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Biomolecular Corona Stability in Association with Plasma Cholesterol Level

Biomolecular corona is spontaneously formed on the surface of nanoparticles (NPs) when they are in contact with biological fluids. It plays an important role in the colloidal stability of NPs, which is of importance for most of their medical applications and toxicity assessment. While typical studie...

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Autores principales: Trinh, Duong N., Radlinskaite, Meda, Cheeseman, Jack, Kuhnle, Gunter, Osborn, Helen M. I., Meleady, Paula, Spencer, Daniel I. R., Monopoli, Marco P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9370777/
https://www.ncbi.nlm.nih.gov/pubmed/35957093
http://dx.doi.org/10.3390/nano12152661
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author Trinh, Duong N.
Radlinskaite, Meda
Cheeseman, Jack
Kuhnle, Gunter
Osborn, Helen M. I.
Meleady, Paula
Spencer, Daniel I. R.
Monopoli, Marco P.
author_facet Trinh, Duong N.
Radlinskaite, Meda
Cheeseman, Jack
Kuhnle, Gunter
Osborn, Helen M. I.
Meleady, Paula
Spencer, Daniel I. R.
Monopoli, Marco P.
author_sort Trinh, Duong N.
collection PubMed
description Biomolecular corona is spontaneously formed on the surface of nanoparticles (NPs) when they are in contact with biological fluids. It plays an important role in the colloidal stability of NPs, which is of importance for most of their medical applications and toxicity assessment. While typical studies use either blood plasma or serum from a pooled biobank, it is unclear whether differences in the media, such as cholesterol level or protein concentration, might affect the NP colloidal stability and corona composition. In this study, the silica corona was prepared at particularly low plasma concentrations (3%, v/v–1.98 mg/mL) to identify the critical roles of the protein mass/NP surface ratio and the level of plasma cholesterol on the corona protein pattern and particle stability. While depending on the plasma dilution factor, the corona protein composition could be controlled by keeping the protein/NP constant. The NP colloidal stability was found to strongly correlate with the level of cholesterol in human plasma, particularly due to the high enrichment of high-density lipoprotein (HDL) and low-density lipoprotein (LDL) in the corona. A cohort study on plasma samples from individuals with known cholesterol levels was performed to highlight that association, which could be relevant for all corona systems enriched with the LDL.
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spelling pubmed-93707772022-08-12 Biomolecular Corona Stability in Association with Plasma Cholesterol Level Trinh, Duong N. Radlinskaite, Meda Cheeseman, Jack Kuhnle, Gunter Osborn, Helen M. I. Meleady, Paula Spencer, Daniel I. R. Monopoli, Marco P. Nanomaterials (Basel) Article Biomolecular corona is spontaneously formed on the surface of nanoparticles (NPs) when they are in contact with biological fluids. It plays an important role in the colloidal stability of NPs, which is of importance for most of their medical applications and toxicity assessment. While typical studies use either blood plasma or serum from a pooled biobank, it is unclear whether differences in the media, such as cholesterol level or protein concentration, might affect the NP colloidal stability and corona composition. In this study, the silica corona was prepared at particularly low plasma concentrations (3%, v/v–1.98 mg/mL) to identify the critical roles of the protein mass/NP surface ratio and the level of plasma cholesterol on the corona protein pattern and particle stability. While depending on the plasma dilution factor, the corona protein composition could be controlled by keeping the protein/NP constant. The NP colloidal stability was found to strongly correlate with the level of cholesterol in human plasma, particularly due to the high enrichment of high-density lipoprotein (HDL) and low-density lipoprotein (LDL) in the corona. A cohort study on plasma samples from individuals with known cholesterol levels was performed to highlight that association, which could be relevant for all corona systems enriched with the LDL. MDPI 2022-08-03 /pmc/articles/PMC9370777/ /pubmed/35957093 http://dx.doi.org/10.3390/nano12152661 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Trinh, Duong N.
Radlinskaite, Meda
Cheeseman, Jack
Kuhnle, Gunter
Osborn, Helen M. I.
Meleady, Paula
Spencer, Daniel I. R.
Monopoli, Marco P.
Biomolecular Corona Stability in Association with Plasma Cholesterol Level
title Biomolecular Corona Stability in Association with Plasma Cholesterol Level
title_full Biomolecular Corona Stability in Association with Plasma Cholesterol Level
title_fullStr Biomolecular Corona Stability in Association with Plasma Cholesterol Level
title_full_unstemmed Biomolecular Corona Stability in Association with Plasma Cholesterol Level
title_short Biomolecular Corona Stability in Association with Plasma Cholesterol Level
title_sort biomolecular corona stability in association with plasma cholesterol level
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9370777/
https://www.ncbi.nlm.nih.gov/pubmed/35957093
http://dx.doi.org/10.3390/nano12152661
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