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Effects of hawthorn seed oil on plasma cholesterol and gut microbiota

BACKGROUND: Hypercholesterolemia and gut microbiota dysbiosis are associated with the risk of cardiovascular diseases. Hawthorn fruits has shown to be cardioprotective and hypocholesterolemic. However, no studies to date have studied the biological activity of hawthorn seed oil (HSO). The present st...

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Autores principales: Kwek, Erika, Yan, Chi, Ding, Huafang, Hao, Wangjun, He, Zouyan, Liu, Jianhui, Ma, Ka Ying, Zhu, Hanyue, Chen, Zhen-Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9373405/
https://www.ncbi.nlm.nih.gov/pubmed/35962418
http://dx.doi.org/10.1186/s12986-022-00690-4
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author Kwek, Erika
Yan, Chi
Ding, Huafang
Hao, Wangjun
He, Zouyan
Liu, Jianhui
Ma, Ka Ying
Zhu, Hanyue
Chen, Zhen-Yu
author_facet Kwek, Erika
Yan, Chi
Ding, Huafang
Hao, Wangjun
He, Zouyan
Liu, Jianhui
Ma, Ka Ying
Zhu, Hanyue
Chen, Zhen-Yu
author_sort Kwek, Erika
collection PubMed
description BACKGROUND: Hypercholesterolemia and gut microbiota dysbiosis are associated with the risk of cardiovascular diseases. Hawthorn fruits has shown to be cardioprotective and hypocholesterolemic. However, no studies to date have studied the biological activity of hawthorn seed oil (HSO). The present study was to investigate if HSO could favourably reduce plasma cholesterol and modulate gut microbiota in hypercholesterolemia hamsters. METHODS: Golden Syrian hamsters (age, 8 weeks) were randomly divided into five groups (n = 8, each) and fed one of the following five diets, namely a non-cholesterol diet, a high cholesterol diet containing 0.15% cholesterol (HCD); a HCD diet with addition of 4.75% HSO (LHSO), a HCD diet with addition of 9.5% HSO (HHSO), a HCD diet with addition of 0.50% cholestyramine as positive control diet. After 6-week dietary intervention, plasma lipids, inflammatory markers, atherosclerosis plaque, hepatic and fecal lipids were quantified. Microbiota in fresh feces were analysed by sequencing 16S rRNA genes, while RT-PCR and Western blot analyses were employed to quantify the expression of genes involved in cholesterol homeostasis. RESULTS: HSO at a dose of 9.5% HSO could decrease plasma cholesterol and non-HDL-cholesterol by 15%. Additionally, both HSO experimental groups also suppressed mRNA of 3-Hydroxy-3-Methylglutaryl-CoA Reductase (HMG-CoA-R). Supplementation of HSO at 4.75% could significantly increase the excretion of fecal acidic sterols, accompanied by elevation of short-chain fatty acid levels in feces. The analyses of gut microbiome indicated that HSO supplementation could selectively alter the genera abundance of gut bacteria that were correlated with cholesterol metabolism including unclassified_f__Christensenellaceae, Ruminococcaceae_NK4A214_ group, norank_o_Gastranaerophilales, Faecalibaculum, Peptococcus, norank_f__Clostridiales_vadinBB60_group and Ruminococcus_2. CONCLUSIONS: HSO supplementation was able to decrease plasma cholesterol by favourably modulating gut microbiota composition and gut-derived metabolites associated with cholesterol regulation. GRAPHICAL ABSTRACT: [Image: see text]
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spelling pubmed-93734052022-08-13 Effects of hawthorn seed oil on plasma cholesterol and gut microbiota Kwek, Erika Yan, Chi Ding, Huafang Hao, Wangjun He, Zouyan Liu, Jianhui Ma, Ka Ying Zhu, Hanyue Chen, Zhen-Yu Nutr Metab (Lond) Research BACKGROUND: Hypercholesterolemia and gut microbiota dysbiosis are associated with the risk of cardiovascular diseases. Hawthorn fruits has shown to be cardioprotective and hypocholesterolemic. However, no studies to date have studied the biological activity of hawthorn seed oil (HSO). The present study was to investigate if HSO could favourably reduce plasma cholesterol and modulate gut microbiota in hypercholesterolemia hamsters. METHODS: Golden Syrian hamsters (age, 8 weeks) were randomly divided into five groups (n = 8, each) and fed one of the following five diets, namely a non-cholesterol diet, a high cholesterol diet containing 0.15% cholesterol (HCD); a HCD diet with addition of 4.75% HSO (LHSO), a HCD diet with addition of 9.5% HSO (HHSO), a HCD diet with addition of 0.50% cholestyramine as positive control diet. After 6-week dietary intervention, plasma lipids, inflammatory markers, atherosclerosis plaque, hepatic and fecal lipids were quantified. Microbiota in fresh feces were analysed by sequencing 16S rRNA genes, while RT-PCR and Western blot analyses were employed to quantify the expression of genes involved in cholesterol homeostasis. RESULTS: HSO at a dose of 9.5% HSO could decrease plasma cholesterol and non-HDL-cholesterol by 15%. Additionally, both HSO experimental groups also suppressed mRNA of 3-Hydroxy-3-Methylglutaryl-CoA Reductase (HMG-CoA-R). Supplementation of HSO at 4.75% could significantly increase the excretion of fecal acidic sterols, accompanied by elevation of short-chain fatty acid levels in feces. The analyses of gut microbiome indicated that HSO supplementation could selectively alter the genera abundance of gut bacteria that were correlated with cholesterol metabolism including unclassified_f__Christensenellaceae, Ruminococcaceae_NK4A214_ group, norank_o_Gastranaerophilales, Faecalibaculum, Peptococcus, norank_f__Clostridiales_vadinBB60_group and Ruminococcus_2. CONCLUSIONS: HSO supplementation was able to decrease plasma cholesterol by favourably modulating gut microbiota composition and gut-derived metabolites associated with cholesterol regulation. GRAPHICAL ABSTRACT: [Image: see text] BioMed Central 2022-08-12 /pmc/articles/PMC9373405/ /pubmed/35962418 http://dx.doi.org/10.1186/s12986-022-00690-4 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Kwek, Erika
Yan, Chi
Ding, Huafang
Hao, Wangjun
He, Zouyan
Liu, Jianhui
Ma, Ka Ying
Zhu, Hanyue
Chen, Zhen-Yu
Effects of hawthorn seed oil on plasma cholesterol and gut microbiota
title Effects of hawthorn seed oil on plasma cholesterol and gut microbiota
title_full Effects of hawthorn seed oil on plasma cholesterol and gut microbiota
title_fullStr Effects of hawthorn seed oil on plasma cholesterol and gut microbiota
title_full_unstemmed Effects of hawthorn seed oil on plasma cholesterol and gut microbiota
title_short Effects of hawthorn seed oil on plasma cholesterol and gut microbiota
title_sort effects of hawthorn seed oil on plasma cholesterol and gut microbiota
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9373405/
https://www.ncbi.nlm.nih.gov/pubmed/35962418
http://dx.doi.org/10.1186/s12986-022-00690-4
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