Cargando…

Azadirachta indica A. Juss Fruit Mesocarp and Epicarp Extracts Induce Antimicrobial and Antiproliferative Effects against Prostate (PC-3), Breast (MCF-7), and Colorectal Adenocarcinoma (Caco-2) Cancer Cell Lines through Upregulation of Proapoptotic Genes

Effective alternative strategies and methodological approaches are critically necessary for cancer prevention and therapy. In this study, we investigated the antitumor potential of neem fruit mesocarp and epicarp extracts. The chemical composition of the derived extracts was characterized using GC–M...

Descripción completa

Detalles Bibliográficos
Autores principales: Ibrahim, Omer H. M., Mousa, Magdi A. A., Asiry, Khalid A., Alhakamy, Nabil A., Abo-Elyousr, Kamal A. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9370754/
https://www.ncbi.nlm.nih.gov/pubmed/35956468
http://dx.doi.org/10.3390/plants11151990
_version_ 1784766906663698432
author Ibrahim, Omer H. M.
Mousa, Magdi A. A.
Asiry, Khalid A.
Alhakamy, Nabil A.
Abo-Elyousr, Kamal A. M.
author_facet Ibrahim, Omer H. M.
Mousa, Magdi A. A.
Asiry, Khalid A.
Alhakamy, Nabil A.
Abo-Elyousr, Kamal A. M.
author_sort Ibrahim, Omer H. M.
collection PubMed
description Effective alternative strategies and methodological approaches are critically necessary for cancer prevention and therapy. In this study, we investigated the antitumor potential of neem fruit mesocarp and epicarp extracts. The chemical composition of the derived extracts was characterized using GC–MS. Data were collected on the antimicrobial activity of the extracts in addition to the cytotoxicity effect evaluated against PC-3, MCF-7, and Caco-2 cancer cell lines, compared with the normal Vero cells. Cell-cycle arrest, apoptosis, and expression of apoptosis-related genes were assessed on PC-3 cells. Both extracts had significant antiproliferative effects on all tested cell lines in a dose-dependent manner, with the mesocarp extract being more potent. Both extracts also showed high antibacterial and antifungal activities. These results were related to the chemical constituents of the extracts identified by the GC–MS analysis. The extract of neem fruit mesocarp caused cell-cycle arrest at G2/M phase of PC-3 cells. The cytotoxicity of neem mesocarp extract is strongly correlated with the induction of apoptosis, where it caused downregulation of the antiapoptotic BCL2 gene but upregulation of the proapoptotic P53 and BAX genes. This study showed that neem fruit extract is potential anticancer material in the future.
format Online
Article
Text
id pubmed-9370754
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-93707542022-08-12 Azadirachta indica A. Juss Fruit Mesocarp and Epicarp Extracts Induce Antimicrobial and Antiproliferative Effects against Prostate (PC-3), Breast (MCF-7), and Colorectal Adenocarcinoma (Caco-2) Cancer Cell Lines through Upregulation of Proapoptotic Genes Ibrahim, Omer H. M. Mousa, Magdi A. A. Asiry, Khalid A. Alhakamy, Nabil A. Abo-Elyousr, Kamal A. M. Plants (Basel) Article Effective alternative strategies and methodological approaches are critically necessary for cancer prevention and therapy. In this study, we investigated the antitumor potential of neem fruit mesocarp and epicarp extracts. The chemical composition of the derived extracts was characterized using GC–MS. Data were collected on the antimicrobial activity of the extracts in addition to the cytotoxicity effect evaluated against PC-3, MCF-7, and Caco-2 cancer cell lines, compared with the normal Vero cells. Cell-cycle arrest, apoptosis, and expression of apoptosis-related genes were assessed on PC-3 cells. Both extracts had significant antiproliferative effects on all tested cell lines in a dose-dependent manner, with the mesocarp extract being more potent. Both extracts also showed high antibacterial and antifungal activities. These results were related to the