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Electrocrystallization of Calcium Oxalate on Electrospun PCL Fibers Loaded with Phytic Acid as a Template

Crystallization occurs widely in living organisms where different organs could associate with the calcification process, such as the formation of calcium oxalate (CaOx) calculi in the urinary tract. However, the pathogenesis and the role of an inhibitor in the pathological processes involved in urol...

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Autores principales: Arce, Tatiana Zegers, Yazdani-Pedram, Mehrdad, Neira-Carrillo, Andrónico
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9371010/
https://www.ncbi.nlm.nih.gov/pubmed/35956705
http://dx.doi.org/10.3390/polym14153190
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author Arce, Tatiana Zegers
Yazdani-Pedram, Mehrdad
Neira-Carrillo, Andrónico
author_facet Arce, Tatiana Zegers
Yazdani-Pedram, Mehrdad
Neira-Carrillo, Andrónico
author_sort Arce, Tatiana Zegers
collection PubMed
description Crystallization occurs widely in living organisms where different organs could associate with the calcification process, such as the formation of calcium oxalate (CaOx) calculi in the urinary tract. However, the pathogenesis and the role of an inhibitor in the pathological processes involved in urolithiasis is poorly understood. Therefore, the use of phytic acid (PA) as an inhibitor for the organic fibrillar matrix is a novel approach to inhibit the formation of pathological CaOx crystals. Herein, electrospun polymer fiber meshes of polycaprolactone (PCL) with random (R) and aligned (A) fiber orientations containing PA were prepared by electrospinning, and their role as a 3D organic template in in vitro CaOx crystallization was investigated. CaOx crystals were generated on conductive tin indium oxide (ITO)-modified glass with R-PCL and A-PCL fibers in the presence of PA through an electrocrystallization (EC) procedure. This study provides a simple electrochemical approach to evaluate the role of PA as an inhibitor in the nucleation of pathological CaOx crystals. The resulting CaOx crystals were analyzed by chrono-potentiometry, optical microscopy (OM), scanning electron microscopy (SEM), and X-ray diffraction (XRD). We found that PA and the fiber orientations are key factors in the nucleation and crystal growth of CaOx, achieving the stabilization of healthy CaOx crystal and the inhibition of the pathological crystal form.
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spelling pubmed-93710102022-08-12 Electrocrystallization of Calcium Oxalate on Electrospun PCL Fibers Loaded with Phytic Acid as a Template Arce, Tatiana Zegers Yazdani-Pedram, Mehrdad Neira-Carrillo, Andrónico Polymers (Basel) Article Crystallization occurs widely in living organisms where different organs could associate with the calcification process, such as the formation of calcium oxalate (CaOx) calculi in the urinary tract. However, the pathogenesis and the role of an inhibitor in the pathological processes involved in urolithiasis is poorly understood. Therefore, the use of phytic acid (PA) as an inhibitor for the organic fibrillar matrix is a novel approach to inhibit the formation of pathological CaOx crystals. Herein, electrospun polymer fiber meshes of polycaprolactone (PCL) with random (R) and aligned (A) fiber orientations containing PA were prepared by electrospinning, and their role as a 3D organic template in in vitro CaOx crystallization was investigated. CaOx crystals were generated on conductive tin indium oxide (ITO)-modified glass with R-PCL and A-PCL fibers in the presence of PA through an electrocrystallization (EC) procedure. This study provides a simple electrochemical approach to evaluate the role of PA as an inhibitor in the nucleation of pathological CaOx crystals. The resulting CaOx crystals were analyzed by chrono-potentiometry, optical microscopy (OM), scanning electron microscopy (SEM), and X-ray diffraction (XRD). We found that PA and the fiber orientations are key factors in the nucleation and crystal growth of CaOx, achieving the stabilization of healthy CaOx crystal and the inhibition of the pathological crystal form. MDPI 2022-08-05 /pmc/articles/PMC9371010/ /pubmed/35956705 http://dx.doi.org/10.3390/polym14153190 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Arce, Tatiana Zegers
Yazdani-Pedram, Mehrdad
Neira-Carrillo, Andrónico
Electrocrystallization of Calcium Oxalate on Electrospun PCL Fibers Loaded with Phytic Acid as a Template
title Electrocrystallization of Calcium Oxalate on Electrospun PCL Fibers Loaded with Phytic Acid as a Template
title_full Electrocrystallization of Calcium Oxalate on Electrospun PCL Fibers Loaded with Phytic Acid as a Template
title_fullStr Electrocrystallization of Calcium Oxalate on Electrospun PCL Fibers Loaded with Phytic Acid as a Template
title_full_unstemmed Electrocrystallization of Calcium Oxalate on Electrospun PCL Fibers Loaded with Phytic Acid as a Template
title_short Electrocrystallization of Calcium Oxalate on Electrospun PCL Fibers Loaded with Phytic Acid as a Template
title_sort electrocrystallization of calcium oxalate on electrospun pcl fibers loaded with phytic acid as a template
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9371010/
https://www.ncbi.nlm.nih.gov/pubmed/35956705
http://dx.doi.org/10.3390/polym14153190
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AT neiracarrilloandronico electrocrystallizationofcalciumoxalateonelectrospunpclfibersloadedwithphyticacidasatemplate