Cargando…
Detection and Quantification of Apple Stem Grooving Virus in Micropropagated Apple Plantlets Using Reverse-Transcription Droplet Digital PCR
Apple stem grooving virus (ASGV) is a destructive viral pathogen of pome fruit trees that causes significant losses to fruit production worldwide. Obtaining ASGV-free propagation materials is essential to reduce economic losses, and accurate and sensitive detection methods to screen ASGV-free plantl...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Korean Society of Plant Pathology
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9372104/ https://www.ncbi.nlm.nih.gov/pubmed/35953062 http://dx.doi.org/10.5423/PPJ.NT.05.2022.0072 |
_version_ | 1784767307563663360 |
---|---|
author | Kim, Sung-Woong Lee, Hyo-Jeong Cho, Kang Hee Jeong, Rae-Dong |
author_facet | Kim, Sung-Woong Lee, Hyo-Jeong Cho, Kang Hee Jeong, Rae-Dong |
author_sort | Kim, Sung-Woong |
collection | PubMed |
description | Apple stem grooving virus (ASGV) is a destructive viral pathogen of pome fruit trees that causes significant losses to fruit production worldwide. Obtaining ASGV-free propagation materials is essential to reduce economic losses, and accurate and sensitive detection methods to screen ASGV-free plantlets during in vitro propagation are urgently necessary. In this study, ASGV was sensitively and accurately quantified from in vitro propagated apple plantlets using a reverse transcription droplet digital polymerase chain reaction (RT-ddPCR) assay. The optimized RT-ddPCR assay was specific to other apple viruses, and was at least 10-times more sensitive than RT-real-time quantitative PCR assay. Furthermore, the optimized RT-ddPCR assay was validated for the detection and quantification of ASGV using micropropagated apple plantlet samples. This RT-ddPCR assay can be utilized for the accurate quantitative detection of ASGV infection in ASGV-free certification programs, and can thus contribute to the production of ASGV-free apple trees. |
format | Online Article Text |
id | pubmed-9372104 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Korean Society of Plant Pathology |
record_format | MEDLINE/PubMed |
spelling | pubmed-93721042022-08-19 Detection and Quantification of Apple Stem Grooving Virus in Micropropagated Apple Plantlets Using Reverse-Transcription Droplet Digital PCR Kim, Sung-Woong Lee, Hyo-Jeong Cho, Kang Hee Jeong, Rae-Dong Plant Pathol J Note Apple stem grooving virus (ASGV) is a destructive viral pathogen of pome fruit trees that causes significant losses to fruit production worldwide. Obtaining ASGV-free propagation materials is essential to reduce economic losses, and accurate and sensitive detection methods to screen ASGV-free plantlets during in vitro propagation are urgently necessary. In this study, ASGV was sensitively and accurately quantified from in vitro propagated apple plantlets using a reverse transcription droplet digital polymerase chain reaction (RT-ddPCR) assay. The optimized RT-ddPCR assay was specific to other apple viruses, and was at least 10-times more sensitive than RT-real-time quantitative PCR assay. Furthermore, the optimized RT-ddPCR assay was validated for the detection and quantification of ASGV using micropropagated apple plantlet samples. This RT-ddPCR assay can be utilized for the accurate quantitative detection of ASGV infection in ASGV-free certification programs, and can thus contribute to the production of ASGV-free apple trees. Korean Society of Plant Pathology 2022-08 2022-08-01 /pmc/articles/PMC9372104/ /pubmed/35953062 http://dx.doi.org/10.5423/PPJ.NT.05.2022.0072 Text en © The Korean Society of Plant Pathology https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Note Kim, Sung-Woong Lee, Hyo-Jeong Cho, Kang Hee Jeong, Rae-Dong Detection and Quantification of Apple Stem Grooving Virus in Micropropagated Apple Plantlets Using Reverse-Transcription Droplet Digital PCR |
title | Detection and Quantification of Apple Stem Grooving Virus in Micropropagated Apple Plantlets Using Reverse-Transcription Droplet Digital PCR |
title_full | Detection and Quantification of Apple Stem Grooving Virus in Micropropagated Apple Plantlets Using Reverse-Transcription Droplet Digital PCR |
title_fullStr | Detection and Quantification of Apple Stem Grooving Virus in Micropropagated Apple Plantlets Using Reverse-Transcription Droplet Digital PCR |
title_full_unstemmed | Detection and Quantification of Apple Stem Grooving Virus in Micropropagated Apple Plantlets Using Reverse-Transcription Droplet Digital PCR |
title_short | Detection and Quantification of Apple Stem Grooving Virus in Micropropagated Apple Plantlets Using Reverse-Transcription Droplet Digital PCR |
title_sort | detection and quantification of apple stem grooving virus in micropropagated apple plantlets using reverse-transcription droplet digital pcr |
topic | Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9372104/ https://www.ncbi.nlm.nih.gov/pubmed/35953062 http://dx.doi.org/10.5423/PPJ.NT.05.2022.0072 |
work_keys_str_mv | AT kimsungwoong detectionandquantificationofapplestemgroovingvirusinmicropropagatedappleplantletsusingreversetranscriptiondropletdigitalpcr AT leehyojeong detectionandquantificationofapplestemgroovingvirusinmicropropagatedappleplantletsusingreversetranscriptiondropletdigitalpcr AT chokanghee detectionandquantificationofapplestemgroovingvirusinmicropropagatedappleplantletsusingreversetranscriptiondropletdigitalpcr AT jeongraedong detectionandquantificationofapplestemgroovingvirusinmicropropagatedappleplantletsusingreversetranscriptiondropletdigitalpcr |