Generation of a CRISPR activation mouse that enables modelling of aggressive lymphoma and interrogation of venetoclax resistance

CRISPR technologies have advanced cancer modelling in mice, but CRISPR activation (CRISPRa) methods have not been exploited in this context. We establish a CRISPRa mouse (dCas9a-SAM(KI)) for inducing gene expression in vivo and in vitro. Using dCas9a-SAM(KI) primary lymphocytes, we induce B cell res...

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Detalles Bibliográficos
Autores principales: Deng, Yexuan, Diepstraten, Sarah T., Potts, Margaret A., Giner, Göknur, Trezise, Stephanie, Ng, Ashley P., Healey, Gerry, Kane, Serena R., Cooray, Amali, Behrens, Kira, Heidersbach, Amy, Kueh, Andrew J., Pal, Martin, Wilcox, Stephen, Tai, Lin, Alexander, Warren S., Visvader, Jane E., Nutt, Stephen L., Strasser, Andreas, Haley, Benjamin, Zhao, Quan, Kelly, Gemma L., Herold, Marco J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9374748/
https://www.ncbi.nlm.nih.gov/pubmed/35961968
http://dx.doi.org/10.1038/s41467-022-32485-9

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