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Worenine Prevents Solar Ultraviolet–Induced Sunburn by Inhibiting JNK2

Excessive solar ultraviolet (SUV) radiation often causes dermatitis, photoaging, and even skin cancer. In the pathological processes of SUV-induced sunburn, JNK is activated by phosphorylation, and it in turn phosphorylates its downstream transcription factors, such as ATF2 and c-jun. The transcript...

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Autores principales: Xiao, Juanjuan, Lu, Hui, Ma, Tengfei, Ni, Xiaofang, Chang, Teding, Liu, Man, Li, Nijie, Lu, Peijiang, Ke, Changshu, Tian, Qin, Zou, Ling, Wang, Fei, Wang, Wei, Zhang, Lu, Yuan, Ping, Liu, Lin, Zhang, Jianmin, Shi, Fei, Duan, Qiuhong, Zhu, Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9377457/
https://www.ncbi.nlm.nih.gov/pubmed/35979232
http://dx.doi.org/10.3389/fphar.2022.881042
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author Xiao, Juanjuan
Lu, Hui
Ma, Tengfei
Ni, Xiaofang
Chang, Teding
Liu, Man
Li, Nijie
Lu, Peijiang
Ke, Changshu
Tian, Qin
Zou, Ling
Wang, Fei
Wang, Wei
Zhang, Lu
Yuan, Ping
Liu, Lin
Zhang, Jianmin
Shi, Fei
Duan, Qiuhong
Zhu, Feng
author_facet Xiao, Juanjuan
Lu, Hui
Ma, Tengfei
Ni, Xiaofang
Chang, Teding
Liu, Man
Li, Nijie
Lu, Peijiang
Ke, Changshu
Tian, Qin
Zou, Ling
Wang, Fei
Wang, Wei
Zhang, Lu
Yuan, Ping
Liu, Lin
Zhang, Jianmin
Shi, Fei
Duan, Qiuhong
Zhu, Feng
author_sort Xiao, Juanjuan
collection PubMed
description Excessive solar ultraviolet (SUV) radiation often causes dermatitis, photoaging, and even skin cancer. In the pathological processes of SUV-induced sunburn, JNK is activated by phosphorylation, and it in turn phosphorylates its downstream transcription factors, such as ATF2 and c-jun. The transcription factors further regulate the expression of pro-inflammatory genes, such as IL-6 and TNF-α, which ultimately leads to dermatitis. Therefore, inhibiting JNK may be a strategy to prevent dermatitis. In this study, we screened for worenine as a potential drug candidate for inhibiting sunburn. We determined that worenine inhibited the JNK-ATF2/c-jun signaling pathway and the secretion of IL-6 and TNF-α in cell culture and in vivo, confirming the role of worenine in inhibiting sunburn. Furthermore, we determined that worenine bound and inhibited JNK2 activity in vitro through the MST, kinase, and in vitro kinase assays. Therefore, worenine might be a promising drug candidate for the prevention and treatment of SUV-induced sunburn.
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spelling pubmed-93774572022-08-16 Worenine Prevents Solar Ultraviolet–Induced Sunburn by Inhibiting JNK2 Xiao, Juanjuan Lu, Hui Ma, Tengfei Ni, Xiaofang Chang, Teding Liu, Man Li, Nijie Lu, Peijiang Ke, Changshu Tian, Qin Zou, Ling Wang, Fei Wang, Wei Zhang, Lu Yuan, Ping Liu, Lin Zhang, Jianmin Shi, Fei Duan, Qiuhong Zhu, Feng Front Pharmacol Pharmacology Excessive solar ultraviolet (SUV) radiation often causes dermatitis, photoaging, and even skin cancer. In the pathological processes of SUV-induced sunburn, JNK is activated by phosphorylation, and it in turn phosphorylates its downstream transcription factors, such as ATF2 and c-jun. The transcription factors further regulate the expression of pro-inflammatory genes, such as IL-6 and TNF-α, which ultimately leads to dermatitis. Therefore, inhibiting JNK may be a strategy to prevent dermatitis. In this study, we screened for worenine as a potential drug candidate for inhibiting sunburn. We determined that worenine inhibited the JNK-ATF2/c-jun signaling pathway and the secretion of IL-6 and TNF-α in cell culture and in vivo, confirming the role of worenine in inhibiting sunburn. Furthermore, we determined that worenine bound and inhibited JNK2 activity in vitro through the MST, kinase, and in vitro kinase assays. Therefore, worenine might be a promising drug candidate for the prevention and treatment of SUV-induced sunburn. Frontiers Media S.A. 2022-07-22 /pmc/articles/PMC9377457/ /pubmed/35979232 http://dx.doi.org/10.3389/fphar.2022.881042 Text en Copyright © 2022 Xiao, Lu, Ma, Ni, Chang, Liu, Li, Lu, Ke, Tian, Zou, Wang, Wang, Zhang, Yuan, Liu, Zhang, Shi, Duan and Zhu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Xiao, Juanjuan
Lu, Hui
Ma, Tengfei
Ni, Xiaofang
Chang, Teding
Liu, Man
Li, Nijie
Lu, Peijiang
Ke, Changshu
Tian, Qin
Zou, Ling
Wang, Fei
Wang, Wei
Zhang, Lu
Yuan, Ping
Liu, Lin
Zhang, Jianmin
Shi, Fei
Duan, Qiuhong
Zhu, Feng
Worenine Prevents Solar Ultraviolet–Induced Sunburn by Inhibiting JNK2
title Worenine Prevents Solar Ultraviolet–Induced Sunburn by Inhibiting JNK2
title_full Worenine Prevents Solar Ultraviolet–Induced Sunburn by Inhibiting JNK2
title_fullStr Worenine Prevents Solar Ultraviolet–Induced Sunburn by Inhibiting JNK2
title_full_unstemmed Worenine Prevents Solar Ultraviolet–Induced Sunburn by Inhibiting JNK2
title_short Worenine Prevents Solar Ultraviolet–Induced Sunburn by Inhibiting JNK2
title_sort worenine prevents solar ultraviolet–induced sunburn by inhibiting jnk2
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9377457/
https://www.ncbi.nlm.nih.gov/pubmed/35979232
http://dx.doi.org/10.3389/fphar.2022.881042
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