FTO promotes clear cell renal cell carcinoma progression via upregulation of PDK1 through an m(6)A dependent pathway

FTO, as an m(6)A mRNA demethylase, is involved in various cancers. However, the role of FTO in clear cell renal cell carcinoma (ccRCC) remains unclear. In the present study, we discovered FTO is upregulated in ccRCC. Functionally, knockdown of FTO significantly impairs the proliferation and migratio...

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Autores principales: Shen, Haixiang, Ying, Yufan, Ma, Xueyou, Xie, Haiyun, Chen, Shiming, Sun, Jiazhu, Liu, Zixiang, Wen, Chao, Yang, Zitong, Wang, Xiao, Xu, Mingjie, Luo, Jindan, Liu, Ben, Li, Jiangfeng, Zheng, Xiangyi, Xie, Liping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9374762/
https://www.ncbi.nlm.nih.gov/pubmed/35961973
http://dx.doi.org/10.1038/s41420-022-01151-w
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author Shen, Haixiang
Ying, Yufan
Ma, Xueyou
Xie, Haiyun
Chen, Shiming
Sun, Jiazhu
Liu, Zixiang
Wen, Chao
Yang, Zitong
Wang, Xiao
Xu, Mingjie
Luo, Jindan
Liu, Ben
Li, Jiangfeng
Zheng, Xiangyi
Xie, Liping
author_facet Shen, Haixiang
Ying, Yufan
Ma, Xueyou
Xie, Haiyun
Chen, Shiming
Sun, Jiazhu
Liu, Zixiang
Wen, Chao
Yang, Zitong
Wang, Xiao
Xu, Mingjie
Luo, Jindan
Liu, Ben
Li, Jiangfeng
Zheng, Xiangyi
Xie, Liping
author_sort Shen, Haixiang
collection PubMed
description FTO, as an m(6)A mRNA demethylase, is involved in various cancers. However, the role of FTO in clear cell renal cell carcinoma (ccRCC) remains unclear. In the present study, we discovered FTO is upregulated in ccRCC. Functionally, knockdown of FTO significantly impairs the proliferation and migration ability of ccRCC cells. Mechanistically, our data suggest FTO promotes the proliferation and migration of ccRCC through preventing degradation of PDK1 mRNA induced by YTHDF2 in an m(6)A-dependent mechanism. Overall, our results identify the protumorigenic role of FTO through the m(6)A/YTHDF2/PDK1 pathway, which could be a promising therapeutic target for ccRCC.
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spelling pubmed-93747622022-08-14 FTO promotes clear cell renal cell carcinoma progression via upregulation of PDK1 through an m(6)A dependent pathway Shen, Haixiang Ying, Yufan Ma, Xueyou Xie, Haiyun Chen, Shiming Sun, Jiazhu Liu, Zixiang Wen, Chao Yang, Zitong Wang, Xiao Xu, Mingjie Luo, Jindan Liu, Ben Li, Jiangfeng Zheng, Xiangyi Xie, Liping Cell Death Discov Article FTO, as an m(6)A mRNA demethylase, is involved in various cancers. However, the role of FTO in clear cell renal cell carcinoma (ccRCC) remains unclear. In the present study, we discovered FTO is upregulated in ccRCC. Functionally, knockdown of FTO significantly impairs the proliferation and migration ability of ccRCC cells. Mechanistically, our data suggest FTO promotes the proliferation and migration of ccRCC through preventing degradation of PDK1 mRNA induced by YTHDF2 in an m(6)A-dependent mechanism. Overall, our results identify the protumorigenic role of FTO through the m(6)A/YTHDF2/PDK1 pathway, which could be a promising therapeutic target for ccRCC. Nature Publishing Group UK 2022-08-12 /pmc/articles/PMC9374762/ /pubmed/35961973 http://dx.doi.org/10.1038/s41420-022-01151-w Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Shen, Haixiang
Ying, Yufan
Ma, Xueyou
Xie, Haiyun
Chen, Shiming
Sun, Jiazhu
Liu, Zixiang
Wen, Chao
Yang, Zitong
Wang, Xiao
Xu, Mingjie
Luo, Jindan
Liu, Ben
Li, Jiangfeng
Zheng, Xiangyi
Xie, Liping
FTO promotes clear cell renal cell carcinoma progression via upregulation of PDK1 through an m(6)A dependent pathway
title FTO promotes clear cell renal cell carcinoma progression via upregulation of PDK1 through an m(6)A dependent pathway
title_full FTO promotes clear cell renal cell carcinoma progression via upregulation of PDK1 through an m(6)A dependent pathway
title_fullStr FTO promotes clear cell renal cell carcinoma progression via upregulation of PDK1 through an m(6)A dependent pathway
title_full_unstemmed FTO promotes clear cell renal cell carcinoma progression via upregulation of PDK1 through an m(6)A dependent pathway
title_short FTO promotes clear cell renal cell carcinoma progression via upregulation of PDK1 through an m(6)A dependent pathway
title_sort fto promotes clear cell renal cell carcinoma progression via upregulation of pdk1 through an m(6)a dependent pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9374762/
https://www.ncbi.nlm.nih.gov/pubmed/35961973
http://dx.doi.org/10.1038/s41420-022-01151-w
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