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Isolation and Identification of Ice Nucleation Active Fusarium Strains from Rapid Apple Declined Trees in Korea

In biological particles such as Fusarium species, ice nucleation activity (INA) has been observed. Fusarium strains isolated from apple declined trees in Korea were identified with a multilocus sequence analysis using the tef1 and rpb1 genes. Droplet-freezing and tube-freezing assays were used to de...

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Autores principales: Avalos-Ruiz, Diane, Ten, Leonid N., Kim, Chang-Kil, Lee, Seung-Yeol, Jung, Hee-Young
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society of Plant Pathology 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9372094/
https://www.ncbi.nlm.nih.gov/pubmed/35953060
http://dx.doi.org/10.5423/PPJ.NT.04.2022.0051
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author Avalos-Ruiz, Diane
Ten, Leonid N.
Kim, Chang-Kil
Lee, Seung-Yeol
Jung, Hee-Young
author_facet Avalos-Ruiz, Diane
Ten, Leonid N.
Kim, Chang-Kil
Lee, Seung-Yeol
Jung, Hee-Young
author_sort Avalos-Ruiz, Diane
collection PubMed
description In biological particles such as Fusarium species, ice nucleation activity (INA) has been observed. Fusarium strains isolated from apple declined trees in Korea were identified with a multilocus sequence analysis using the tef1 and rpb1 genes. Droplet-freezing and tube-freezing assays were used to determine the INA of the strains, using Pseudomonas syringae pv. syringae KACC 21200 as a positive control and resulting in seven INA+ fungal strains that were identified as F. tricinctum (KNUF-21-F17, KNUF-21-F18, KNUF-21-F29, KNUF-21-F32, KNUF-21-F38, KNUF-21-F43, and KNUF-21-F44). The effect of Fusarium INA+ KNUF-21-F29 was compared to that of INA– strains on Chrysanthemum morifolium cv. Shinma explants. A higher callus formation and no-shoot formation were observed, suggesting that fungal INA could play a role in cold injuries and be a factor to consider in rapid apple decline. To the best of our knowledge, this is the first report of INA fungal strains isolated in Korea.
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spelling pubmed-93720942022-08-19 Isolation and Identification of Ice Nucleation Active Fusarium Strains from Rapid Apple Declined Trees in Korea Avalos-Ruiz, Diane Ten, Leonid N. Kim, Chang-Kil Lee, Seung-Yeol Jung, Hee-Young Plant Pathol J Note In biological particles such as Fusarium species, ice nucleation activity (INA) has been observed. Fusarium strains isolated from apple declined trees in Korea were identified with a multilocus sequence analysis using the tef1 and rpb1 genes. Droplet-freezing and tube-freezing assays were used to determine the INA of the strains, using Pseudomonas syringae pv. syringae KACC 21200 as a positive control and resulting in seven INA+ fungal strains that were identified as F. tricinctum (KNUF-21-F17, KNUF-21-F18, KNUF-21-F29, KNUF-21-F32, KNUF-21-F38, KNUF-21-F43, and KNUF-21-F44). The effect of Fusarium INA+ KNUF-21-F29 was compared to that of INA– strains on Chrysanthemum morifolium cv. Shinma explants. A higher callus formation and no-shoot formation were observed, suggesting that fungal INA could play a role in cold injuries and be a factor to consider in rapid apple decline. To the best of our knowledge, this is the first report of INA fungal strains isolated in Korea. Korean Society of Plant Pathology 2022-08 2022-08-01 /pmc/articles/PMC9372094/ /pubmed/35953060 http://dx.doi.org/10.5423/PPJ.NT.04.2022.0051 Text en © The Korean Society of Plant Pathology https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Note
Avalos-Ruiz, Diane
Ten, Leonid N.
Kim, Chang-Kil
Lee, Seung-Yeol
Jung, Hee-Young
Isolation and Identification of Ice Nucleation Active Fusarium Strains from Rapid Apple Declined Trees in Korea
title Isolation and Identification of Ice Nucleation Active Fusarium Strains from Rapid Apple Declined Trees in Korea
title_full Isolation and Identification of Ice Nucleation Active Fusarium Strains from Rapid Apple Declined Trees in Korea
title_fullStr Isolation and Identification of Ice Nucleation Active Fusarium Strains from Rapid Apple Declined Trees in Korea
title_full_unstemmed Isolation and Identification of Ice Nucleation Active Fusarium Strains from Rapid Apple Declined Trees in Korea
title_short Isolation and Identification of Ice Nucleation Active Fusarium Strains from Rapid Apple Declined Trees in Korea
title_sort isolation and identification of ice nucleation active fusarium strains from rapid apple declined trees in korea
topic Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9372094/
https://www.ncbi.nlm.nih.gov/pubmed/35953060
http://dx.doi.org/10.5423/PPJ.NT.04.2022.0051
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