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Biomolecular Corona Stability in Association with Plasma Cholesterol Level
Biomolecular corona is spontaneously formed on the surface of nanoparticles (NPs) when they are in contact with biological fluids. It plays an important role in the colloidal stability of NPs, which is of importance for most of their medical applications and toxicity assessment. While typical studie...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9370777/ https://www.ncbi.nlm.nih.gov/pubmed/35957093 http://dx.doi.org/10.3390/nano12152661 |
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author | Trinh, Duong N. Radlinskaite, Meda Cheeseman, Jack Kuhnle, Gunter Osborn, Helen M. I. Meleady, Paula Spencer, Daniel I. R. Monopoli, Marco P. |
author_facet | Trinh, Duong N. Radlinskaite, Meda Cheeseman, Jack Kuhnle, Gunter Osborn, Helen M. I. Meleady, Paula Spencer, Daniel I. R. Monopoli, Marco P. |
author_sort | Trinh, Duong N. |
collection | PubMed |
description | Biomolecular corona is spontaneously formed on the surface of nanoparticles (NPs) when they are in contact with biological fluids. It plays an important role in the colloidal stability of NPs, which is of importance for most of their medical applications and toxicity assessment. While typical studies use either blood plasma or serum from a pooled biobank, it is unclear whether differences in the media, such as cholesterol level or protein concentration, might affect the NP colloidal stability and corona composition. In this study, the silica corona was prepared at particularly low plasma concentrations (3%, v/v–1.98 mg/mL) to identify the critical roles of the protein mass/NP surface ratio and the level of plasma cholesterol on the corona protein pattern and particle stability. While depending on the plasma dilution factor, the corona protein composition could be controlled by keeping the protein/NP constant. The NP colloidal stability was found to strongly correlate with the level of cholesterol in human plasma, particularly due to the high enrichment of high-density lipoprotein (HDL) and low-density lipoprotein (LDL) in the corona. A cohort study on plasma samples from individuals with known cholesterol levels was performed to highlight that association, which could be relevant for all corona systems enriched with the LDL. |
format | Online Article Text |
id | pubmed-9370777 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-93707772022-08-12 Biomolecular Corona Stability in Association with Plasma Cholesterol Level Trinh, Duong N. Radlinskaite, Meda Cheeseman, Jack Kuhnle, Gunter Osborn, Helen M. I. Meleady, Paula Spencer, Daniel I. R. Monopoli, Marco P. Nanomaterials (Basel) Article Biomolecular corona is spontaneously formed on the surface of nanoparticles (NPs) when they are in contact with biological fluids. It plays an important role in the colloidal stability of NPs, which is of importance for most of their medical applications and toxicity assessment. While typical studies use either blood plasma or serum from a pooled biobank, it is unclear whether differences in the media, such as cholesterol level or protein concentration, might affect the NP colloidal stability and corona composition. In this study, the silica corona was prepared at particularly low plasma concentrations (3%, v/v–1.98 mg/mL) to identify the critical roles of the protein mass/NP surface ratio and the level of plasma cholesterol on the corona protein pattern and particle stability. While depending on the plasma dilution factor, the corona protein composition could be controlled by keeping the protein/NP constant. The NP colloidal stability was found to strongly correlate with the level of cholesterol in human plasma, particularly due to the high enrichment of high-density lipoprotein (HDL) and low-density lipoprotein (LDL) in the corona. A cohort study on plasma samples from individuals with known cholesterol levels was performed to highlight that association, which could be relevant for all corona systems enriched with the LDL. MDPI 2022-08-03 /pmc/articles/PMC9370777/ /pubmed/35957093 http://dx.doi.org/10.3390/nano12152661 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Trinh, Duong N. Radlinskaite, Meda Cheeseman, Jack Kuhnle, Gunter Osborn, Helen M. I. Meleady, Paula Spencer, Daniel I. R. Monopoli, Marco P. Biomolecular Corona Stability in Association with Plasma Cholesterol Level |
title | Biomolecular Corona Stability in Association with Plasma Cholesterol Level |
title_full | Biomolecular Corona Stability in Association with Plasma Cholesterol Level |
title_fullStr | Biomolecular Corona Stability in Association with Plasma Cholesterol Level |
title_full_unstemmed | Biomolecular Corona Stability in Association with Plasma Cholesterol Level |
title_short | Biomolecular Corona Stability in Association with Plasma Cholesterol Level |
title_sort | biomolecular corona stability in association with plasma cholesterol level |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9370777/ https://www.ncbi.nlm.nih.gov/pubmed/35957093 http://dx.doi.org/10.3390/nano12152661 |
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