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In Vivo Multimodal Imaging of Stem Cells Using Nanohybrid Particles Incorporating Quantum Dots and Magnetic Nanoparticles

The diagnosis of the dynamics, accumulation, and engraftment of transplanted stem cells in vivo is essential for ensuring the safety and the maximum therapeutic effect of regenerative medicine. However, in vivo imaging technologies for detecting transplanted stem cells are not sufficient at present....

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Autores principales: Yamada, Shota, Yukawa, Hiroshi, Yamada, Kaori, Murata, Yuki, Jo, Jun-ichiro, Yamamoto, Masaya, Sugawara-Narutaki, Ayae, Tabata, Yasuhiko, Baba, Yoshinobu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9371134/
https://www.ncbi.nlm.nih.gov/pubmed/35957262
http://dx.doi.org/10.3390/s22155705
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author Yamada, Shota
Yukawa, Hiroshi
Yamada, Kaori
Murata, Yuki
Jo, Jun-ichiro
Yamamoto, Masaya
Sugawara-Narutaki, Ayae
Tabata, Yasuhiko
Baba, Yoshinobu
author_facet Yamada, Shota
Yukawa, Hiroshi
Yamada, Kaori
Murata, Yuki
Jo, Jun-ichiro
Yamamoto, Masaya
Sugawara-Narutaki, Ayae
Tabata, Yasuhiko
Baba, Yoshinobu
author_sort Yamada, Shota
collection PubMed
description The diagnosis of the dynamics, accumulation, and engraftment of transplanted stem cells in vivo is essential for ensuring the safety and the maximum therapeutic effect of regenerative medicine. However, in vivo imaging technologies for detecting transplanted stem cells are not sufficient at present. We developed nanohybrid particles composed of dendron-baring lipids having two unsaturated bonds (DLU2) molecules, quantum dots (QDs), and magnetic nanoparticles in order to diagnose the dynamics, accumulation, and engraftment of transplanted stem cells, and then addressed the labeling and in vivo fluorescence and magnetic resonance (MR) imaging of stem cells using the nanohybrid particles (DLU2-NPs). Five kinds of DLU2-NPs (DLU2-NPs-1-5) composed of different concentrations of DLU2 molecules, QDs525, QDs605, QDs705, and ATDM were prepared. Adipose tissue-derived stem cells (ASCs) were labeled with DLU2-NPs for 4 h incubation, no cytotoxicity or marked effect on the proliferation ability was observed in ASCs labeled with DLU2-NPs (640- or 320-fold diluted). ASCs labeled with DLU2-NPs (640-fold diluted) were transplanted subcutaneously onto the backs of mice, and the labeled ASCs could be imaged with good contrast using in vivo fluorescence and an MR imaging system. DLU2-NPs may be useful for in vivo multimodal imaging of transplanted stem cells.
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spelling pubmed-93711342022-08-12 In Vivo Multimodal Imaging of Stem Cells Using Nanohybrid Particles Incorporating Quantum Dots and Magnetic Nanoparticles Yamada, Shota Yukawa, Hiroshi Yamada, Kaori Murata, Yuki Jo, Jun-ichiro Yamamoto, Masaya Sugawara-Narutaki, Ayae Tabata, Yasuhiko Baba, Yoshinobu Sensors (Basel) Article The diagnosis of the dynamics, accumulation, and engraftment of transplanted stem cells in vivo is essential for ensuring the safety and the maximum therapeutic effect of regenerative medicine. However, in vivo imaging technologies for detecting transplanted stem cells are not sufficient at present. We developed nanohybrid particles composed of dendron-baring lipids having two unsaturated bonds (DLU2) molecules, quantum dots (QDs), and magnetic nanoparticles in order to diagnose the dynamics, accumulation, and engraftment of transplanted stem cells, and then addressed the labeling and in vivo fluorescence and magnetic resonance (MR) imaging of stem cells using the nanohybrid particles (DLU2-NPs). Five kinds of DLU2-NPs (DLU2-NPs-1-5) composed of different concentrations of DLU2 molecules, QDs525, QDs605, QDs705, and ATDM were prepared. Adipose tissue-derived stem cells (ASCs) were labeled with DLU2-NPs for 4 h incubation, no cytotoxicity or marked effect on the proliferation ability was observed in ASCs labeled with DLU2-NPs (640- or 320-fold diluted). ASCs labeled with DLU2-NPs (640-fold diluted) were transplanted subcutaneously onto the backs of mice, and the labeled ASCs could be imaged with good contrast using in vivo fluorescence and an MR imaging system. DLU2-NPs may be useful for in vivo multimodal imaging of transplanted stem cells. MDPI 2022-07-30 /pmc/articles/PMC9371134/ /pubmed/35957262 http://dx.doi.org/10.3390/s22155705 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yamada, Shota
Yukawa, Hiroshi
Yamada, Kaori
Murata, Yuki
Jo, Jun-ichiro
Yamamoto, Masaya
Sugawara-Narutaki, Ayae
Tabata, Yasuhiko
Baba, Yoshinobu
In Vivo Multimodal Imaging of Stem Cells Using Nanohybrid Particles Incorporating Quantum Dots and Magnetic Nanoparticles
title In Vivo Multimodal Imaging of Stem Cells Using Nanohybrid Particles Incorporating Quantum Dots and Magnetic Nanoparticles
title_full In Vivo Multimodal Imaging of Stem Cells Using Nanohybrid Particles Incorporating Quantum Dots and Magnetic Nanoparticles
title_fullStr In Vivo Multimodal Imaging of Stem Cells Using Nanohybrid Particles Incorporating Quantum Dots and Magnetic Nanoparticles
title_full_unstemmed In Vivo Multimodal Imaging of Stem Cells Using Nanohybrid Particles Incorporating Quantum Dots and Magnetic Nanoparticles
title_short In Vivo Multimodal Imaging of Stem Cells Using Nanohybrid Particles Incorporating Quantum Dots and Magnetic Nanoparticles
title_sort in vivo multimodal imaging of stem cells using nanohybrid particles incorporating quantum dots and magnetic nanoparticles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9371134/
https://www.ncbi.nlm.nih.gov/pubmed/35957262
http://dx.doi.org/10.3390/s22155705
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