Cargando…

Erythrocyte sedimentation rate in canine leishmaniosis diagnosis: A new resource

This study aims to evaluate the erythrocyte sedimentation rate (ESR) in Leishmania infantum-seropositive dogs compared with healthy dogs and to assess the existence of a correlation between ESR and clinical form of Canine leishmaniosis (CanL) as well as acute phase proteins (APPs). From October 2021...

Descripción completa

Detalles Bibliográficos
Autores principales: Cavalera, Maria Alfonsa, Gernone, Floriana, Uva, Annamaria, Donghia, Rossella, Carelli, Grazia, Iatta, Roberta, Zatelli, Andrea
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9376380/
https://www.ncbi.nlm.nih.gov/pubmed/35978706
http://dx.doi.org/10.3389/fvets.2022.949372
_version_ 1784768156082896896
author Cavalera, Maria Alfonsa
Gernone, Floriana
Uva, Annamaria
Donghia, Rossella
Carelli, Grazia
Iatta, Roberta
Zatelli, Andrea
author_facet Cavalera, Maria Alfonsa
Gernone, Floriana
Uva, Annamaria
Donghia, Rossella
Carelli, Grazia
Iatta, Roberta
Zatelli, Andrea
author_sort Cavalera, Maria Alfonsa
collection PubMed
description This study aims to evaluate the erythrocyte sedimentation rate (ESR) in Leishmania infantum-seropositive dogs compared with healthy dogs and to assess the existence of a correlation between ESR and clinical form of Canine leishmaniosis (CanL) as well as acute phase proteins (APPs). From October 2021 to January 2022, dogs were recruited in this study if L. infantum-seropositive by enzyme-linked immunoassay and classified as exposed or affected by a CanL active form based on physical examination, clinical score, and laboratory results [i.e., complete blood count, biochemical panel such as C-reactive protein (CRP) and serum ferritin, serum protein electrophoresis, and fibrinogen concentration measurement]. To evaluate the ESR of the dogs, a point-of-care device was used with a reference interval of 0–10 mm/h. Moreover, the ESR evaluation has been also performed in clinically healthy dogs, as control group. Thirty-six L. infantum-seropositive dogs [i.e., exposed (n = 10) and affected by CanL active form (n = 26)] were included in the study. Twenty-two healthy dogs were also enrolled. The mean value of ESR in dogs affected by a CanL active form was significantly higher than in exposed and healthy dogs (p < 0.0001). The ESR level was increased in 92% of dogs with CanL active form while positive APPs such as CRP, fibrinogen, and serum ferritin were increased only in 46, 48, and 58% of the animals, respectively. In exposed dogs, the ESR level was increased in 40% of cases. In dogs with active form, a significant positive correlation between ESR and total proteins, globulins, CRP, and fibrinogen, as well as a significant negative correlation between ESR and hematocrit, hemoglobin, and albumin/globulin ratio were detected. This study provides for the first-time data on ESR in L. infantum-seropositive dogs demonstrating dogs affected by a CanL active form have the highest ESR level and the majority of these dogs presented an increased ESR compared with exposed and healthy dogs. The evaluation of ESR by a point-of-care device proved to be a simple, inexpensive, and ready-to-use benchtop tool and ESR can be considered a helpful and timely inflammatory biomarker for the diagnosis of a CanL active form.
format Online
Article
Text
id pubmed-9376380
