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Planar Junctionless Field-Effect Transistor for Detecting Biomolecular Interactions
Label-free field-effect transistor-based immunosensors are promising candidates for proteomics and peptidomics-based diagnostics and therapeutics due to their high multiplexing capability, fast response time, and ability to increase the sensor sensitivity due to the short length of peptides. In this...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9371156/ https://www.ncbi.nlm.nih.gov/pubmed/35957340 http://dx.doi.org/10.3390/s22155783 |
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author | Shukla, Rajendra P. Bomer, J. G. Wijnperle, Daniel Kumar, Naveen Georgiev, Vihar P. Singh, Aruna Chandra Krishnamoorthy, Sivashankar Pascual García, César Pud, Sergii Olthuis, Wouter |
author_facet | Shukla, Rajendra P. Bomer, J. G. Wijnperle, Daniel Kumar, Naveen Georgiev, Vihar P. Singh, Aruna Chandra Krishnamoorthy, Sivashankar Pascual García, César Pud, Sergii Olthuis, Wouter |
author_sort | Shukla, Rajendra P. |
collection | PubMed |
description | Label-free field-effect transistor-based immunosensors are promising candidates for proteomics and peptidomics-based diagnostics and therapeutics due to their high multiplexing capability, fast response time, and ability to increase the sensor sensitivity due to the short length of peptides. In this work, planar junctionless field-effect transistor sensors (FETs) were fabricated and characterized for pH sensing. The device with SiO(2) gate oxide has shown voltage sensitivity of 41.8 ± 1.4, 39.9 ± 1.4, 39.0 ± 1.1, and 37.6 ± 1.0 mV/pH for constant drain currents of 5, 10, 20, and 50 nA, respectively, with a drain to source voltage of 0.05 V. The drift analysis shows a stability over time of −18 nA/h (pH 7.75), −3.5 nA/h (pH 6.84), −0.5 nA/h (pH 4.91), 0.5 nA/h (pH 3.43), corresponding to a pH drift of −0.45, −0.09, −0.01, and 0.01 per h. Theoretical modeling and simulation resulted in a mean value of the surface states of 3.8 × 10(15)/cm(2) with a standard deviation of 3.6 × 10(15)/cm(2). We have experimentally verified the number of surface sites due to APTES, peptide, and protein immobilization, which is in line with the theoretical calculations for FETs to be used for detecting peptide-protein interactions for future applications. |
format | Online Article Text |
id | pubmed-9371156 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-93711562022-08-12 Planar Junctionless Field-Effect Transistor for Detecting Biomolecular Interactions Shukla, Rajendra P. Bomer, J. G. Wijnperle, Daniel Kumar, Naveen Georgiev, Vihar P. Singh, Aruna Chandra Krishnamoorthy, Sivashankar Pascual García, César Pud, Sergii Olthuis, Wouter Sensors (Basel) Article Label-free field-effect transistor-based immunosensors are promising candidates for proteomics and peptidomics-based diagnostics and therapeutics due to their high multiplexing capability, fast response time, and ability to increase the sensor sensitivity due to the short length of peptides. In this work, planar junctionless field-effect transistor sensors (FETs) were fabricated and characterized for pH sensing. The device with SiO(2) gate oxide has shown voltage sensitivity of 41.8 ± 1.4, 39.9 ± 1.4, 39.0 ± 1.1, and 37.6 ± 1.0 mV/pH for constant drain currents of 5, 10, 20, and 50 nA, respectively, with a drain to source voltage of 0.05 V. The drift analysis shows a stability over time of −18 nA/h (pH 7.75), −3.5 nA/h (pH 6.84), −0.5 nA/h (pH 4.91), 0.5 nA/h (pH 3.43), corresponding to a pH drift of −0.45, −0.09, −0.01, and 0.01 per h. Theoretical modeling and simulation resulted in a mean value of the surface states of 3.8 × 10(15)/cm(2) with a standard deviation of 3.6 × 10(15)/cm(2). We have experimentally verified the number of surface sites due to APTES, peptide, and protein immobilization, which is in line with the theoretical calculations for FETs to be used for detecting peptide-protein interactions for future applications. MDPI 2022-08-02 /pmc/articles/PMC9371156/ /pubmed/35957340 http://dx.doi.org/10.3390/s22155783 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Shukla, Rajendra P. Bomer, J. G. Wijnperle, Daniel Kumar, Naveen Georgiev, Vihar P. Singh, Aruna Chandra Krishnamoorthy, Sivashankar Pascual García, César Pud, Sergii Olthuis, Wouter Planar Junctionless Field-Effect Transistor for Detecting Biomolecular Interactions |
title | Planar Junctionless Field-Effect Transistor for Detecting Biomolecular Interactions |
title_full | Planar Junctionless Field-Effect Transistor for Detecting Biomolecular Interactions |
title_fullStr | Planar Junctionless Field-Effect Transistor for Detecting Biomolecular Interactions |
title_full_unstemmed | Planar Junctionless Field-Effect Transistor for Detecting Biomolecular Interactions |
title_short | Planar Junctionless Field-Effect Transistor for Detecting Biomolecular Interactions |
title_sort | planar junctionless field-effect transistor for detecting biomolecular interactions |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9371156/ https://www.ncbi.nlm.nih.gov/pubmed/35957340 http://dx.doi.org/10.3390/s22155783 |
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