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AtZAT4, a C(2)H(2)-Type Zinc Finger Transcription Factor from Arabidopsis thaliana, Is Involved in Pollen and Seed Development
Pollen plays an essential role in plant fertility by delivering the male gametes to the embryo sac before double fertilization. In several plant species, including Arabidopsis, C(2)H(2)-type zinc-finger transcription factors (TFs) have been involved in different stages of pollen development and matu...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9370812/ https://www.ncbi.nlm.nih.gov/pubmed/35956451 http://dx.doi.org/10.3390/plants11151974 |
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author | Puentes-Romero, A. Carolina González, Sebastián A. González-Villanueva, Enrique Figueroa, Carlos R. Ruiz-Lara, Simón |
author_facet | Puentes-Romero, A. Carolina González, Sebastián A. González-Villanueva, Enrique Figueroa, Carlos R. Ruiz-Lara, Simón |
author_sort | Puentes-Romero, A. Carolina |
collection | PubMed |
description | Pollen plays an essential role in plant fertility by delivering the male gametes to the embryo sac before double fertilization. In several plant species, including Arabidopsis, C(2)H(2)-type zinc-finger transcription factors (TFs) have been involved in different stages of pollen development and maturation. ZINC FINGER of Arabidopsis thaliana 4 (AtZAT4) is homologous to such TFs and subcellular localization analysis has revealed that AtZAT4 is located in the nucleus. Moreover, analysis of AtZAT4 expression revealed strong levels of it in flowers and siliques, suggesting a role of the encoded protein in the regulation of genes that are associated with reproductive development. We characterized a T-DNA insertional heterozygous mutant Atzat4 (+/−). The relative gene expression analysis of Atzat4 (+/−) showed significant transcript reductions in flowers and siliques. Furthermore, the Atzat4 (+/−) phenotypic characterization revealed defects in the male germline, showing a reduction in pollen tube germination and elongation. Atzat4 (+/−) presented reduced fertility, characterized by a smaller silique size compared to the wild type (WT), and a lower number of seeds per silique. Additionally, seeds displayed lower viability and germination. Altogether, our data suggest a role for AtZAT4 in fertilization and seed viability, through the regulation of gene expression associated with reproductive development. |
format | Online Article Text |
id | pubmed-9370812 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-93708122022-08-12 AtZAT4, a C(2)H(2)-Type Zinc Finger Transcription Factor from Arabidopsis thaliana, Is Involved in Pollen and Seed Development Puentes-Romero, A. Carolina González, Sebastián A. González-Villanueva, Enrique Figueroa, Carlos R. Ruiz-Lara, Simón Plants (Basel) Article Pollen plays an essential role in plant fertility by delivering the male gametes to the embryo sac before double fertilization. In several plant species, including Arabidopsis, C(2)H(2)-type zinc-finger transcription factors (TFs) have been involved in different stages of pollen development and maturation. ZINC FINGER of Arabidopsis thaliana 4 (AtZAT4) is homologous to such TFs and subcellular localization analysis has revealed that AtZAT4 is located in the nucleus. Moreover, analysis of AtZAT4 expression revealed strong levels of it in flowers and siliques, suggesting a role of the encoded protein in the regulation of genes that are associated with reproductive development. We characterized a T-DNA insertional heterozygous mutant Atzat4 (+/−). The relative gene expression analysis of Atzat4 (+/−) showed significant transcript reductions in flowers and siliques. Furthermore, the Atzat4 (+/−) phenotypic characterization revealed defects in the male germline, showing a reduction in pollen tube germination and elongation. Atzat4 (+/−) presented reduced fertility, characterized by a smaller silique size compared to the wild type (WT), and a lower number of seeds per silique. Additionally, seeds displayed lower viability and germination. Altogether, our data suggest a role for AtZAT4 in fertilization and seed viability, through the regulation of gene expression associated with reproductive development. MDPI 2022-07-29 /pmc/articles/PMC9370812/ /pubmed/35956451 http://dx.doi.org/10.3390/plants11151974 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Puentes-Romero, A. Carolina González, Sebastián A. González-Villanueva, Enrique Figueroa, Carlos R. Ruiz-Lara, Simón AtZAT4, a C(2)H(2)-Type Zinc Finger Transcription Factor from Arabidopsis thaliana, Is Involved in Pollen and Seed Development |
title | AtZAT4, a C(2)H(2)-Type Zinc Finger Transcription Factor from Arabidopsis thaliana, Is Involved in Pollen and Seed Development |
title_full | AtZAT4, a C(2)H(2)-Type Zinc Finger Transcription Factor from Arabidopsis thaliana, Is Involved in Pollen and Seed Development |
title_fullStr | AtZAT4, a C(2)H(2)-Type Zinc Finger Transcription Factor from Arabidopsis thaliana, Is Involved in Pollen and Seed Development |
title_full_unstemmed | AtZAT4, a C(2)H(2)-Type Zinc Finger Transcription Factor from Arabidopsis thaliana, Is Involved in Pollen and Seed Development |
title_short | AtZAT4, a C(2)H(2)-Type Zinc Finger Transcription Factor from Arabidopsis thaliana, Is Involved in Pollen and Seed Development |
title_sort | atzat4, a c(2)h(2)-type zinc finger transcription factor from arabidopsis thaliana, is involved in pollen and seed development |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9370812/ https://www.ncbi.nlm.nih.gov/pubmed/35956451 http://dx.doi.org/10.3390/plants11151974 |
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