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Fe(3)O(4)-PAA–(HP-γ-CDs) Biocompatible Ferrimagnetic Nanoparticles for Increasing the Efficacy in Superparamagnetic Hyperthermia

In this paper, we present the obtaining of Fe(3)O(4)-PAA–(HP-γ-CDs) ferrimagnetic nanobioconjugates (PAA: polyacrylic acid, HP-γ-CDs: hydroxypropyl gamma-cyclodextrins) in a hybrid core-shell biostructure (core: inorganic Fe(3)O(4) nanoparticles, and shell: organic PAA–(HP-γ-CDs)) and their use in s...

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Autores principales: Caizer, Costica, Caizer, Isabela Simona, Racoviceanu, Roxana, Watz, Claudia Geanina, Mioc, Marius, Dehelean, Cristina Adriana, Bratu, Tiberiu, Soica, Codruța
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9370715/
https://www.ncbi.nlm.nih.gov/pubmed/35957011
http://dx.doi.org/10.3390/nano12152577
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author Caizer, Costica
Caizer, Isabela Simona
Racoviceanu, Roxana
Watz, Claudia Geanina
Mioc, Marius
Dehelean, Cristina Adriana
Bratu, Tiberiu
Soica, Codruța
author_facet Caizer, Costica
Caizer, Isabela Simona
Racoviceanu, Roxana
Watz, Claudia Geanina
Mioc, Marius
Dehelean, Cristina Adriana
Bratu, Tiberiu
Soica, Codruța
author_sort Caizer, Costica
collection PubMed
description In this paper, we present the obtaining of Fe(3)O(4)-PAA–(HP-γ-CDs) ferrimagnetic nanobioconjugates (PAA: polyacrylic acid, HP-γ-CDs: hydroxypropyl gamma-cyclodextrins) in a hybrid core-shell biostructure (core: inorganic Fe(3)O(4) nanoparticles, and shell: organic PAA–(HP-γ-CDs)) and their use in superparamagnetic hyperthermia without cellular toxicity and with increased efficacy for future alternative cancer therapy. In order to design the optimal experimental conditions for obtaining nanobioconjugates and then superparamagnetic hyperthermia (SPMHT), we used molecular docking simulation and computational assessment of the maximum specific loss power (SLP) that led to nanoparticles’ heating. The nanoparticles and nanobioconjugates obtained were studied and characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transformed-infrared spectroscopy (FT-IR), dynamic light scattering (DLS), and magnetic measurements (MMs). The cell viability of the nanoparticles and nanobioconjugates was assessed by means of the MTT assay using human immortalized keratinocytes (HaCaT) as an in vitro model. Superparamagnetic hyperthermia with nanoparticles and nanobioconjugates was obtained experimentally in a magnetic field of 15.92 kA/m and frequency of 312.2 kHz for the magnetic nanoparticle core with a (average) diameter of 15.8 nm, which resulted in the maximum hyperthermic effect that led to a temperature of ~42.5 °C necessary in the therapy of tumors in a short time so as not to affect healthy tissues. The biological screening of Fe(3)O(4)-PAA nanoparticles and PAA–(HP-γ-CDs) nanobioconjugates showed no cytotoxic effect on HaCaT cells for a time interval of 24 h, both under standard (37 °C) and hyperthermia conditions (42.5 °C). Thus, Fe(3)O(4)-PA–(HP-γ-CDs) ferrimagnetic nanobioconjugates can be used successfully in superparamagnetic hyperthermia without toxicity and with increased efficiency due to the small layer thickness of the PAA–(HP-γ-CDs) shell, which is suitable in this alternative therapeutic technique.
