The Expression of the Long Noncoding RNA AFAP1-AS1 in Laryngeal Carcinoma Affects the Proliferation, Invasion, Migration, and Apoptosis of TU212 Cell Line

BACKGROUND: lncRNA AFAP1-AS1 has been linked to the pathogenesis of a wide range of tumors. Nevertheless, whether it plays a role in laryngeal carcinoma (LC) remains unclear. METHODS: Twenty-nine pairs of LC and related normal tissues were collected for the detection of lncRNA AFAP1-AS1 using qRT-PC...

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Autores principales: Chen, Xin, Hu, Ziwei, Bing, Liao, Zhu, Xinhua, Liu, Ke, Liu, Yuehui, Liu, Jianguo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9371880/
https://www.ncbi.nlm.nih.gov/pubmed/35968243
http://dx.doi.org/10.1155/2022/2337447
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author Chen, Xin
Hu, Ziwei
Bing, Liao
Zhu, Xinhua
Liu, Ke
Liu, Yuehui
Liu, Jianguo
author_facet Chen, Xin
Hu, Ziwei
Bing, Liao
Zhu, Xinhua
Liu, Ke
Liu, Yuehui
Liu, Jianguo
author_sort Chen, Xin
collection PubMed
description BACKGROUND: lncRNA AFAP1-AS1 has been linked to the pathogenesis of a wide range of tumors. Nevertheless, whether it plays a role in laryngeal carcinoma (LC) remains unclear. METHODS: Twenty-nine pairs of LC and related normal tissues were collected for the detection of lncRNA AFAP1-AS1 using qRT-PCR. Correlation of lncRNA AFAP1-AS1 level and clinicopathological characters was assessed by the chi-square test. Impacts of lncRNA AFAP1-AS1 silencing on LC phenotypes were tested in vitro via CCK-8, clone formation, EdU staining, wound healing, flow cytometry, and Transwell assay. RESULTS: Herein, a remarkable elevation of lncRNA AFAP1-AS1 was observed in LC patients. And higher lncRNA AFAP1-AS1 level was correlated to worse clinical pathological characteristics. Moreover, lncRNA AFAP1-AS1 silencing was revealed to repress TU212 malignant phenotypes. CONCLUSION: Our data suggested that lncRNA AFAP1-AS1 acts as an oncogene of LC in vitro.
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spelling pubmed-93718802022-08-12 The Expression of the Long Noncoding RNA AFAP1-AS1 in Laryngeal Carcinoma Affects the Proliferation, Invasion, Migration, and Apoptosis of TU212 Cell Line Chen, Xin Hu, Ziwei Bing, Liao Zhu, Xinhua Liu, Ke Liu, Yuehui Liu, Jianguo Biomed Res Int Research Article BACKGROUND: lncRNA AFAP1-AS1 has been linked to the pathogenesis of a wide range of tumors. Nevertheless, whether it plays a role in laryngeal carcinoma (LC) remains unclear. METHODS: Twenty-nine pairs of LC and related normal tissues were collected for the detection of lncRNA AFAP1-AS1 using qRT-PCR. Correlation of lncRNA AFAP1-AS1 level and clinicopathological characters was assessed by the chi-square test. Impacts of lncRNA AFAP1-AS1 silencing on LC phenotypes were tested in vitro via CCK-8, clone formation, EdU staining, wound healing, flow cytometry, and Transwell assay. RESULTS: Herein, a remarkable elevation of lncRNA AFAP1-AS1 was observed in LC patients. And higher lncRNA AFAP1-AS1 level was correlated to worse clinical pathological characteristics. Moreover, lncRNA AFAP1-AS1 silencing was revealed to repress TU212 malignant phenotypes. CONCLUSION: Our data suggested that lncRNA AFAP1-AS1 acts as an oncogene of LC in vitro. Hindawi 2022-08-04 /pmc/articles/PMC9371880/ /pubmed/35968243 http://dx.doi.org/10.1155/2022/2337447 Text en Copyright © 2022 Xin Chen et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Chen, Xin
Hu, Ziwei
Bing, Liao
Zhu, Xinhua
Liu, Ke
Liu, Yuehui
Liu, Jianguo
The Expression of the Long Noncoding RNA AFAP1-AS1 in Laryngeal Carcinoma Affects the Proliferation, Invasion, Migration, and Apoptosis of TU212 Cell Line
title The Expression of the Long Noncoding RNA AFAP1-AS1 in Laryngeal Carcinoma Affects the Proliferation, Invasion, Migration, and Apoptosis of TU212 Cell Line
title_full The Expression of the Long Noncoding RNA AFAP1-AS1 in Laryngeal Carcinoma Affects the Proliferation, Invasion, Migration, and Apoptosis of TU212 Cell Line
title_fullStr The Expression of the Long Noncoding RNA AFAP1-AS1 in Laryngeal Carcinoma Affects the Proliferation, Invasion, Migration, and Apoptosis of TU212 Cell Line
title_full_unstemmed The Expression of the Long Noncoding RNA AFAP1-AS1 in Laryngeal Carcinoma Affects the Proliferation, Invasion, Migration, and Apoptosis of TU212 Cell Line
title_short The Expression of the Long Noncoding RNA AFAP1-AS1 in Laryngeal Carcinoma Affects the Proliferation, Invasion, Migration, and Apoptosis of TU212 Cell Line
title_sort expression of the long noncoding rna afap1-as1 in laryngeal carcinoma affects the proliferation, invasion, migration, and apoptosis of tu212 cell line
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9371880/
https://www.ncbi.nlm.nih.gov/pubmed/35968243
http://dx.doi.org/10.1155/2022/2337447
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