Cargando…

Constitutive expression of VviNAC17 transcription factor significantly induces the synthesis of flavonoids and other phenolics in transgenic grape berry cells

VviNAC17 is a grapevine transcription factor activated by ABA. Because ABA has been proposed as the main signal modulating the secondary metabolism in grape berry skins, here we postulated VviNAC17 as a positive regulator of secondary metabolism in grape cells. To validate the hypothesis, VviNAC17 w...

Descripción completa

Detalles Bibliográficos
Autores principales: Badim, Hélder, Vale, Mariana, Coelho, Marco, Granell, Antonio, Gerós, Hernâni, Conde, Artur
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9372392/
https://www.ncbi.nlm.nih.gov/pubmed/35968093
http://dx.doi.org/10.3389/fpls.2022.964621
_version_ 1784767371432427520
author Badim, Hélder
Vale, Mariana
Coelho, Marco
Granell, Antonio
Gerós, Hernâni
Conde, Artur
author_facet Badim, Hélder
Vale, Mariana
Coelho, Marco
Granell, Antonio
Gerós, Hernâni
Conde, Artur
author_sort Badim, Hélder
collection PubMed
description VviNAC17 is a grapevine transcription factor activated by ABA. Because ABA has been proposed as the main signal modulating the secondary metabolism in grape berry skins, here we postulated VviNAC17 as a positive regulator of secondary metabolism in grape cells. To validate the hypothesis, VviNAC17 was constitutively and stably overexpressed in grape berry suspension-cultured cells of Gamay Fréaux cv. by Agrobacterium-mediated transformation. Targeted transcriptional analyses by qPCR showed that several genes involved the phenylpropanoid (VviPAL1), stilbenoid (VviSTS1) and flavonoid pathways (VviDFR, VviLAR1, VviANR, VviLDOX, and VviUFGT1), as well as anthocyanin vacuolar transport and accumulation (VviGST4 and VvMATE1) were significantly upregulated in VviNAC17-overexpressing transgenic cells, which translated in the stimulation of a number of enzymatic activities in those pathways. This was the case of phenylalanine ammonia lyase (PAL) and UDP-glucose:flavonoid 3-O-glucosyltransferase (UFGT) that were about 2-fold and 3.5-fold higher in VviNAC17-overexpressing cells than in control cells. VviNAC17-overexpressing cells accumulated significantly higher amounts of anthocyanins, proanthocyanidins, total flavonoids and total phenolics. These findings confirmed that VviNAC17 is an important positive regulator of secondary metabolism in grapevine contributing to the accumulation of important berry quality-related secondary metabolites.
format Online
Article
Text
id pubmed-9372392
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-93723922022-08-13 Constitutive expression of VviNAC17 transcription factor significantly induces the synthesis of flavonoids and other phenolics in transgenic grape berry cells Badim, Hélder Vale, Mariana Coelho, Marco Granell, Antonio Gerós, Hernâni Conde, Artur Front Plant Sci Plant Science VviNAC17 is a grapevine transcription factor activated by ABA. Because ABA has been proposed as the main signal modulating the secondary metabolism in grape berry skins, here we postulated VviNAC17 as a positive regulator of secondary metabolism in grape cells. To validate the hypothesis, VviNAC17 was constitutively and stably overexpressed in grape berry suspension-cultured cells of Gamay Fréaux cv. by Agrobacterium-mediated transformation. Targeted transcriptional analyses by qPCR showed that several genes involved the phenylpropanoid (VviPAL1), stilbenoid (VviSTS1) and flavonoid pathways (VviDFR, VviLAR1, VviANR, VviLDOX, and VviUFGT1), as well as anthocyanin vacuolar transport and accumulation (VviGST4 and VvMATE1) were significantly upregulated in VviNAC17-overexpressing transgenic cells, which translated in the stimulation of a number of enzymatic activities in those pathways. This was the case of phenylalanine ammonia lyase (PAL) and UDP-glucose:flavonoid 3-O-glucosyltransferase (UFGT) that were about 2-fold and 3.5-fold higher in VviNAC17-overexpressing cells than in control cells. VviNAC17-overexpressing cells accumulated significantly higher amounts of anthocyanins, proanthocyanidins, total flavonoids and total phenolics. These findings confirmed that VviNAC17 is an important positive regulator of secondary metabolism in grapevine contributing to the accumulation of important berry quality-related secondary metabolites. Frontiers Media S.A. 2022-07-29 /pmc/articles/PMC9372392/ /pubmed/35968093 http://dx.doi.org/10.3389/fpls.2022.964621 Text en Copyright © 2022 Badim, Vale, Coelho, Granell, Gerós and Conde. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Badim, Hélder
Vale, Mariana
Coelho, Marco
Granell, Antonio
Gerós, Hernâni
Conde, Artur
Constitutive expression of VviNAC17 transcription factor significantly induces the synthesis of flavonoids and other phenolics in transgenic grape berry cells
title Constitutive expression of VviNAC17 transcription factor significantly induces the synthesis of flavonoids and other phenolics in transgenic grape berry cells
title_full Constitutive expression of VviNAC17 transcription factor significantly induces the synthesis of flavonoids and other phenolics in transgenic grape berry cells
title_fullStr Constitutive expression of VviNAC17 transcription factor significantly induces the synthesis of flavonoids and other phenolics in transgenic grape berry cells
title_full_unstemmed Constitutive expression of VviNAC17 transcription factor significantly induces the synthesis of flavonoids and other phenolics in transgenic grape berry cells
title_short Constitutive expression of VviNAC17 transcription factor significantly induces the synthesis of flavonoids and other phenolics in transgenic grape berry cells
title_sort constitutive expression of vvinac17 transcription factor significantly induces the synthesis of flavonoids and other phenolics in transgenic grape berry cells
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9372392/
https://www.ncbi.nlm.nih.gov/pubmed/35968093
http://dx.doi.org/10.3389/fpls.2022.964621
work_keys_str_mv AT badimhelder constitutiveexpressionofvvinac17transcriptionfactorsignificantlyinducesthesynthesisofflavonoidsandotherphenolicsintransgenicgrapeberrycells
AT valemariana constitutiveexpressionofvvinac17transcriptionfactorsignificantlyinducesthesynthesisofflavonoidsandotherphenolicsintransgenicgrapeberrycells
AT coelhomarco constitutiveexpressionofvvinac17transcriptionfactorsignificantlyinducesthesynthesisofflavonoidsandotherphenolicsintransgenicgrapeberrycells
AT granellantonio constitutiveexpressionofvvinac17transcriptionfactorsignificantlyinducesthesynthesisofflavonoidsandotherphenolicsintransgenicgrapeberrycells
AT geroshernani constitutiveexpressionofvvinac17transcriptionfactorsignificantlyinducesthesynthesisofflavonoidsandotherphenolicsintransgenicgrapeberrycells
AT condeartur constitutiveexpressionofvvinac17transcriptionfactorsignificantlyinducesthesynthesisofflavonoidsandotherphenolicsintransgenicgrapeberrycells