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Quantitative, multiplexed, targeted proteomics for ascertaining variant specific SARS-CoV-2 antibody response

Determining the protection an individual has to severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) variants of concern (VoCs) is crucial for future immune surveillance, vaccine development, and understanding of the changing immune response. We devised an informative assay to current ELISA-...

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Autores principales: Doykov, Ivan, Baldwin, Tomas, Spiewak, Justyna, Gilmour, Kimberly C., Gibbons, Joseph M., Pade, Corinna, Reynolds, Catherine J., Áine McKnight, Noursadeghi, Mahdad, Maini, Mala K., Manisty, Charlotte, Treibel, Thomas, Captur, Gabriella, Fontana, Marianna, Boyton, Rosemary J., Altmann, Daniel M., Brooks, Tim, Semper, Amanda, Moon, James C., Kevin Mills, Heywood, Wendy E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9372021/
https://www.ncbi.nlm.nih.gov/pubmed/35975199
http://dx.doi.org/10.1016/j.crmeth.2022.100279
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author Doykov, Ivan
Baldwin, Tomas
Spiewak, Justyna
Gilmour, Kimberly C.
Gibbons, Joseph M.
Pade, Corinna
Reynolds, Catherine J.
Áine McKnight
Noursadeghi, Mahdad
Maini, Mala K.
Manisty, Charlotte
Treibel, Thomas
Captur, Gabriella
Fontana, Marianna
Boyton, Rosemary J.
Altmann, Daniel M.
Brooks, Tim
Semper, Amanda
Moon, James C.
Kevin Mills
Heywood, Wendy E.
author_facet Doykov, Ivan
Baldwin, Tomas
Spiewak, Justyna
Gilmour, Kimberly C.
Gibbons, Joseph M.
Pade, Corinna
Reynolds, Catherine J.
Áine McKnight
Noursadeghi, Mahdad
Maini, Mala K.
Manisty, Charlotte
Treibel, Thomas
Captur, Gabriella
Fontana, Marianna
Boyton, Rosemary J.
Altmann, Daniel M.
Brooks, Tim
Semper, Amanda
Moon, James C.
Kevin Mills
Heywood, Wendy E.
author_sort Doykov, Ivan
collection PubMed
description Determining the protection an individual has to severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) variants of concern (VoCs) is crucial for future immune surveillance, vaccine development, and understanding of the changing immune response. We devised an informative assay to current ELISA-based serology using multiplexed, baited, targeted proteomics for direct detection of multiple proteins in the SARS-CoV-2 anti-spike antibody immunocomplex. Serum from individuals collected after infection or first- and second-dose vaccination demonstrates this approach and shows concordance with existing serology and neutralization. Our assays show altered responses of both immunoglobulins and complement to the Alpha (B.1.1.7), Beta (B.1.351), and Delta (B.1.617.1) VoCs and a reduced response to Omicron (B1.1.1529). We were able to identify individuals who had prior infection, and observed that C1q is closely associated with IgG1 (r > 0.82) and may better reflect neutralization to VoCs. Analyzing additional immunoproteins beyond immunoglobulin (Ig) G, provides important information about our understanding of the response to infection and vaccination.
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spelling pubmed-93720212022-08-12 Quantitative, multiplexed, targeted proteomics for ascertaining variant specific SARS-CoV-2 antibody response Doykov, Ivan Baldwin, Tomas Spiewak, Justyna Gilmour, Kimberly C. Gibbons, Joseph M. Pade, Corinna Reynolds, Catherine J. Áine McKnight Noursadeghi, Mahdad Maini, Mala K. Manisty, Charlotte Treibel, Thomas Captur, Gabriella Fontana, Marianna Boyton, Rosemary J. Altmann, Daniel M. Brooks, Tim Semper, Amanda Moon, James C. Kevin Mills Heywood, Wendy E. Cell Rep Methods Article Determining the protection an individual has to severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) variants of concern (VoCs) is crucial for future immune surveillance, vaccine development, and understanding of the changing immune response. We devised an informative assay to current ELISA-based serology using multiplexed, baited, targeted proteomics for direct detection of multiple proteins in the SARS-CoV-2 anti-spike antibody immunocomplex. Serum from individuals collected after infection or first- and second-dose vaccination demonstrates this approach and shows concordance with existing serology and neutralization. Our assays show altered responses of both immunoglobulins and complement to the Alpha (B.1.1.7), Beta (B.1.351), and Delta (B.1.617.1) VoCs and a reduced response to Omicron (B1.1.1529). We were able to identify individuals who had prior infection, and observed that C1q is closely associated with IgG1 (r > 0.82) and may better reflect neutralization to VoCs. Analyzing additional immunoproteins beyond immunoglobulin (Ig) G, provides important information about our understanding of the response to infection and vaccination. Elsevier 2022-08-12 /pmc/articles/PMC9372021/ /pubmed/35975199 http://dx.doi.org/10.1016/j.crmeth.2022.100279 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Doykov, Ivan
Baldwin, Tomas
Spiewak, Justyna
Gilmour, Kimberly C.
Gibbons, Joseph M.
Pade, Corinna
Reynolds, Catherine J.
Áine McKnight
Noursadeghi, Mahdad
Maini, Mala K.
Manisty, Charlotte
Treibel, Thomas
Captur, Gabriella
Fontana, Marianna
Boyton, Rosemary J.
Altmann, Daniel M.
Brooks, Tim
Semper, Amanda
Moon, James C.
Kevin Mills
Heywood, Wendy E.
Quantitative, multiplexed, targeted proteomics for ascertaining variant specific SARS-CoV-2 antibody response
title Quantitative, multiplexed, targeted proteomics for ascertaining variant specific SARS-CoV-2 antibody response
title_full Quantitative, multiplexed, targeted proteomics for ascertaining variant specific SARS-CoV-2 antibody response
title_fullStr Quantitative, multiplexed, targeted proteomics for ascertaining variant specific SARS-CoV-2 antibody response
title_full_unstemmed Quantitative, multiplexed, targeted proteomics for ascertaining variant specific SARS-CoV-2 antibody response
title_short Quantitative, multiplexed, targeted proteomics for ascertaining variant specific SARS-CoV-2 antibody response
title_sort quantitative, multiplexed, targeted proteomics for ascertaining variant specific sars-cov-2 antibody response
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9372021/
https://www.ncbi.nlm.nih.gov/pubmed/35975199
http://dx.doi.org/10.1016/j.crmeth.2022.100279
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