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Protection Evaluation of a Five-Gene-Deleted African Swine Fever Virus Vaccine Candidate Against Homologous Challenge

African swine fever virus (ASFV) represents a serious threat to the global swine industry, and there are no safe or commercially available vaccines. Previous studies have demonstrated that inactivated vaccines do not provide sufficient protection against ASFV and that attenuated vaccines are effecti...

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Autores principales: Xie, Zhenhua, Liu, Yingnan, Di, Dongdong, Liu, Jingyi, Gong, Lang, Chen, Zongyan, Li, Yao, Yu, Wanqi, Lv, Lu, Zhong, Qiuping, Song, Yingying, Liao, Xinxin, Song, Qingqing, Wang, Heng, Chen, Hongjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9374035/
https://www.ncbi.nlm.nih.gov/pubmed/35966648
http://dx.doi.org/10.3389/fmicb.2022.902932
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author Xie, Zhenhua
Liu, Yingnan
Di, Dongdong
Liu, Jingyi
Gong, Lang
Chen, Zongyan
Li, Yao
Yu, Wanqi
Lv, Lu
Zhong, Qiuping
Song, Yingying
Liao, Xinxin
Song, Qingqing
Wang, Heng
Chen, Hongjun
author_facet Xie, Zhenhua
Liu, Yingnan
Di, Dongdong
Liu, Jingyi
Gong, Lang
Chen, Zongyan
Li, Yao
Yu, Wanqi
Lv, Lu
Zhong, Qiuping
Song, Yingying
Liao, Xinxin
Song, Qingqing
Wang, Heng
Chen, Hongjun
author_sort Xie, Zhenhua
collection PubMed
description African swine fever virus (ASFV) represents a serious threat to the global swine industry, and there are no safe or commercially available vaccines. Previous studies have demonstrated that inactivated vaccines do not provide sufficient protection against ASFV and that attenuated vaccines are effective, but raise safety concerns. Here, we first constructed a deletion mutant in which EP153R and EP402R gene clusters were knocked out. Based on the deletion mutant, a further deletion from the MGF_360-12L, MGF_360-13L to MGF_360-14L genes was obtained. The five-genes knockout virus was designated as ASFV-ΔECM3. To investigate the efficacy and safety of the ASFV-ΔECM3 virus as a vaccine candidate, the evaluation of the virus was subsequently carried out in pigs. The results showed that the ASFV-ΔECM3 virus could induce homologous protection against the parental isolate, and no significant clinical signs or viremia were observed. These results show that the contiguous deletion mutant, ASFV-ΔECM3 encompassing the EP153R/EP402R and MGF_360-12L/13L/14L genes, could be a potential live-attenuated vaccine candidate for the prevention of ASFV infection.
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spelling pubmed-93740352022-08-13 Protection Evaluation of a Five-Gene-Deleted African Swine Fever Virus Vaccine Candidate Against Homologous Challenge Xie, Zhenhua Liu, Yingnan Di, Dongdong Liu, Jingyi Gong, Lang Chen, Zongyan Li, Yao Yu, Wanqi Lv, Lu Zhong, Qiuping Song, Yingying Liao, Xinxin Song, Qingqing Wang, Heng Chen, Hongjun Front Microbiol Microbiology African swine fever virus (ASFV) represents a serious threat to the global swine industry, and there are no safe or commercially available vaccines. Previous studies have demonstrated that inactivated vaccines do not provide sufficient protection against ASFV and that attenuated vaccines are effective, but raise safety concerns. Here, we first constructed a deletion mutant in which EP153R and EP402R gene clusters were knocked out. Based on the deletion mutant, a further deletion from the MGF_360-12L, MGF_360-13L to MGF_360-14L genes was obtained. The five-genes knockout virus was designated as ASFV-ΔECM3. To investigate the efficacy and safety of the ASFV-ΔECM3 virus as a vaccine candidate, the evaluation of the virus was subsequently carried out in pigs. The results showed that the ASFV-ΔECM3 virus could induce homologous protection against the parental isolate, and no significant clinical signs or viremia were observed. These results show that the contiguous deletion mutant, ASFV-ΔECM3 encompassing the EP153R/EP402R and MGF_360-12L/13L/14L genes, could be a potential live-attenuated vaccine candidate for the prevention of ASFV infection. Frontiers Media S.A. 2022-07-29 /pmc/articles/PMC9374035/ /pubmed/35966648 http://dx.doi.org/10.3389/fmicb.2022.902932 Text en Copyright © 2022 Xie, Liu, Di, Liu, Gong, Chen, Li, Yu, Lv, Zhong, Song, Liao, Song, Wang and Chen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Xie, Zhenhua
Liu, Yingnan
Di, Dongdong
Liu, Jingyi
Gong, Lang
Chen, Zongyan
Li, Yao
Yu, Wanqi
Lv, Lu
Zhong, Qiuping
Song, Yingying
Liao, Xinxin
Song, Qingqing
Wang, Heng
Chen, Hongjun
Protection Evaluation of a Five-Gene-Deleted African Swine Fever Virus Vaccine Candidate Against Homologous Challenge
title Protection Evaluation of a Five-Gene-Deleted African Swine Fever Virus Vaccine Candidate Against Homologous Challenge
title_full Protection Evaluation of a Five-Gene-Deleted African Swine Fever Virus Vaccine Candidate Against Homologous Challenge
title_fullStr Protection Evaluation of a Five-Gene-Deleted African Swine Fever Virus Vaccine Candidate Against Homologous Challenge
title_full_unstemmed Protection Evaluation of a Five-Gene-Deleted African Swine Fever Virus Vaccine Candidate Against Homologous Challenge
title_short Protection Evaluation of a Five-Gene-Deleted African Swine Fever Virus Vaccine Candidate Against Homologous Challenge
title_sort protection evaluation of a five-gene-deleted african swine fever virus vaccine candidate against homologous challenge
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9374035/
https://www.ncbi.nlm.nih.gov/pubmed/35966648
http://dx.doi.org/10.3389/fmicb.2022.902932
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