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Genome-Wide Survey and Expression Analyses of Hexokinase Family in Poplar (Populus trichocarpa)
Hexokinase (HXK) family proteins exert critical roles in catalyzing hexose phosphorylation, sugar sensing, and modulation of plant growth and stress adaptation. Nevertheless, a large amount remains unknown about the molecular profile of HXK enzymes in Populus trichocarpa, a woody model tree species....
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9370503/ https://www.ncbi.nlm.nih.gov/pubmed/35956502 http://dx.doi.org/10.3390/plants11152025 |
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author | Han, Mei Xu, Xianglei Xiong, Yuan Wei, Haikun Yao, Kejun Huang, Tingting Long, Yingle Su, Tao |
author_facet | Han, Mei Xu, Xianglei Xiong, Yuan Wei, Haikun Yao, Kejun Huang, Tingting Long, Yingle Su, Tao |
author_sort | Han, Mei |
collection | PubMed |
description | Hexokinase (HXK) family proteins exert critical roles in catalyzing hexose phosphorylation, sugar sensing, and modulation of plant growth and stress adaptation. Nevertheless, a large amount remains unknown about the molecular profile of HXK enzymes in Populus trichocarpa, a woody model tree species. A genome-wide survey of HXK-encoding genes, including phylogenies, genomic structures, exon/intron organization, chromosomal distribution, and conserved features, was conducted, identifying six putative HXK isogenes (PtHXK1-6) in the Populus genome. The evolutionary tree demonstrated that 135 homologous HXKs between 17 plant species were categorized into four major subfamilies (type A, B, C, and D), clustering one plastidic (PtHXK3) and five mitochondrial PtHXKs grouped into type A and B, respectively. The in silico deduction prompted the presence of the conserved sugar-binding core (motif 4), phosphorylation sites (motif 2 and 3), and adenosine-binding domains (motif 7). The transcriptomic sequencing (RNA-seq) and the quantitative real-time PCR (qRT-PCR) assays revealed that three isogenes (PtHXK2, 3, and 6) were abundantly expressed in leaves, stems, and roots, while others appeared to be dominantly expressed in the reproductive tissues. Under the stress exposure, PtHXK2 and 6 displayed a significant induction upon the pathogenic fungi (Fusarium solani) infection and marked promotions by glucose feeding in roots. In contrast, the PtHXK3 and 6 are ABA-responsive genes, following a dose-dependent manner. The comprehensive analyses of the genomic patterns and expression profiling provide theoretical clues and lay a foundation for unraveling the physiological and signaling roles underlying the fine-tuned PtHXKs responding to diverse stressors. |
format | Online Article Text |
id | pubmed-9370503 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-93705032022-08-12 Genome-Wide Survey and Expression Analyses of Hexokinase Family in Poplar (Populus trichocarpa) Han, Mei Xu, Xianglei Xiong, Yuan Wei, Haikun Yao, Kejun Huang, Tingting Long, Yingle Su, Tao Plants (Basel) Article Hexokinase (HXK) family proteins exert critical roles in catalyzing hexose phosphorylation, sugar sensing, and modulation of plant growth and stress adaptation. Nevertheless, a large amount remains unknown about the molecular profile of HXK enzymes in Populus trichocarpa, a woody model tree species. A genome-wide survey of HXK-encoding genes, including phylogenies, genomic structures, exon/intron organization, chromosomal distribution, and conserved features, was conducted, identifying six putative HXK isogenes (PtHXK1-6) in the Populus genome. The evolutionary tree demonstrated that 135 homologous HXKs between 17 plant species were categorized into four major subfamilies (type A, B, C, and D), clustering one plastidic (PtHXK3) and five mitochondrial PtHXKs grouped into type A and B, respectively. The in silico deduction prompted the presence of the conserved sugar-binding core (motif 4), phosphorylation sites (motif 2 and 3), and adenosine-binding domains (motif 7). The transcriptomic sequencing (RNA-seq) and the quantitative real-time PCR (qRT-PCR) assays revealed that three isogenes (PtHXK2, 3, and 6) were abundantly expressed in leaves, stems, and roots, while others appeared to be dominantly expressed in the reproductive tissues. Under the stress exposure, PtHXK2 and 6 displayed a significant induction upon the pathogenic fungi (Fusarium solani) infection and marked promotions by glucose feeding in roots. In contrast, the PtHXK3 and 6 are ABA-responsive genes, following a dose-dependent manner. The comprehensive analyses of the genomic patterns and expression profiling provide theoretical clues and lay a foundation for unraveling the physiological and signaling roles underlying the fine-tuned PtHXKs responding to diverse stressors. MDPI 2022-08-03 /pmc/articles/PMC9370503/ /pubmed/35956502 http://dx.doi.org/10.3390/plants11152025 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Han, Mei Xu, Xianglei Xiong, Yuan Wei, Haikun Yao, Kejun Huang, Tingting Long, Yingle Su, Tao Genome-Wide Survey and Expression Analyses of Hexokinase Family in Poplar (Populus trichocarpa) |
title | Genome-Wide Survey and Expression Analyses of Hexokinase Family in Poplar (Populus trichocarpa) |
title_full | Genome-Wide Survey and Expression Analyses of Hexokinase Family in Poplar (Populus trichocarpa) |
title_fullStr | Genome-Wide Survey and Expression Analyses of Hexokinase Family in Poplar (Populus trichocarpa) |
title_full_unstemmed | Genome-Wide Survey and Expression Analyses of Hexokinase Family in Poplar (Populus trichocarpa) |
title_short | Genome-Wide Survey and Expression Analyses of Hexokinase Family in Poplar (Populus trichocarpa) |
title_sort | genome-wide survey and expression analyses of hexokinase family in poplar (populus trichocarpa) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9370503/ https://www.ncbi.nlm.nih.gov/pubmed/35956502 http://dx.doi.org/10.3390/plants11152025 |
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