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Host and microbiome features of secondary infections in lethal covid-19
Secondary infections contribute significantly to covid-19 mortality but driving factors remain poorly understood. Autopsies of 20 covid-19 cases and 14 controls from the first pandemic wave complemented with microbial cultivation and RNA-seq from lung tissues enabled description of major organ patho...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9374491/ https://www.ncbi.nlm.nih.gov/pubmed/35992303 http://dx.doi.org/10.1016/j.isci.2022.104926 |
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author | Zacharias, Martin Kashofer, Karl Wurm, Philipp Regitnig, Peter Schütte, Moritz Neger, Margit Ehmann, Sandra Marsh, Leigh M. Kwapiszewska, Grazyna Loibner, Martina Birnhuber, Anna Leitner, Eva Thüringer, Andrea Winter, Elke Sauer, Stefan Pollheimer, Marion J. Vagena, Fotini R. Lackner, Carolin Jelusic, Barbara Ogilvie, Lesley Durdevic, Marija Timmermann, Bernd Lehrach, Hans Zatloukal, Kurt Gorkiewicz, Gregor |
author_facet | Zacharias, Martin Kashofer, Karl Wurm, Philipp Regitnig, Peter Schütte, Moritz Neger, Margit Ehmann, Sandra Marsh, Leigh M. Kwapiszewska, Grazyna Loibner, Martina Birnhuber, Anna Leitner, Eva Thüringer, Andrea Winter, Elke Sauer, Stefan Pollheimer, Marion J. Vagena, Fotini R. Lackner, Carolin Jelusic, Barbara Ogilvie, Lesley Durdevic, Marija Timmermann, Bernd Lehrach, Hans Zatloukal, Kurt Gorkiewicz, Gregor |
author_sort | Zacharias, Martin |
collection | PubMed |
description | Secondary infections contribute significantly to covid-19 mortality but driving factors remain poorly understood. Autopsies of 20 covid-19 cases and 14 controls from the first pandemic wave complemented with microbial cultivation and RNA-seq from lung tissues enabled description of major organ pathologies and specification of secondary infections. Lethal covid-19 segregated into two main death causes with either dominant diffuse alveolar damage (DAD) or secondary pneumonias. The lung microbiome in covid-19 showed a reduced biodiversity and increased prototypical bacterial and fungal pathogens in cases of secondary pneumonias. RNA-seq distinctly mirrored death causes and stratified DAD cases into subgroups with differing cellular compositions identifying myeloid cells, macrophages and complement C1q as strong separating factors suggesting a pathophysiological link. Together with a prominent induction of inhibitory immune-checkpoints our study highlights profound alterations of the lung immunity in covid-19 wherein a reduced antimicrobial defense likely drives development of secondary infections on top of SARS-CoV-2 infection. |
format | Online Article Text |
id | pubmed-9374491 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-93744912022-08-15 Host and microbiome features of secondary infections in lethal covid-19 Zacharias, Martin Kashofer, Karl Wurm, Philipp Regitnig, Peter Schütte, Moritz Neger, Margit Ehmann, Sandra Marsh, Leigh M. Kwapiszewska, Grazyna Loibner, Martina Birnhuber, Anna Leitner, Eva Thüringer, Andrea Winter, Elke Sauer, Stefan Pollheimer, Marion J. Vagena, Fotini R. Lackner, Carolin Jelusic, Barbara Ogilvie, Lesley Durdevic, Marija Timmermann, Bernd Lehrach, Hans Zatloukal, Kurt Gorkiewicz, Gregor iScience Article Secondary infections contribute significantly to covid-19 mortality but driving factors remain poorly understood. Autopsies of 20 covid-19 cases and 14 controls from the first pandemic wave complemented with microbial cultivation and RNA-seq from lung tissues enabled description of major organ pathologies and specification of secondary infections. Lethal covid-19 segregated into two main death causes with either dominant diffuse alveolar damage (DAD) or secondary pneumonias. The lung microbiome in covid-19 showed a reduced biodiversity and increased prototypical bacterial and fungal pathogens in cases of secondary pneumonias. RNA-seq distinctly mirrored death causes and stratified DAD cases into subgroups with differing cellular compositions identifying myeloid cells, macrophages and complement C1q as strong separating factors suggesting a pathophysiological link. Together with a prominent induction of inhibitory immune-checkpoints our study highlights profound alterations of the lung immunity in covid-19 wherein a reduced antimicrobial defense likely drives development of secondary infections on top of SARS-CoV-2 infection. Elsevier 2022-08-13 /pmc/articles/PMC9374491/ /pubmed/35992303 http://dx.doi.org/10.1016/j.isci.2022.104926 Text en © 2022 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zacharias, Martin Kashofer, Karl Wurm, Philipp Regitnig, Peter Schütte, Moritz Neger, Margit Ehmann, Sandra Marsh, Leigh M. Kwapiszewska, Grazyna Loibner, Martina Birnhuber, Anna Leitner, Eva Thüringer, Andrea Winter, Elke Sauer, Stefan Pollheimer, Marion J. Vagena, Fotini R. Lackner, Carolin Jelusic, Barbara Ogilvie, Lesley Durdevic, Marija Timmermann, Bernd Lehrach, Hans Zatloukal, Kurt Gorkiewicz, Gregor Host and microbiome features of secondary infections in lethal covid-19 |
title | Host and microbiome features of secondary infections in lethal covid-19 |
title_full | Host and microbiome features of secondary infections in lethal covid-19 |
title_fullStr | Host and microbiome features of secondary infections in lethal covid-19 |
title_full_unstemmed | Host and microbiome features of secondary infections in lethal covid-19 |
title_short | Host and microbiome features of secondary infections in lethal covid-19 |
title_sort | host and microbiome features of secondary infections in lethal covid-19 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9374491/ https://www.ncbi.nlm.nih.gov/pubmed/35992303 http://dx.doi.org/10.1016/j.isci.2022.104926 |
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