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Antibiofilm property and multiple action of peptide PEW300 against Pseudomonas aeruginosa

Pseudomonas aeruginosa (P. aeruginosa), an opportunistic pathogen, is often associated with difficulties in treating hospital-acquired infections. Biofilms formed by P. aeruginosa significantly improve its resistance to antimicrobial agents, thereby, posing a great challenge to the combat of P. aeru...

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Autores principales: Wang, Meng, Deng, Zifeng, Li, Yanmei, Xu, Keyong, Ma, Yi, Yang, Shang-Tian, Wang, Jufang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9372277/
https://www.ncbi.nlm.nih.gov/pubmed/35966656
http://dx.doi.org/10.3389/fmicb.2022.963292
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author Wang, Meng
Deng, Zifeng
Li, Yanmei
Xu, Keyong
Ma, Yi
Yang, Shang-Tian
Wang, Jufang
author_facet Wang, Meng
Deng, Zifeng
Li, Yanmei
Xu, Keyong
Ma, Yi
Yang, Shang-Tian
Wang, Jufang
author_sort Wang, Meng
collection PubMed
description Pseudomonas aeruginosa (P. aeruginosa), an opportunistic pathogen, is often associated with difficulties in treating hospital-acquired infections. Biofilms formed by P. aeruginosa significantly improve its resistance to antimicrobial agents, thereby, posing a great challenge to the combat of P. aeruginosa infection. Antimicrobial peptides (AMPs) have recently emerged as promising antibiofilm agents and increasingly attracting the attention of scientists worldwide. However, current knowledge of their antibiofilm behavior is limited and their underlying mechanism remains unclear. In this study, a novel AMP, named PEW300, with three-point mutations (E9H, D17K, and T33A) from Cecropin A was used to investigate its antibiofilm property and antibiofilm pathway against P. aeruginosa. PEW300 displayed strong antibacterial and antibiofilm activity against P. aeruginosa with no significant hemolysis or cytotoxicity to mouse erythrocyte and human embryonic kidney 293 cells. Besides, the antibiofilm pathway results showed that PEW300 preferentially dispersed the mature biofilm, leading to the biofilm-encapsulated bacteria exposure and death. Meanwhile, we also found that the extracellular DNA was a critical target of PEW300 against the mature biofilm of P. aeruginosa. In addition, multiple actions of PEW300 including destroying the cell membrane integrity, inducing high levels of intracellular reactive oxygen species, and interacting with genomic DNA were adopted to exert its antibacterial activity. Moreover, PEW300 could dramatically reduce the virulence of P. aeruginosa. Taken together, PEW300 might be served as a promising antibiofilm candidate to combat P. aeruginosa biofilms.
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spelling pubmed-93722772022-08-13 Antibiofilm property and multiple action of peptide PEW300 against Pseudomonas aeruginosa Wang, Meng Deng, Zifeng Li, Yanmei Xu, Keyong Ma, Yi Yang, Shang-Tian Wang, Jufang Front Microbiol Microbiology Pseudomonas aeruginosa (P. aeruginosa), an opportunistic pathogen, is often associated with difficulties in treating hospital-acquired infections. Biofilms formed by P. aeruginosa significantly improve its resistance to antimicrobial agents, thereby, posing a great challenge to the combat of P. aeruginosa infection. Antimicrobial peptides (AMPs) have recently emerged as promising antibiofilm agents and increasingly attracting the attention of scientists worldwide. However, current knowledge of their antibiofilm behavior is limited and their underlying mechanism remains unclear. In this study, a novel AMP, named PEW300, with three-point mutations (E9H, D17K, and T33A) from Cecropin A was used to investigate its antibiofilm property and antibiofilm pathway against P. aeruginosa. PEW300 displayed strong antibacterial and antibiofilm activity against P. aeruginosa with no significant hemolysis or cytotoxicity to mouse erythrocyte and human embryonic kidney 293 cells. Besides, the antibiofilm pathway results showed that PEW300 preferentially dispersed the mature biofilm, leading to the biofilm-encapsulated bacteria exposure and death. Meanwhile, we also found that the extracellular DNA was a critical target of PEW300 against the mature biofilm of P. aeruginosa. In addition, multiple actions of PEW300 including destroying the cell membrane integrity, inducing high levels of intracellular reactive oxygen species, and interacting with genomic DNA were adopted to exert its antibacterial activity. Moreover, PEW300 could dramatically reduce the virulence of P. aeruginosa. Taken together, PEW300 might be served as a promising antibiofilm candidate to combat P. aeruginosa biofilms. Frontiers Media S.A. 2022-07-29 /pmc/articles/PMC9372277/ /pubmed/35966656 http://dx.doi.org/10.3389/fmicb.2022.963292 Text en Copyright © 2022 Wang, Deng, Li, Xu, Ma, Yang and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Wang, Meng
Deng, Zifeng
Li, Yanmei
Xu, Keyong
Ma, Yi
Yang, Shang-Tian
Wang, Jufang
Antibiofilm property and multiple action of peptide PEW300 against Pseudomonas aeruginosa
title Antibiofilm property and multiple action of peptide PEW300 against Pseudomonas aeruginosa
title_full Antibiofilm property and multiple action of peptide PEW300 against Pseudomonas aeruginosa
title_fullStr Antibiofilm property and multiple action of peptide PEW300 against Pseudomonas aeruginosa
title_full_unstemmed Antibiofilm property and multiple action of peptide PEW300 against Pseudomonas aeruginosa
title_short Antibiofilm property and multiple action of peptide PEW300 against Pseudomonas aeruginosa
title_sort antibiofilm property and multiple action of peptide pew300 against pseudomonas aeruginosa
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9372277/
https://www.ncbi.nlm.nih.gov/pubmed/35966656
http://dx.doi.org/10.3389/fmicb.2022.963292
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