fed with (−)-Epigallocatechin-3-Gallate Nanoparticles
title_short Metagenomics Approach to the Intestinal Microbiome Structure and Abundance in High-Fat-Diet-Induced Hyperlipidemic Rat Fed with (−)-Epigallocatechin-3-Gallate Nanoparticles
title_sort metagenomics approach to the intestinal microbiome structure and abundance in high-fat-diet-induced hyperlipidemic rat fed with (−)-epigallocatechin-3-gallate nanoparticles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9370321/
https://www.ncbi.nlm.nih.gov/pubmed/35956844
http://dx.doi.org/10.3390/molecules27154894
work_keys_str_mv AT chenzhiyin metagenomicsapproachtotheintestinalmicrobiomestructureandabundanceinhighfatdietinducedhyperlipidemicratfedwithepigallocatechin3gallatenanoparticles
AT liubaogui metagenomicsapproachtotheintestinalmicrobiomestructureandabundanceinhighfatdietinducedhyperlipidemicratfedwithepigallocatechin3gallatenanoparticles
AT gongzhihua metagenomicsapproachtotheintestinalmicrobiomestructureandabundanceinhighfatdietinducedhyperlipidemicratfedwithepigallocatechin3gallatenanoparticles
AT huanghua metagenomicsapproachtotheintestinalmicrobiomestructureandabundanceinhighfatdietinducedhyperlipidemicratfedwithepigallocatechin3gallatenanoparticles
AT gongyihui metagenomicsapproachtotheintestinalmicrobiomestructureandabundanceinhighfatdietinducedhyperlipidemicratfedwithepigallocatechin3gallatenanoparticles
AT xiaowenjun metagenomicsapproachtotheintestinalmicrobiomestructureandabundanceinhighfatdietinducedhyperlipidemicratfedwithepigallocatechin3gallatenanoparticles