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A mid-infrared lab-on-a-chip for dynamic reaction monitoring

Mid-infrared spectroscopy is a sensitive and selective technique for probing molecules in the gas or liquid phase. Investigating chemical reactions in bio-medical applications such as drug production is recently gaining particular interest. However, monitoring dynamic processes in liquids is commonl...

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Autores principales: Hinkov, Borislav, Pilat, Florian, Lux, Laurin, Souza, Patricia L., David, Mauro, Schwaighofer, Andreas, Ristanić, Daniela, Schwarz, Benedikt, Detz, Hermann, Andrews, Aaron M., Lendl, Bernhard, Strasser, Gottfried
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9376098/
https://www.ncbi.nlm.nih.gov/pubmed/35963870
http://dx.doi.org/10.1038/s41467-022-32417-7
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author Hinkov, Borislav
Pilat, Florian
Lux, Laurin
Souza, Patricia L.
David, Mauro
Schwaighofer, Andreas
Ristanić, Daniela
Schwarz, Benedikt
Detz, Hermann
Andrews, Aaron M.
Lendl, Bernhard
Strasser, Gottfried
author_facet Hinkov, Borislav
Pilat, Florian
Lux, Laurin
Souza, Patricia L.
David, Mauro
Schwaighofer, Andreas
Ristanić, Daniela
Schwarz, Benedikt
Detz, Hermann
Andrews, Aaron M.
Lendl, Bernhard
Strasser, Gottfried
author_sort Hinkov, Borislav
collection PubMed
description Mid-infrared spectroscopy is a sensitive and selective technique for probing molecules in the gas or liquid phase. Investigating chemical reactions in bio-medical applications such as drug production is recently gaining particular interest. However, monitoring dynamic processes in liquids is commonly limited to bulky systems and thus requires time-consuming offline analytics. In this work, we show a next-generation, fully-integrated and robust chip-scale sensor for online measurements of molecule dynamics in a liquid solution. Our fingertip-sized device utilizes quantum cascade technology, combining the emitter, sensing section and detector on a single chip. This enables real-time measurements probing only microliter amounts of analyte in an in situ configuration. We demonstrate time-resolved device operation by analyzing temperature-induced conformational changes of the model protein bovine serum albumin in heavy water. Quantitative measurements reveal excellent performance characteristics in terms of sensor linearity, wide coverage of concentrations, extending from 0.075 mg ml(−1) to 92 mg ml(−1) and a 55-times higher absorbance than state-of-the-art bulky and offline reference systems.
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spelling pubmed-93760982022-08-15 A mid-infrared lab-on-a-chip for dynamic reaction monitoring Hinkov, Borislav Pilat, Florian Lux, Laurin Souza, Patricia L. David, Mauro Schwaighofer, Andreas Ristanić, Daniela Schwarz, Benedikt Detz, Hermann Andrews, Aaron M. Lendl, Bernhard Strasser, Gottfried Nat Commun Article Mid-infrared spectroscopy is a sensitive and selective technique for probing molecules in the gas or liquid phase. Investigating chemical reactions in bio-medical applications such as drug production is recently gaining particular interest. However, monitoring dynamic processes in liquids is commonly limited to bulky systems and thus requires time-consuming offline analytics. In this work, we show a next-generation, fully-integrated and robust chip-scale sensor for online measurements of molecule dynamics in a liquid solution. Our fingertip-sized device utilizes quantum cascade technology, combining the emitter, sensing section and detector on a single chip. This enables real-time measurements probing only microliter amounts of analyte in an in situ configuration. We demonstrate time-resolved device operation by analyzing temperature-induced conformational changes of the model protein bovine serum albumin in heavy water. Quantitative measurements reveal excellent performance characteristics in terms of sensor linearity, wide coverage of concentrations, extending from 0.075 mg ml(−1) to 92 mg ml(−1) and a 55-times higher absorbance than state-of-the-art bulky and offline reference systems. Nature Publishing Group UK 2022-08-13 /pmc/articles/PMC9376098/ /pubmed/35963870 http://dx.doi.org/10.1038/s41467-022-32417-7 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Hinkov, Borislav
Pilat, Florian
Lux, Laurin
Souza, Patricia L.
David, Mauro
Schwaighofer, Andreas
Ristanić, Daniela
Schwarz, Benedikt
Detz, Hermann
Andrews, Aaron M.
Lendl, Bernhard
Strasser, Gottfried
A mid-infrared lab-on-a-chip for dynamic reaction monitoring
title A mid-infrared lab-on-a-chip for dynamic reaction monitoring
title_full A mid-infrared lab-on-a-chip for dynamic reaction monitoring
title_fullStr A mid-infrared lab-on-a-chip for dynamic reaction monitoring
title_full_unstemmed A mid-infrared lab-on-a-chip for dynamic reaction monitoring
title_short A mid-infrared lab-on-a-chip for dynamic reaction monitoring
title_sort mid-infrared lab-on-a-chip for dynamic reaction monitoring
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9376098/
https://www.ncbi.nlm.nih.gov/pubmed/35963870
http://dx.doi.org/10.1038/s41467-022-32417-7
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