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Endophytic fungus Serendipita indica accelerates ascorbate-glutathione cycle of white clover in response to water stress

Ascorbate-glutathione cycle is an important pathway for plants to scavenge reactive oxygen species (ROS) under environmental stress conditions. The objective of this study was to investigate the effects of the endophytic fungus Serendipita indica on biomass, chlorophyll concent, ROS levels, antioxid...

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Autores principales: Rong, Zi-Yi, Jiang, Dao-Ju, Cao, Jin-Li, Hashem, Abeer, Abd_Allah, Elsayed Fathi, Alsayed, Mashail Fahad, Harsonowati, Wiwiek, Wu, Qiang-Sheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9377509/
https://www.ncbi.nlm.nih.gov/pubmed/35979492
http://dx.doi.org/10.3389/fmicb.2022.967851
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author Rong, Zi-Yi
Jiang, Dao-Ju
Cao, Jin-Li
Hashem, Abeer
Abd_Allah, Elsayed Fathi
Alsayed, Mashail Fahad
Harsonowati, Wiwiek
Wu, Qiang-Sheng
author_facet Rong, Zi-Yi
Jiang, Dao-Ju
Cao, Jin-Li
Hashem, Abeer
Abd_Allah, Elsayed Fathi
Alsayed, Mashail Fahad
Harsonowati, Wiwiek
Wu, Qiang-Sheng
author_sort Rong, Zi-Yi
collection PubMed
description Ascorbate-glutathione cycle is an important pathway for plants to scavenge reactive oxygen species (ROS) under environmental stress conditions. The objective of this study was to investigate the effects of the endophytic fungus Serendipita indica on biomass, chlorophyll concent, ROS levels, antioxidant enzyme activities, and ascorbate-glutathione cycle in white clover under ample water and water stress conditions. The results showed that 46 days of soil water stress distinctly promoted root colonization by S. indica. Under water stress, S. indica inoculation significantly promoted shoot and root biomass, total chlorophyll content, and activities of superoxide dismutases (SOD; e.g., Fe-SOD and Cu/Zn-SOD) and peroxidase in roots, coupled with a decrease in malondialdehyde content in roots. In the ascorbate-glutathione cycle of roots, S. indica also significantly increased the activity of ascorbate peroxidase and glutathione reductase activities in water-stressed white clover, along with the increase in reduced ascorbic acid and reduced/oxidized glutathione contents, thus accelerating the ascorbate-glutathione cycle in inoculated plants to scavenge more ROS (e.g., hydrogen peroxide). As a result, S. indica enhanced the tolerance of white clover in response to water stress by enhancing antioxidant enzyme activities and accelerating the ascorbate-glutathione cycle.
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spelling pubmed-93775092022-08-16 Endophytic fungus Serendipita indica accelerates ascorbate-glutathione cycle of white clover in response to water stress Rong, Zi-Yi Jiang, Dao-Ju Cao, Jin-Li Hashem, Abeer Abd_Allah, Elsayed Fathi Alsayed, Mashail Fahad Harsonowati, Wiwiek Wu, Qiang-Sheng Front Microbiol Microbiology Ascorbate-glutathione cycle is an important pathway for plants to scavenge reactive oxygen species (ROS) under environmental stress conditions. The objective of this study was to investigate the effects of the endophytic fungus Serendipita indica on biomass, chlorophyll concent, ROS levels, antioxidant enzyme activities, and ascorbate-glutathione cycle in white clover under ample water and water stress conditions. The results showed that 46 days of soil water stress distinctly promoted root colonization by S. indica. Under water stress, S. indica inoculation significantly promoted shoot and root biomass, total chlorophyll content, and activities of superoxide dismutases (SOD; e.g., Fe-SOD and Cu/Zn-SOD) and peroxidase in roots, coupled with a decrease in malondialdehyde content in roots. In the ascorbate-glutathione cycle of roots, S. indica also significantly increased the activity of ascorbate peroxidase and glutathione reductase activities in water-stressed white clover, along with the increase in reduced ascorbic acid and reduced/oxidized glutathione contents, thus accelerating the ascorbate-glutathione cycle in inoculated plants to scavenge more ROS (e.g., hydrogen peroxide). As a result, S. indica enhanced the tolerance of white clover in response to water stress by enhancing antioxidant enzyme activities and accelerating the ascorbate-glutathione cycle. Frontiers Media S.A. 2022-08-01 /pmc/articles/PMC9377509/ /pubmed/35979492 http://dx.doi.org/10.3389/fmicb.2022.967851 Text en Copyright © 2022 Rong, Jiang, Cao, Hashem, Abd_Allah, Alsayed, Harsonowati and Wu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Rong, Zi-Yi
Jiang, Dao-Ju
Cao, Jin-Li
Hashem, Abeer
Abd_Allah, Elsayed Fathi
Alsayed, Mashail Fahad
Harsonowati, Wiwiek
Wu, Qiang-Sheng
Endophytic fungus Serendipita indica accelerates ascorbate-glutathione cycle of white clover in response to water stress
title Endophytic fungus Serendipita indica accelerates ascorbate-glutathione cycle of white clover in response to water stress
title_full Endophytic fungus Serendipita indica accelerates ascorbate-glutathione cycle of white clover in response to water stress
title_fullStr Endophytic fungus Serendipita indica accelerates ascorbate-glutathione cycle of white clover in response to water stress
title_full_unstemmed Endophytic fungus Serendipita indica accelerates ascorbate-glutathione cycle of white clover in response to water stress
title_short Endophytic fungus Serendipita indica accelerates ascorbate-glutathione cycle of white clover in response to water stress
title_sort endophytic fungus serendipita indica accelerates ascorbate-glutathione cycle of white clover in response to water stress
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9377509/
https://www.ncbi.nlm.nih.gov/pubmed/35979492
http://dx.doi.org/10.3389/fmicb.2022.967851
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