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Global transcriptional and circadian regulation in a halotolerant cyanobacterium Halothece sp. PCC7418

Substantial evidence has been accumulated about the molecular basis underlying halotolerance; however, insights into the regulatory networks for relevant genes and mechanisms of their interplay remain elusive. Here, we present a comprehensive transcriptome investigation, using RNA sequencing, of spe...

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Autores principales: Waditee-Sirisattha, Rungaroon, Ito, Hiroshi, Kageyama, Hakuto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9374696/
https://www.ncbi.nlm.nih.gov/pubmed/35962002
http://dx.doi.org/10.1038/s41598-022-17406-6
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author Waditee-Sirisattha, Rungaroon
Ito, Hiroshi
Kageyama, Hakuto
author_facet Waditee-Sirisattha, Rungaroon
Ito, Hiroshi
Kageyama, Hakuto
author_sort Waditee-Sirisattha, Rungaroon
collection PubMed
description Substantial evidence has been accumulated about the molecular basis underlying halotolerance; however, insights into the regulatory networks for relevant genes and mechanisms of their interplay remain elusive. Here, we present a comprehensive transcriptome investigation, using RNA sequencing, of specific metabolic pathways and networks in a halotolerant cyanobacterium, Halothece sp. PCC7418, including the circadian rhythm profile. Dissecting the transcriptome presented the intracellular regulation of gene expressions, which was linked with ion homeostasis, protein homeostasis, biosynthesis of compatible solutes, and signal transduction, for adaptations to high-salinity environments. The efficient production and distribution of energy were also implicated in this acclimation process. Furthermore, we found that high-salinity environments had a dramatic effect on the global transcriptional expression regulated by the circadian clock. Our findings can provide a comprehensive transcriptome for elucidating the molecular mechanisms underlying halotolerance in cyanobacteria.
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spelling pubmed-93746962022-08-14 Global transcriptional and circadian regulation in a halotolerant cyanobacterium Halothece sp. PCC7418 Waditee-Sirisattha, Rungaroon Ito, Hiroshi Kageyama, Hakuto Sci Rep Article Substantial evidence has been accumulated about the molecular basis underlying halotolerance; however, insights into the regulatory networks for relevant genes and mechanisms of their interplay remain elusive. Here, we present a comprehensive transcriptome investigation, using RNA sequencing, of specific metabolic pathways and networks in a halotolerant cyanobacterium, Halothece sp. PCC7418, including the circadian rhythm profile. Dissecting the transcriptome presented the intracellular regulation of gene expressions, which was linked with ion homeostasis, protein homeostasis, biosynthesis of compatible solutes, and signal transduction, for adaptations to high-salinity environments. The efficient production and distribution of energy were also implicated in this acclimation process. Furthermore, we found that high-salinity environments had a dramatic effect on the global transcriptional expression regulated by the circadian clock. Our findings can provide a comprehensive transcriptome for elucidating the molecular mechanisms underlying halotolerance in cyanobacteria. Nature Publishing Group UK 2022-08-12 /pmc/articles/PMC9374696/ /pubmed/35962002 http://dx.doi.org/10.1038/s41598-022-17406-6 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Waditee-Sirisattha, Rungaroon
Ito, Hiroshi
Kageyama, Hakuto
Global transcriptional and circadian regulation in a halotolerant cyanobacterium Halothece sp. PCC7418
title Global transcriptional and circadian regulation in a halotolerant cyanobacterium Halothece sp. PCC7418
title_full Global transcriptional and circadian regulation in a halotolerant cyanobacterium Halothece sp. PCC7418
title_fullStr Global transcriptional and circadian regulation in a halotolerant cyanobacterium Halothece sp. PCC7418
title_full_unstemmed Global transcriptional and circadian regulation in a halotolerant cyanobacterium Halothece sp. PCC7418
title_short Global transcriptional and circadian regulation in a halotolerant cyanobacterium Halothece sp. PCC7418
title_sort global transcriptional and circadian regulation in a halotolerant cyanobacterium halothece sp. pcc7418
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9374696/
https://www.ncbi.nlm.nih.gov/pubmed/35962002
http://dx.doi.org/10.1038/s41598-022-17406-6
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