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A simple scoring of beam walking performance after spinal cord injury in mice

Precise evaluation of motor functions using simple and reproducible tests for mouse models of spinal cord injury (SCI) are required. Overground walking of SCI mice has been tested by Basso Mouse Scale for locomotion (BMS). In contrast, only a few works quantify walking performances of SCI mice on na...

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Autores principales: Ito, Shunsuke, Kakuta, Yohei, Yoshida, Kosuke, Shirota, Yuma, Mieda, Tokue, Iizuka, Yoichi, Chikuda, Hirotaka, Iizuka, Haku, Nakamura, Kazuhiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9371269/
https://www.ncbi.nlm.nih.gov/pubmed/35951582
http://dx.doi.org/10.1371/journal.pone.0272233
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author Ito, Shunsuke
Kakuta, Yohei
Yoshida, Kosuke
Shirota, Yuma
Mieda, Tokue
Iizuka, Yoichi
Chikuda, Hirotaka
Iizuka, Haku
Nakamura, Kazuhiro
author_facet Ito, Shunsuke
Kakuta, Yohei
Yoshida, Kosuke
Shirota, Yuma
Mieda, Tokue
Iizuka, Yoichi
Chikuda, Hirotaka
Iizuka, Haku
Nakamura, Kazuhiro
author_sort Ito, Shunsuke
collection PubMed
description Precise evaluation of motor functions using simple and reproducible tests for mouse models of spinal cord injury (SCI) are required. Overground walking of SCI mice has been tested by Basso Mouse Scale for locomotion (BMS). In contrast, only a few works quantify walking performances of SCI mice on narrow beams, a different task. Here, we established a novel scoring system using a single beam walking apparatus for SCI mice. The scoring system uses binary judgments of values such as retention, moving forward and reaching the goal on a beam for rating. In addition, high score was given to SCI mouse when the mouse efficiently used hindlimbs for locomotion on the beam. A high rate of concordance of the score derived from positions of hindlimbs between two observers was obtained. Mice displayed the lowest total score on the beam immediately after the SCI, then the score gradually increased like time course of BMS score. Furthermore, the total scores reflected gradation of severity of SCI in 2 strains of mice. The beam walking score proved to be strongly correlated with that of BMS score, indicating that performances between overground walking and beam walking are partly correlated in SCI mice. Collectively, the novel scoring system offers an opportunity to easily evaluate motor performances of mice with SCI.
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spelling pubmed-93712692022-08-12 A simple scoring of beam walking performance after spinal cord injury in mice Ito, Shunsuke Kakuta, Yohei Yoshida, Kosuke Shirota, Yuma Mieda, Tokue Iizuka, Yoichi Chikuda, Hirotaka Iizuka, Haku Nakamura, Kazuhiro PLoS One Research Article Precise evaluation of motor functions using simple and reproducible tests for mouse models of spinal cord injury (SCI) are required. Overground walking of SCI mice has been tested by Basso Mouse Scale for locomotion (BMS). In contrast, only a few works quantify walking performances of SCI mice on narrow beams, a different task. Here, we established a novel scoring system using a single beam walking apparatus for SCI mice. The scoring system uses binary judgments of values such as retention, moving forward and reaching the goal on a beam for rating. In addition, high score was given to SCI mouse when the mouse efficiently used hindlimbs for locomotion on the beam. A high rate of concordance of the score derived from positions of hindlimbs between two observers was obtained. Mice displayed the lowest total score on the beam immediately after the SCI, then the score gradually increased like time course of BMS score. Furthermore, the total scores reflected gradation of severity of SCI in 2 strains of mice. The beam walking score proved to be strongly correlated with that of BMS score, indicating that performances between overground walking and beam walking are partly correlated in SCI mice. Collectively, the novel scoring system offers an opportunity to easily evaluate motor performances of mice with SCI. Public Library of Science 2022-08-11 /pmc/articles/PMC9371269/ /pubmed/35951582 http://dx.doi.org/10.1371/journal.pone.0272233 Text en © 2022 Ito et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Ito, Shunsuke
Kakuta, Yohei
Yoshida, Kosuke
Shirota, Yuma
Mieda, Tokue
Iizuka, Yoichi
Chikuda, Hirotaka
Iizuka, Haku
Nakamura, Kazuhiro
A simple scoring of beam walking performance after spinal cord injury in mice
title A simple scoring of beam walking performance after spinal cord injury in mice
title_full A simple scoring of beam walking performance after spinal cord injury in mice
title_fullStr A simple scoring of beam walking performance after spinal cord injury in mice
title_full_unstemmed A simple scoring of beam walking performance after spinal cord injury in mice
title_short A simple scoring of beam walking performance after spinal cord injury in mice
title_sort simple scoring of beam walking performance after spinal cord injury in mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9371269/
https://www.ncbi.nlm.nih.gov/pubmed/35951582
http://dx.doi.org/10.1371/journal.pone.0272233
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