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Molecular identification of lactic acid bacteria SR6 strain and evaluation of its activity as an anticancer in T47D cell line

BACKGROUND AND AIM: Breast cancer is the most common type of cancer in women because it attacks the productive age. Preliminary studies showed that lactic acid bacteria (LAB) strain SR6 from the Bali cattle colon has the potential to act as a superior probiotic. It is also assumed that its bacterioc...

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Autores principales: Swacita, Ida Bagus Ngurah, Suardana, I. Wayan, Sudisma, I. Gusti Ngurah, Wihadmadyatami, Hevi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Veterinary World 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9375210/
https://www.ncbi.nlm.nih.gov/pubmed/35993063
http://dx.doi.org/10.14202/vetworld.2022.1583-1588
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author Swacita, Ida Bagus Ngurah
Suardana, I. Wayan
Sudisma, I. Gusti Ngurah
Wihadmadyatami, Hevi
author_facet Swacita, Ida Bagus Ngurah
Suardana, I. Wayan
Sudisma, I. Gusti Ngurah
Wihadmadyatami, Hevi
author_sort Swacita, Ida Bagus Ngurah
collection PubMed
description BACKGROUND AND AIM: Breast cancer is the most common type of cancer in women because it attacks the productive age. Preliminary studies showed that lactic acid bacteria (LAB) strain SR6 from the Bali cattle colon has the potential to act as a superior probiotic. It is also assumed that its bacteriocin structure is specific and has a strong relationship with the specificity of the ligand and its biological activity at a receptor. Therefore, this study aims to assess the use of local LAB strains, which produce bacteriocins as anticancer agents, as well as to identify the bacteria as potent producers molecularly. MATERIALS AND METHODS: The study was initiated by cultivating LAB SR6 strain from stock isolates on De Man, Rogosa, and Sharpe (Oxoid, CM 0369, England) broth media. It was then confirmed molecularly through analysis of the 16S ribosomal ribonucleic acid gene. Subsequently, its anticancer activity was tested by assessing the cytotoxic activity in T47D cell culture using the 3-(4, 5 dimetiltiazol-2-yl)-2.5-diphenyl tetrazolium bromide (Invitrogen M6494, US) method. RESULTS: The results showed that the LAB strain SR6 was identified molecularly as Pediococcus pentosaceus. Furthermore, it had a toxic effect on T47D cells, which was indicated by the number of deaths after treatment with the extracellular protein of the strain, especially at the 50% total cell volume level. CONCLUSION: Based on the toxic effect of the strain on human T47D cells, the LAB SR6 isolate, which was identified as P. pentosaceus has the potential to be developed as a good anticancer drug against breast cancer. However, there is a need to carry out an integrated study to fully explore the suitability of bacteriocins as in vivo therapeutics against the disease completely.
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spelling pubmed-93752102022-08-19 Molecular identification of lactic acid bacteria SR6 strain and evaluation of its activity as an anticancer in T47D cell line Swacita, Ida Bagus Ngurah Suardana, I. Wayan Sudisma, I. Gusti Ngurah Wihadmadyatami, Hevi Vet World Research Article BACKGROUND AND AIM: Breast cancer is the most common type of cancer in women because it attacks the productive age. Preliminary studies showed that lactic acid bacteria (LAB) strain SR6 from the Bali cattle colon has the potential to act as a superior probiotic. It is also assumed that its bacteriocin structure is specific and has a strong relationship with the specificity of the ligand and its biological activity at a receptor. Therefore, this study aims to assess the use of local LAB strains, which produce bacteriocins as anticancer agents, as well as to identify the bacteria as potent producers molecularly. MATERIALS AND METHODS: The study was initiated by cultivating LAB SR6 strain from stock isolates on De Man, Rogosa, and Sharpe (Oxoid, CM 0369, England) broth media. It was then confirmed molecularly through analysis of the 16S ribosomal ribonucleic acid gene. Subsequently, its anticancer activity was tested by assessing the cytotoxic activity in T47D cell culture using the 3-(4, 5 dimetiltiazol-2-yl)-2.5-diphenyl tetrazolium bromide (Invitrogen M6494, US) method. RESULTS: The results showed that the LAB strain SR6 was identified molecularly as Pediococcus pentosaceus. Furthermore, it had a toxic effect on T47D cells, which was indicated by the number of deaths after treatment with the extracellular protein of the strain, especially at the 50% total cell volume level. CONCLUSION: Based on the toxic effect of the strain on human T47D cells, the LAB SR6 isolate, which was identified as P. pentosaceus has the potential to be developed as a good anticancer drug against breast cancer. However, there is a need to carry out an integrated study to fully explore the suitability of bacteriocins as in vivo therapeutics against the disease completely. Veterinary World 2022-06 2022-06-29 /pmc/articles/PMC9375210/ /pubmed/35993063 http://dx.doi.org/10.14202/vetworld.2022.1583-1588 Text en Copyright: © Swacita, et al. https://creativecommons.org/licenses/by/4.0/Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Swacita, Ida Bagus Ngurah
Suardana, I. Wayan
Sudisma, I. Gusti Ngurah
Wihadmadyatami, Hevi
Molecular identification of lactic acid bacteria SR6 strain and evaluation of its activity as an anticancer in T47D cell line
title Molecular identification of lactic acid bacteria SR6 strain and evaluation of its activity as an anticancer in T47D cell line
title_full Molecular identification of lactic acid bacteria SR6 strain and evaluation of its activity as an anticancer in T47D cell line
title_fullStr Molecular identification of lactic acid bacteria SR6 strain and evaluation of its activity as an anticancer in T47D cell line
title_full_unstemmed Molecular identification of lactic acid bacteria SR6 strain and evaluation of its activity as an anticancer in T47D cell line
title_short Molecular identification of lactic acid bacteria SR6 strain and evaluation of its activity as an anticancer in T47D cell line
title_sort molecular identification of lactic acid bacteria sr6 strain and evaluation of its activity as an anticancer in t47d cell line
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9375210/
https://www.ncbi.nlm.nih.gov/pubmed/35993063
http://dx.doi.org/10.14202/vetworld.2022.1583-1588
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