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Harnessing originally robust yeast for rapid lactic acid bioproduction without detoxification and neutralization
Acidic and chemical inhibitor stresses undermine efficient lactic acid bioproduction from lignocellulosic feedstock. Requisite coping treatments, such as detoxification and neutralizing agent supplementation, can be eliminated if a strong microbial host is employed in the process. Here, we exploited...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9372150/ https://www.ncbi.nlm.nih.gov/pubmed/35953496 http://dx.doi.org/10.1038/s41598-022-17737-4 |
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author | Pangestu, Radityo Kahar, Prihardi Kholida, Lutfi Nia Perwitasari, Urip Thontowi, Ahmad Fahrurrozi Lisdiyanti, Puspita Yopi Ogino, Chiaki Prasetya, Bambang Kondo, Akihiko |
author_facet | Pangestu, Radityo Kahar, Prihardi Kholida, Lutfi Nia Perwitasari, Urip Thontowi, Ahmad Fahrurrozi Lisdiyanti, Puspita Yopi Ogino, Chiaki Prasetya, Bambang Kondo, Akihiko |
author_sort | Pangestu, Radityo |
collection | PubMed |
description | Acidic and chemical inhibitor stresses undermine efficient lactic acid bioproduction from lignocellulosic feedstock. Requisite coping treatments, such as detoxification and neutralizing agent supplementation, can be eliminated if a strong microbial host is employed in the process. Here, we exploited an originally robust yeast, Saccharomyces cerevisiae BTCC3, as a production platform for lactic acid. This wild-type strain exhibited a rapid cell growth in the presence of various chemical inhibitors compared to laboratory and industrial strains, namely BY4741 and Ethanol-red. Pathway engineering was performed on the strain by introducing an exogenous LDH gene after disrupting the PDC1 and PDC5 genes. Facilitated by this engineered strain, high cell density cultivation could generate lactic acid with productivity at 4.80 and 3.68 g L(−1) h(−1) under semi-neutralized and non-neutralized conditions, respectively. Those values were relatively higher compared to other studies. Cultivation using real lignocellulosic hydrolysate was conducted to assess the performance of this engineered strain. Non-neutralized fermentation using non-detoxified hydrolysate from sugarcane bagasse as a medium could produce lactic acid at 1.69 g L(−1) h(−1), which was competitive to the results from other reports that still included detoxification and neutralization steps in their experiments. This strategy could make the overall lactic acid bioproduction process simpler, greener, and more cost-efficient. |
format | Online Article Text |
id | pubmed-9372150 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-93721502022-08-13 Harnessing originally robust yeast for rapid lactic acid bioproduction without detoxification and neutralization Pangestu, Radityo Kahar, Prihardi Kholida, Lutfi Nia Perwitasari, Urip Thontowi, Ahmad Fahrurrozi Lisdiyanti, Puspita Yopi Ogino, Chiaki Prasetya, Bambang Kondo, Akihiko Sci Rep Article Acidic and chemical inhibitor stresses undermine efficient lactic acid bioproduction from lignocellulosic feedstock. Requisite coping treatments, such as detoxification and neutralizing agent supplementation, can be eliminated if a strong microbial host is employed in the process. Here, we exploited an originally robust yeast, Saccharomyces cerevisiae BTCC3, as a production platform for lactic acid. This wild-type strain exhibited a rapid cell growth in the presence of various chemical inhibitors compared to laboratory and industrial strains, namely BY4741 and Ethanol-red. Pathway engineering was performed on the strain by introducing an exogenous LDH gene after disrupting the PDC1 and PDC5 genes. Facilitated by this engineered strain, high cell density cultivation could generate lactic acid with productivity at 4.80 and 3.68 g L(−1) h(−1) under semi-neutralized and non-neutralized conditions, respectively. Those values were relatively higher compared to other studies. Cultivation using real lignocellulosic hydrolysate was conducted to assess the performance of this engineered strain. Non-neutralized fermentation using non-detoxified hydrolysate from sugarcane bagasse as a medium could produce lactic acid at 1.69 g L(−1) h(−1), which was competitive to the results from other reports that still included detoxification and neutralization steps in their experiments. This strategy could make the overall lactic acid bioproduction process simpler, greener, and more cost-efficient. Nature Publishing Group UK 2022-08-11 /pmc/articles/PMC9372150/ /pubmed/35953496 http://dx.doi.org/10.1038/s41598-022-17737-4 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Pangestu, Radityo Kahar, Prihardi Kholida, Lutfi Nia Perwitasari, Urip Thontowi, Ahmad Fahrurrozi Lisdiyanti, Puspita Yopi Ogino, Chiaki Prasetya, Bambang Kondo, Akihiko Harnessing originally robust yeast for rapid lactic acid bioproduction without detoxification and neutralization |
title | Harnessing originally robust yeast for rapid lactic acid bioproduction without detoxification and neutralization |
title_full | Harnessing originally robust yeast for rapid lactic acid bioproduction without detoxification and neutralization |
title_fullStr | Harnessing originally robust yeast for rapid lactic acid bioproduction without detoxification and neutralization |
title_full_unstemmed | Harnessing originally robust yeast for rapid lactic acid bioproduction without detoxification and neutralization |
title_short | Harnessing originally robust yeast for rapid lactic acid bioproduction without detoxification and neutralization |
title_sort | harnessing originally robust yeast for rapid lactic acid bioproduction without detoxification and neutralization |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9372150/ https://www.ncbi.nlm.nih.gov/pubmed/35953496 http://dx.doi.org/10.1038/s41598-022-17737-4 |
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