chemical constituents of the extracts identified by the GC–MS analysis. The extract of neem fruit mesocarp caused cell-cycle arrest at G2/M phase of PC-3 cells. The cytotoxicity of neem mesocarp extract is strongly correlated with the induction of apoptosis, where it caused downregulation of the antiapoptotic BCL2 gene but upregulation of the proapoptotic P53 and BAX genes. This study showed that neem fruit extract is potential anticancer material in the future. MDPI 2022-07-30 /pmc/articles/PMC9370754/ /pubmed/35956468 http://dx.doi.org/10.3390/plants11151990 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ibrahim, Omer H. M.
Mousa, Magdi A. A.
Asiry, Khalid A.
Alhakamy, Nabil A.
Abo-Elyousr, Kamal A. M.
Azadirachta indica A. Juss Fruit Mesocarp and Epicarp Extracts Induce Antimicrobial and Antiproliferative Effects against Prostate (PC-3), Breast (MCF-7), and Colorectal Adenocarcinoma (Caco-2) Cancer Cell Lines through Upregulation of Proapoptotic Genes
title Azadirachta indica A. Juss Fruit Mesocarp and Epicarp Extracts Induce Antimicrobial and Antiproliferative Effects against Prostate (PC-3), Breast (MCF-7), and Colorectal Adenocarcinoma (Caco-2) Cancer Cell Lines through Upregulation of Proapoptotic Genes
title_full Azadirachta indica A. Juss Fruit Mesocarp and Epicarp Extracts Induce Antimicrobial and Antiproliferative Effects against Prostate (PC-3), Breast (MCF-7), and Colorectal Adenocarcinoma (Caco-2) Cancer Cell Lines through Upregulation of Proapoptotic Genes
title_fullStr Azadirachta indica A. Juss Fruit Mesocarp and Epicarp Extracts Induce Antimicrobial and Antiproliferative Effects against Prostate (PC-3), Breast (MCF-7), and Colorectal Adenocarcinoma (Caco-2) Cancer Cell Lines through Upregulation of Proapoptotic Genes
title_full_unstemmed Azadirachta indica A. Juss Fruit Mesocarp and Epicarp Extracts Induce Antimicrobial and Antiproliferative Effects against Prostate (PC-3), Breast (MCF-7), and Colorectal Adenocarcinoma (Caco-2) Cancer Cell Lines through Upregulation of Proapoptotic Genes
title_short Azadirachta indica A. Juss Fruit Mesocarp and Epicarp Extracts Induce Antimicrobial and Antiproliferative Effects against Prostate (PC-3), Breast (MCF-7), and Colorectal Adenocarcinoma (Caco-2) Cancer Cell Lines through Upregulation of Proapoptotic Genes
title_sort azadirachta indica a. juss fruit mesocarp and epicarp extracts induce antimicrobial and antiproliferative effects against prostate (pc-3), breast (mcf-7), and colorectal adenocarcinoma (caco-2) cancer cell lines through upregulation of proapoptotic genes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9370754/
https://www.ncbi.nlm.nih.gov/pubmed/35956468
http://dx.doi.org/10.3390/plants11151990
work_keys_str_mv AT ibrahimomerhm azadirachtaindicaajussfruitmesocarpandepicarpextractsinduceantimicrobialandantiproliferativeeffectsagainstprostatepc3breastmcf7andcolorectaladenocarcinomacaco2cancercelllinesthroughupregulationofproapoptoticgenes
AT mousamagdiaa azadirachtaindicaajussfruitmesocarpandepicarpextractsinduceantimicrobialandantiproliferativeeffectsagainstprostatepc3breastmcf7andcolorectaladenocarcinomacaco2cancercelllinesthroughupregulationofproapoptoticgenes
AT asirykhalida azadirachtaindicaajussfruitmesocarpandepicarpextractsinduceantimicrobialandantiproliferativeeffectsagainstprostatepc3breastmcf7andcolorectaladenocarcinomacaco2cancercelllinesthroughupregulationofproapoptoticgenes
AT alhakamynabila azadirachtaindicaajussfruitmesocarpandepicarpextractsinduceantimicrobialandantiproliferativeeffectsagainstprostatepc3breastmcf7andcolorectaladenocarcinomacaco2cancercelllinesthroughupregulationofproapoptoticgenes
AT aboelyousrkamalam azadirachtaindicaajussfruitmesocarpandepicarpextractsinduceantimicrobialandantiproliferativeeffectsagainstprostatepc3breastmcf7andcolorectaladenocarcinomacaco2cancercelllinesthroughupregulationofproapoptoticgenes