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spelling pubmed-93707152022-08-12 Fe(3)O(4)-PAA–(HP-γ-CDs) Biocompatible Ferrimagnetic Nanoparticles for Increasing the Efficacy in Superparamagnetic Hyperthermia Caizer, Costica Caizer, Isabela Simona Racoviceanu, Roxana Watz, Claudia Geanina Mioc, Marius Dehelean, Cristina Adriana Bratu, Tiberiu Soica, Codruța Nanomaterials (Basel) Article In this paper, we present the obtaining of Fe(3)O(4)-PAA–(HP-γ-CDs) ferrimagnetic nanobioconjugates (PAA: polyacrylic acid, HP-γ-CDs: hydroxypropyl gamma-cyclodextrins) in a hybrid core-shell biostructure (core: inorganic Fe(3)O(4) nanoparticles, and shell: organic PAA–(HP-γ-CDs)) and their use in superparamagnetic hyperthermia without cellular toxicity and with increased efficacy for future alternative cancer therapy. In order to design the optimal experimental conditions for obtaining nanobioconjugates and then superparamagnetic hyperthermia (SPMHT), we used molecular docking simulation and computational assessment of the maximum specific loss power (SLP) that led to nanoparticles’ heating. The nanoparticles and nanobioconjugates obtained were studied and characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transformed-infrared spectroscopy (FT-IR), dynamic light scattering (DLS), and magnetic measurements (MMs). The cell viability of the nanoparticles and nanobioconjugates was assessed by means of the MTT assay using human immortalized keratinocytes (HaCaT) as an in vitro model. Superparamagnetic hyperthermia with nanoparticles and nanobioconjugates was obtained experimentally in a magnetic field of 15.92 kA/m and frequency of 312.2 kHz for the magnetic nanoparticle core with a (average) diameter of 15.8 nm, which resulted in the maximum hyperthermic effect that led to a temperature of ~42.5 °C necessary in the therapy of tumors in a short time so as not to affect healthy tissues. The biological screening of Fe(3)O(4)-PAA nanoparticles and PAA–(HP-γ-CDs) nanobioconjugates showed no cytotoxic effect on HaCaT cells for a time interval of 24 h, both under standard (37 °C) and hyperthermia conditions (42.5 °C). Thus, Fe(3)O(4)-PA–(HP-γ-CDs) ferrimagnetic nanobioconjugates can be used successfully in superparamagnetic hyperthermia without toxicity and with increased efficiency due to the small layer thickness of the PAA–(HP-γ-CDs) shell, which is suitable in this alternative therapeutic technique. MDPI 2022-07-27 /pmc/articles/PMC9370715/ /pubmed/35957011 http://dx.doi.org/10.3390/nano12152577 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Caizer, Costica
Caizer, Isabela Simona
Racoviceanu, Roxana
Watz, Claudia Geanina
Mioc, Marius
Dehelean, Cristina Adriana
Bratu, Tiberiu
Soica, Codruța
Fe(3)O(4)-PAA–(HP-γ-CDs) Biocompatible Ferrimagnetic Nanoparticles for Increasing the Efficacy in Superparamagnetic Hyperthermia
title Fe(3)O(4)-PAA–(HP-γ-CDs) Biocompatible Ferrimagnetic Nanoparticles for Increasing the Efficacy in Superparamagnetic Hyperthermia
title_full Fe(3)O(4)-PAA–(HP-γ-CDs) Biocompatible Ferrimagnetic Nanoparticles for Increasing the Efficacy in Superparamagnetic Hyperthermia
title_fullStr Fe(3)O(4)-PAA–(HP-γ-CDs) Biocompatible Ferrimagnetic Nanoparticles for Increasing the Efficacy in Superparamagnetic Hyperthermia
title_full_unstemmed Fe(3)O(4)-PAA–(HP-γ-CDs) Biocompatible Ferrimagnetic Nanoparticles for Increasing the Efficacy in Superparamagnetic Hyperthermia
title_short Fe(3)O(4)-PAA–(HP-γ-CDs) Biocompatible Ferrimagnetic Nanoparticles for Increasing the Efficacy in Superparamagnetic Hyperthermia
title_sort fe(3)o(4)-paa–(hp-γ-cds) biocompatible ferrimagnetic nanoparticles for increasing the efficacy in superparamagnetic hyperthermia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9370715/
https://www.ncbi.nlm.nih.gov/pubmed/35957011
http://dx.doi.org/10.3390/nano12152577
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