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Different serotypes of Escherichia coli flagellin exert identical adjuvant effects

Bacterial flagellin is a potent powerful adjuvant, which exerts its adjuvant activity by activating the Toll-like receptor 5 (TLR5) signaling pathway to induce host pro-inflammatory responses. Flagellin of Salmonella typhimurium (S. typhimurium) has shown strong adjuvant effects for a variety of vac...

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Autores principales: Pang, Shengmei, Wu, Wenwen, Liu, Qinfang, Zhu, Guoqiang, Duan, Qiangde
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9373361/
https://www.ncbi.nlm.nih.gov/pubmed/35953794
http://dx.doi.org/10.1186/s12917-022-03412-3
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author Pang, Shengmei
Wu, Wenwen
Liu, Qinfang
Zhu, Guoqiang
Duan, Qiangde
author_facet Pang, Shengmei
Wu, Wenwen
Liu, Qinfang
Zhu, Guoqiang
Duan, Qiangde
author_sort Pang, Shengmei
collection PubMed
description Bacterial flagellin is a potent powerful adjuvant, which exerts its adjuvant activity by activating the Toll-like receptor 5 (TLR5) signaling pathway to induce host pro-inflammatory responses. Flagellin of Salmonella typhimurium (S. typhimurium) has shown strong adjuvant effects for a variety of vaccine candidates, however, the adjuvanticity of different serotypes of Escherichia coli (E. coli) flagellin (FliC) is unclear. To explore the adjuvant activity of different serotypes of E. coli flagellin, FliC(H1), FliC(H7), and FliC(H19) recombinant flagellins were prokaryotically-expressed and purified. The adjuvanticity of three recombinant flagellins was evaluated by analyzing their abilities to induce the IL-8 production in human colorectal adenocarcinoma (Caco-2) cells and the immune responses to co-administrated FaeG antigen in mice. Sequence analysis showed that the N-and C-terminal regions are highly conserved, whereas the central region is hypervariable. The TLR5 recognized site is identical among these three serotypes of flagellins. Coomassie blue staining SDS-PAGE showed the molecular mass of FliC(H1), FliC(H7), and FliC(H19) recombinant flagellin are 66 kDa, 64 kDa, and 68 kDa, which can be recognized by anti-FliC(H1), FliC(H7), and FliC(H19) serum, respectively. Moreover, the flagellin serotypes induced similar levels of IL-8 and TNF-α production in Caco-2 cells, anti-FaeG specific IgG antibodies in mice, and IL-4 production in mice spleen cells. Our results indicated that E. coli flagellins can be an adjuvant for vaccine candidates and that different serotypes of E. coli flagellins possess identical adjuvant effects. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12917-022-03412-3.
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spelling pubmed-93733612022-08-13 Different serotypes of Escherichia coli flagellin exert identical adjuvant effects Pang, Shengmei Wu, Wenwen Liu, Qinfang Zhu, Guoqiang Duan, Qiangde BMC Vet Res Research Bacterial flagellin is a potent powerful adjuvant, which exerts its adjuvant activity by activating the Toll-like receptor 5 (TLR5) signaling pathway to induce host pro-inflammatory responses. Flagellin of Salmonella typhimurium (S. typhimurium) has shown strong adjuvant effects for a variety of vaccine candidates, however, the adjuvanticity of different serotypes of Escherichia coli (E. coli) flagellin (FliC) is unclear. To explore the adjuvant activity of different serotypes of E. coli flagellin, FliC(H1), FliC(H7), and FliC(H19) recombinant flagellins were prokaryotically-expressed and purified. The adjuvanticity of three recombinant flagellins was evaluated by analyzing their abilities to induce the IL-8 production in human colorectal adenocarcinoma (Caco-2) cells and the immune responses to co-administrated FaeG antigen in mice. Sequence analysis showed that the N-and C-terminal regions are highly conserved, whereas the central region is hypervariable. The TLR5 recognized site is identical among these three serotypes of flagellins. Coomassie blue staining SDS-PAGE showed the molecular mass of FliC(H1), FliC(H7), and FliC(H19) recombinant flagellin are 66 kDa, 64 kDa, and 68 kDa, which can be recognized by anti-FliC(H1), FliC(H7), and FliC(H19) serum, respectively. Moreover, the flagellin serotypes induced similar levels of IL-8 and TNF-α production in Caco-2 cells, anti-FaeG specific IgG antibodies in mice, and IL-4 production in mice spleen cells. Our results indicated that E. coli flagellins can be an adjuvant for vaccine candidates and that different serotypes of E. coli flagellins possess identical adjuvant effects. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12917-022-03412-3. BioMed Central 2022-08-12 /pmc/articles/PMC9373361/ /pubmed/35953794 http://dx.doi.org/10.1186/s12917-022-03412-3 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Pang, Shengmei
Wu, Wenwen
Liu, Qinfang
Zhu, Guoqiang
Duan, Qiangde
Different serotypes of Escherichia coli flagellin exert identical adjuvant effects
title Different serotypes of Escherichia coli flagellin exert identical adjuvant effects
title_full Different serotypes of Escherichia coli flagellin exert identical adjuvant effects
title_fullStr Different serotypes of Escherichia coli flagellin exert identical adjuvant effects
title_full_unstemmed Different serotypes of Escherichia coli flagellin exert identical adjuvant effects
title_short Different serotypes of Escherichia coli flagellin exert identical adjuvant effects
title_sort different serotypes of escherichia coli flagellin exert identical adjuvant effects
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9373361/
https://www.ncbi.nlm.nih.gov/pubmed/35953794
http://dx.doi.org/10.1186/s12917-022-03412-3
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