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Screening and evaluation of lactic acid bacteria with probiotic potential from local Holstein raw milk

There are massive bacteria in the raw milk, especially the lactic acid bacteria (LABs), which have been considered probiotics in humans and animals for a long time. Novel probiotics are still urgently needed because of the rapid development of the probiotic industry. To obtain new LABs with high pro...

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Autores principales: Zhang, Wenqing, Lai, Shiji, Zhou, Ziyao, Yang, Jinpeng, Liu, Haifeng, Zhong, Zhijun, Fu, Hualin, Ren, Zhihua, Shen, Liuhong, Cao, Suizhong, Deng, Lei, Peng, Guangneng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9377552/
https://www.ncbi.nlm.nih.gov/pubmed/35979483
http://dx.doi.org/10.3389/fmicb.2022.918774
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author Zhang, Wenqing
Lai, Shiji
Zhou, Ziyao
Yang, Jinpeng
Liu, Haifeng
Zhong, Zhijun
Fu, Hualin
Ren, Zhihua
Shen, Liuhong
Cao, Suizhong
Deng, Lei
Peng, Guangneng
author_facet Zhang, Wenqing
Lai, Shiji
Zhou, Ziyao
Yang, Jinpeng
Liu, Haifeng
Zhong, Zhijun
Fu, Hualin
Ren, Zhihua
Shen, Liuhong
Cao, Suizhong
Deng, Lei
Peng, Guangneng
author_sort Zhang, Wenqing
collection PubMed
description There are massive bacteria in the raw milk, especially the lactic acid bacteria (LABs), which have been considered probiotics in humans and animals for a long time. Novel probiotics are still urgently needed because of the rapid development of the probiotic industry. To obtain new LABs with high probiotic potential, we obtained 26 LAB isolates, named L1 ~ L26, from local Holstein raw milk collected from a farm whose milk had never been used for LAB isolation. We identified them at the species level by biochemical and 16S rDNA sequencing methods. Their antagonistic activities against four target pathogens (Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa PAO1, and Salmonella enterica H9812), co-aggregative ability with these target pathogens, survivability in the simulated gastrointestinal tract conditions and phenol, auto-aggregation and hydrophobicity, hemolytic activity, and antibiotic susceptibility, were evaluated in vitro. Five Lactiplantibacillus plantarum isolates (L5, L14, L17, L19, and L20) showed more promising probiotic potential than others. Specifically, these five isolates conglutinated with and inhibited all the target pathogens, and survived in the simulated gastric juice (92.55 ~ 99.69%), intestinal juice (76.18 ~ 83.39%), and 0.4% phenol (76.95 ~ 88.91%); possessed considerable auto-aggregation (83.91 ~ 90.33% at 24 h) and hydrophobicity (79.32 ~ 92.70%); and were non-hemolytic, sensitive to kinds of common antimicrobials. Our findings demonstrated that these five isolates could be preliminarily determined as probiotic candidates because they have better probiotic potential than those previously reported. Again, this study highlighted the potential of raw milk for probiotic isolating and screening and provided the probiotic industry with five new LAB candidates.
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spelling pubmed-93775522022-08-16 Screening and evaluation of lactic acid bacteria with probiotic potential from local Holstein raw milk Zhang, Wenqing Lai, Shiji Zhou, Ziyao Yang, Jinpeng Liu, Haifeng Zhong, Zhijun Fu, Hualin Ren, Zhihua Shen, Liuhong Cao, Suizhong Deng, Lei Peng, Guangneng Front Microbiol Microbiology There are massive bacteria in the raw milk, especially the lactic acid bacteria (LABs), which have been considered probiotics in humans and animals for a long time. Novel probiotics are still urgently needed because of the rapid development of the probiotic industry. To obtain new LABs with high probiotic potential, we obtained 26 LAB isolates, named L1 ~ L26, from local Holstein raw milk collected from a farm whose milk had never been used for LAB isolation. We identified them at the species level by biochemical and 16S rDNA sequencing methods. Their antagonistic activities against four target pathogens (Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa PAO1, and Salmonella enterica H9812), co-aggregative ability with these target pathogens, survivability in the simulated gastrointestinal tract conditions and phenol, auto-aggregation and hydrophobicity, hemolytic activity, and antibiotic susceptibility, were evaluated in vitro. Five Lactiplantibacillus plantarum isolates (L5, L14, L17, L19, and L20) showed more promising probiotic potential than others. Specifically, these five isolates conglutinated with and inhibited all the target pathogens, and survived in the simulated gastric juice (92.55 ~ 99.69%), intestinal juice (76.18 ~ 83.39%), and 0.4% phenol (76.95 ~ 88.91%); possessed considerable auto-aggregation (83.91 ~ 90.33% at 24 h) and hydrophobicity (79.32 ~ 92.70%); and were non-hemolytic, sensitive to kinds of common antimicrobials. Our findings demonstrated that these five isolates could be preliminarily determined as probiotic candidates because they have better probiotic potential than those previously reported. Again, this study highlighted the potential of raw milk for probiotic isolating and screening and provided the probiotic industry with five new LAB candidates. Frontiers Media S.A. 2022-08-01 /pmc/articles/PMC9377552/ /pubmed/35979483 http://dx.doi.org/10.3389/fmicb.2022.918774 Text en Copyright © 2022 Zhang, Yang, Zhou, Deng, Lai, Liu, Zhong, Fu, Ren, Shen, Cao and Peng. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Zhang, Wenqing
Lai, Shiji
Zhou, Ziyao
Yang, Jinpeng
Liu, Haifeng
Zhong, Zhijun
Fu, Hualin
Ren, Zhihua
Shen, Liuhong
Cao, Suizhong
Deng, Lei
Peng, Guangneng
Screening and evaluation of lactic acid bacteria with probiotic potential from local Holstein raw milk
title Screening and evaluation of lactic acid bacteria with probiotic potential from local Holstein raw milk
title_full Screening and evaluation of lactic acid bacteria with probiotic potential from local Holstein raw milk
title_fullStr Screening and evaluation of lactic acid bacteria with probiotic potential from local Holstein raw milk
title_full_unstemmed Screening and evaluation of lactic acid bacteria with probiotic potential from local Holstein raw milk
title_short Screening and evaluation of lactic acid bacteria with probiotic potential from local Holstein raw milk
title_sort screening and evaluation of lactic acid bacteria with probiotic potential from local holstein raw milk
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9377552/
https://www.ncbi.nlm.nih.gov/pubmed/35979483
http://dx.doi.org/10.3389/fmicb.2022.918774
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