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-93763802022-08-16 Erythrocyte sedimentation rate in canine leishmaniosis diagnosis: A new resource Cavalera, Maria Alfonsa Gernone, Floriana Uva, Annamaria Donghia, Rossella Carelli, Grazia Iatta, Roberta Zatelli, Andrea Front Vet Sci Veterinary Science This study aims to evaluate the erythrocyte sedimentation rate (ESR) in Leishmania infantum-seropositive dogs compared with healthy dogs and to assess the existence of a correlation between ESR and clinical form of Canine leishmaniosis (CanL) as well as acute phase proteins (APPs). From October 2021 to January 2022, dogs were recruited in this study if L. infantum-seropositive by enzyme-linked immunoassay and classified as exposed or affected by a CanL active form based on physical examination, clinical score, and laboratory results [i.e., complete blood count, biochemical panel such as C-reactive protein (CRP) and serum ferritin, serum protein electrophoresis, and fibrinogen concentration measurement]. To evaluate the ESR of the dogs, a point-of-care device was used with a reference interval of 0–10 mm/h. Moreover, the ESR evaluation has been also performed in clinically healthy dogs, as control group. Thirty-six L. infantum-seropositive dogs [i.e., exposed (n = 10) and affected by CanL active form (n = 26)] were included in the study. Twenty-two healthy dogs were also enrolled. The mean value of ESR in dogs affected by a CanL active form was significantly higher than in exposed and healthy dogs (p < 0.0001). The ESR level was increased in 92% of dogs with CanL active form while positive APPs such as CRP, fibrinogen, and serum ferritin were increased only in 46, 48, and 58% of the animals, respectively. In exposed dogs, the ESR level was increased in 40% of cases. In dogs with active form, a significant positive correlation between ESR and total proteins, globulins, CRP, and fibrinogen, as well as a significant negative correlation between ESR and hematocrit, hemoglobin, and albumin/globulin ratio were detected. This study provides for the first-time data on ESR in L. infantum-seropositive dogs demonstrating dogs affected by a CanL active form have the highest ESR level and the majority of these dogs presented an increased ESR compared with exposed and healthy dogs. The evaluation of ESR by a point-of-care device proved to be a simple, inexpensive, and ready-to-use benchtop tool and ESR can be considered a helpful and timely inflammatory biomarker for the diagnosis of a CanL active form. Frontiers Media S.A. 2022-08-01 /pmc/articles/PMC9376380/ /pubmed/35978706 http://dx.doi.org/10.3389/fvets.2022.949372 Text en Copyright © 2022 Cavalera, Gernone, Uva, Donghia, Carelli, Iatta and Zatelli. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Cavalera, Maria Alfonsa
Gernone, Floriana
Uva, Annamaria
Donghia, Rossella
Carelli, Grazia
Iatta, Roberta
Zatelli, Andrea
Erythrocyte sedimentation rate in canine leishmaniosis diagnosis: A new resource
title Erythrocyte sedimentation rate in canine leishmaniosis diagnosis: A new resource
title_full Erythrocyte sedimentation rate in canine leishmaniosis diagnosis: A new resource
title_fullStr Erythrocyte sedimentation rate in canine leishmaniosis diagnosis: A new resource
title_full_unstemmed Erythrocyte sedimentation rate in canine leishmaniosis diagnosis: A new resource
title_short Erythrocyte sedimentation rate in canine leishmaniosis diagnosis: A new resource
title_sort erythrocyte sedimentation rate in canine leishmaniosis diagnosis: a new resource
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9376380/
https://www.ncbi.nlm.nih.gov/pubmed/35978706
http://dx.doi.org/10.3389/fvets.2022.949372
work_keys_str_mv AT cavaleramariaalfonsa erythrocytesedimentationrateincanineleishmaniosisdiagnosisanewresource
AT gernonefloriana erythrocytesedimentationrateincanineleishmaniosisdiagnosisanewresource
AT uvaannamaria erythrocytesedimentationrateincanineleishmaniosisdiagnosisanewresource
AT donghiarossella erythrocytesedimentationrateincanineleishmaniosisdiagnosisanewresource
AT carelligrazia erythrocytesedimentationrateincanineleishmaniosisdiagnosisanewresource
AT iattaroberta erythrocytesedimentationrateincanineleishmaniosisdiagnosisanewresource
AT zatelliandrea erythrocytesedimentationrateincanineleishmaniosisdiagnosisanewresource