Comprehensive analysis of RNA m6A methylation in pressure overload-induced cardiac hypertrophy

AIM: To analyze and compare the mRNA N(6)-methyladenosine modifications in transverse aortic constriction induced mice hearts and normal mice hearts. MATERIALS AND METHODS: Colorimetric quantification was used to probe the changes in m(6)A modifications in the total RNA. The expression of m(6)A-related enzymes was analyzed via qRT-PCR and western blotting. RNA-seq and MeRIP-seq were performed to identify genes with differences in m(6)A modifications or expression in the transcriptome profile. RESULTS: Compared with the control group, the TAC group exhibited higher m(6)A methylation levels. FTO and WTAP were downregulated after TAC, while METTL3 was significantly downregulated at the protein level. MeRIP-seq revealed that 1179 m(6)A peaks were upmethylated and 733 m(6)A peaks were downmethylated, and biological analysis of these genes exhibited a strong relationship with heart function. CONCLUSION: Our findings provide novel information regarding m(6)A modification and gene expression changes in cardiac hypertrophy, which may be fundamental for further research. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-022-08833-w.