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Evaluation of Dittrichia viscosa Aquaporin Nip1.1 Gene as Marker for Arsenic-Tolerant Plant Selection

Dittrichia viscosa (L.) Greuter is gaining attention for its high genetic plasticity and ability to adapt to adverse environmental conditions, including heavy metal and metalloid pollution. Uptake and translocation of cadmium, copper, iron, nickel, lead, and zinc to the shoots have been characterize...

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Autores principales: De Paolis, Angelo, De Caroli, Monica, Rojas, Makarena, Curci, Lorenzo Maria, Piro, Gabriella, Di Sansebastiano, Gian-Pietro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9370626/
https://www.ncbi.nlm.nih.gov/pubmed/35956446
http://dx.doi.org/10.3390/plants11151968
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author De Paolis, Angelo
De Caroli, Monica
Rojas, Makarena
Curci, Lorenzo Maria
Piro, Gabriella
Di Sansebastiano, Gian-Pietro
author_facet De Paolis, Angelo
De Caroli, Monica
Rojas, Makarena
Curci, Lorenzo Maria
Piro, Gabriella
Di Sansebastiano, Gian-Pietro
author_sort De Paolis, Angelo
collection PubMed
description Dittrichia viscosa (L.) Greuter is gaining attention for its high genetic plasticity and ability to adapt to adverse environmental conditions, including heavy metal and metalloid pollution. Uptake and translocation of cadmium, copper, iron, nickel, lead, and zinc to the shoots have been characterized, but its performance with arsenic is less known and sometimes contradictory. Tolerance to As is not related to a reduced uptake, but the null mutation of the aquaporin Nip1.1 gene in Arabidopsis makes the plant completely resistant to the metalloid. This aquaporin, localized in the endoplasmic reticulum, is responsible for arsenite and antimony (Sb) membrane permeation, but the uptake of arsenite occurs also in the null mutant, suggesting a more sophisticated action mechanism than direct uptake. In this study, the DvNip1 gene homologue is cloned and its expression profile in roots and shoots is characterized in different arsenic stress conditions. The use of clonal lines allowed to evidence that DvNip1.1 expression level is influenced by arsenic stress. The proportion of gene expression in roots and shoots can be used to generate an index that appears to be a promising putative selection marker to predict arsenic-resistant lines of Dittrichia viscosa plants.
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spelling pubmed-93706262022-08-12 Evaluation of Dittrichia viscosa Aquaporin Nip1.1 Gene as Marker for Arsenic-Tolerant Plant Selection De Paolis, Angelo De Caroli, Monica Rojas, Makarena Curci, Lorenzo Maria Piro, Gabriella Di Sansebastiano, Gian-Pietro Plants (Basel) Article Dittrichia viscosa (L.) Greuter is gaining attention for its high genetic plasticity and ability to adapt to adverse environmental conditions, including heavy metal and metalloid pollution. Uptake and translocation of cadmium, copper, iron, nickel, lead, and zinc to the shoots have been characterized, but its performance with arsenic is less known and sometimes contradictory. Tolerance to As is not related to a reduced uptake, but the null mutation of the aquaporin Nip1.1 gene in Arabidopsis makes the plant completely resistant to the metalloid. This aquaporin, localized in the endoplasmic reticulum, is responsible for arsenite and antimony (Sb) membrane permeation, but the uptake of arsenite occurs also in the null mutant, suggesting a more sophisticated action mechanism than direct uptake. In this study, the DvNip1 gene homologue is cloned and its expression profile in roots and shoots is characterized in different arsenic stress conditions. The use of clonal lines allowed to evidence that DvNip1.1 expression level is influenced by arsenic stress. The proportion of gene expression in roots and shoots can be used to generate an index that appears to be a promising putative selection marker to predict arsenic-resistant lines of Dittrichia viscosa plants. MDPI 2022-07-28 /pmc/articles/PMC9370626/ /pubmed/35956446 http://dx.doi.org/10.3390/plants11151968 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
De Paolis, Angelo
De Caroli, Monica
Rojas, Makarena
Curci, Lorenzo Maria
Piro, Gabriella
Di Sansebastiano, Gian-Pietro
Evaluation of Dittrichia viscosa Aquaporin Nip1.1 Gene as Marker for Arsenic-Tolerant Plant Selection
title Evaluation of Dittrichia viscosa Aquaporin Nip1.1 Gene as Marker for Arsenic-Tolerant Plant Selection
title_full Evaluation of Dittrichia viscosa Aquaporin Nip1.1 Gene as Marker for Arsenic-Tolerant Plant Selection
title_fullStr Evaluation of Dittrichia viscosa Aquaporin Nip1.1 Gene as Marker for Arsenic-Tolerant Plant Selection
title_full_unstemmed Evaluation of Dittrichia viscosa Aquaporin Nip1.1 Gene as Marker for Arsenic-Tolerant Plant Selection
title_short Evaluation of Dittrichia viscosa Aquaporin Nip1.1 Gene as Marker for Arsenic-Tolerant Plant Selection
title_sort evaluation of dittrichia viscosa aquaporin nip1.1 gene as marker for arsenic-tolerant plant selection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9370626/
https://www.ncbi.nlm.nih.gov/pubmed/35956446
http://dx.doi.org/10.3390/plants11151968
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