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Structure and Interaction of Ceramide-Containing Liposomes with Gold Nanoparticles as Characterized by SERS and Cryo-EM

[Image: see text] Due to the great potential of surface-enhanced Raman scattering (SERS) as local vibrational probe of lipid–nanostructure interaction in lipid bilayers, it is important to characterize these interactions in detail. The interpretation of SERS data of lipids in living cells requires a...

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Autores principales: Feng, Yiqing, Kochovski, Zdravko, Arenz, Christoph, Lu, Yan, Kneipp, Janina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9377338/
https://www.ncbi.nlm.nih.gov/pubmed/35983312
http://dx.doi.org/10.1021/acs.jpcc.2c01930
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author Feng, Yiqing
Kochovski, Zdravko
Arenz, Christoph
Lu, Yan
Kneipp, Janina
author_facet Feng, Yiqing
Kochovski, Zdravko
Arenz, Christoph
Lu, Yan
Kneipp, Janina
author_sort Feng, Yiqing
collection PubMed
description [Image: see text] Due to the great potential of surface-enhanced Raman scattering (SERS) as local vibrational probe of lipid–nanostructure interaction in lipid bilayers, it is important to characterize these interactions in detail. The interpretation of SERS data of lipids in living cells requires an understanding of how the molecules interact with gold nanostructures and how intermolecular interactions influence the proximity and contact between lipids and nanoparticles. Ceramide, a sphingolipid that acts as important structural component and regulator of biological function, therefore of interest to probing, lacks a phosphocholine head group that is common to many lipids used in liposome models. SERS spectra of liposomes of a mixture of ceramide, phosphatidic acid, and phosphatidylcholine, as well as of pure ceramide and of the phospholipid mixture are reported. Distinct groups of SERS spectra represent varied contributions of the choline, sphingosine, and phosphate head groups and the structures of the acyl chains. Spectral bands related to the state of order of the membrane and moreover to the amide function of the sphingosine head groups indicate that the gold nanoparticles interact with molecules involved in different intermolecular relations. While cryogenic electron microscopy shows the formation of bilayer liposomes in all preparations, pure ceramide was found to also form supramolecular, concentric stacked and densely packed lamellar, nonliposomal structures. That the formation of such supramolecular assemblies supports the intermolecular interactions of ceramide is indicated by the SERS data. The unique spectral features that are assigned to the ceramide-containing lipid model systems here enable an identification of these molecules in biological systems and allow us to obtain information on their structure and interaction by SERS.
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spelling pubmed-93773382023-07-27 Structure and Interaction of Ceramide-Containing Liposomes with Gold Nanoparticles as Characterized by SERS and Cryo-EM Feng, Yiqing Kochovski, Zdravko Arenz, Christoph Lu, Yan Kneipp, Janina J Phys Chem C Nanomater Interfaces [Image: see text] Due to the great potential of surface-enhanced Raman scattering (SERS) as local vibrational probe of lipid–nanostructure interaction in lipid bilayers, it is important to characterize these interactions in detail. The interpretation of SERS data of lipids in living cells requires an understanding of how the molecules interact with gold nanostructures and how intermolecular interactions influence the proximity and contact between lipids and nanoparticles. Ceramide, a sphingolipid that acts as important structural component and regulator of biological function, therefore of interest to probing, lacks a phosphocholine head group that is common to many lipids used in liposome models. SERS spectra of liposomes of a mixture of ceramide, phosphatidic acid, and phosphatidylcholine, as well as of pure ceramide and of the phospholipid mixture are reported. Distinct groups of SERS spectra represent varied contributions of the choline, sphingosine, and phosphate head groups and the structures of the acyl chains. Spectral bands related to the state of order of the membrane and moreover to the amide function of the sphingosine head groups indicate that the gold nanoparticles interact with molecules involved in different intermolecular relations. While cryogenic electron microscopy shows the formation of bilayer liposomes in all preparations, pure ceramide was found to also form supramolecular, concentric stacked and densely packed lamellar, nonliposomal structures. That the formation of such supramolecular assemblies supports the intermolecular interactions of ceramide is indicated by the SERS data. The unique spectral features that are assigned to the ceramide-containing lipid model systems here enable an identification of these molecules in biological systems and allow us to obtain information on their structure and interaction by SERS. American Chemical Society 2022-07-27 2022-08-11 /pmc/articles/PMC9377338/ /pubmed/35983312 http://dx.doi.org/10.1021/acs.jpcc.2c01930 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Feng, Yiqing
Kochovski, Zdravko
Arenz, Christoph
Lu, Yan
Kneipp, Janina
Structure and Interaction of Ceramide-Containing Liposomes with Gold Nanoparticles as Characterized by SERS and Cryo-EM
title Structure and Interaction of Ceramide-Containing Liposomes with Gold Nanoparticles as Characterized by SERS and Cryo-EM
title_full Structure and Interaction of Ceramide-Containing Liposomes with Gold Nanoparticles as Characterized by SERS and Cryo-EM
title_fullStr Structure and Interaction of Ceramide-Containing Liposomes with Gold Nanoparticles as Characterized by SERS and Cryo-EM
title_full_unstemmed Structure and Interaction of Ceramide-Containing Liposomes with Gold Nanoparticles as Characterized by SERS and Cryo-EM
title_short Structure and Interaction of Ceramide-Containing Liposomes with Gold Nanoparticles as Characterized by SERS and Cryo-EM
title_sort structure and interaction of ceramide-containing liposomes with gold nanoparticles as characterized by sers and cryo-em
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9377338/
https://www.ncbi.nlm.nih.gov/pubmed/35983312
http://dx.doi.org/10.1021/acs.jpcc.2c01930
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