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Expression and Purification of Porcine Rotavirus Structural Proteins in Silkworm Larvae as a Vaccine Candidate

In this study, silkworm larvae were used for expression of porcine rotavirus A (KS14 strain) inner capsid protein, VP6, and outer capsid protein, VP7. Initially, VP6 was fused with Strep-tag II and FLAG-tag (T-VP6), and T-VP6 was fused further with the signal peptide of Bombyx mori 30k6G protein (30...

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Detalles Bibliográficos
Autores principales: Kato, Tatsuya, Kakuta, Tatsuki, Yonezuka, Ami, Sekiguchi, Tomofumi, Machida, Yuki, Xu, Jian, Suzuki, Tohru, Park, Enoch Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9376036/
https://www.ncbi.nlm.nih.gov/pubmed/35963985
http://dx.doi.org/10.1007/s12033-022-00548-3

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