Cargando…
Screening and evaluation of lactic acid bacteria with probiotic potential from local Holstein raw milk
There are massive bacteria in the raw milk, especially the lactic acid bacteria (LABs), which have been considered probiotics in humans and animals for a long time. Novel probiotics are still urgently needed because of the rapid development of the probiotic industry. To obtain new LABs with high pro...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9377552/ https://www.ncbi.nlm.nih.gov/pubmed/35979483 http://dx.doi.org/10.3389/fmicb.2022.918774 |
_version_ | 1784768361949822976 |
---|---|
author | Zhang, Wenqing Lai, Shiji Zhou, Ziyao Yang, Jinpeng Liu, Haifeng Zhong, Zhijun Fu, Hualin Ren, Zhihua Shen, Liuhong Cao, Suizhong Deng, Lei Peng, Guangneng |
author_facet | Zhang, Wenqing Lai, Shiji Zhou, Ziyao Yang, Jinpeng Liu, Haifeng Zhong, Zhijun Fu, Hualin Ren, Zhihua Shen, Liuhong Cao, Suizhong Deng, Lei Peng, Guangneng |
author_sort | Zhang, Wenqing |
collection | PubMed |
description | There are massive bacteria in the raw milk, especially the lactic acid bacteria (LABs), which have been considered probiotics in humans and animals for a long time. Novel probiotics are still urgently needed because of the rapid development of the probiotic industry. To obtain new LABs with high probiotic potential, we obtained 26 LAB isolates, named L1 ~ L26, from local Holstein raw milk collected from a farm whose milk had never been used for LAB isolation. We identified them at the species level by biochemical and 16S rDNA sequencing methods. Their antagonistic activities against four target pathogens (Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa PAO1, and Salmonella enterica H9812), co-aggregative ability with these target pathogens, survivability in the simulated gastrointestinal tract conditions and phenol, auto-aggregation and hydrophobicity, hemolytic activity, and antibiotic susceptibility, were evaluated in vitro. Five Lactiplantibacillus plantarum isolates (L5, L14, L17, L19, and L20) showed more promising probiotic potential than others. Specifically, these five isolates conglutinated with and inhibited all the target pathogens, and survived in the simulated gastric juice (92.55 ~ 99.69%), intestinal juice (76.18 ~ 83.39%), and 0.4% phenol (76.95 ~ 88.91%); possessed considerable auto-aggregation (83.91 ~ 90.33% at 24 h) and hydrophobicity (79.32 ~ 92.70%); and were non-hemolytic, sensitive to kinds of common antimicrobials. Our findings demonstrated that these five isolates could be preliminarily determined as probiotic candidates because they have better probiotic potential than those previously reported. Again, this study highlighted the potential of raw milk for probiotic isolating and screening and provided the probiotic industry with five new LAB candidates. |
format | Online Article Text |
id | pubmed-9377552 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-93775522022-08-16 Screening and evaluation of lactic acid bacteria with probiotic potential from local Holstein raw milk Zhang, Wenqing Lai, Shiji Zhou, Ziyao Yang, Jinpeng Liu, Haifeng Zhong, Zhijun Fu, Hualin Ren, Zhihua Shen, Liuhong Cao, Suizhong Deng, Lei Peng, Guangneng Front Microbiol Microbiology There are massive bacteria in the raw milk, especially the lactic acid bacteria (LABs), which have been considered probiotics in humans and animals for a long time. Novel probiotics are still urgently needed because of the rapid development of the probiotic industry. To obtain new LABs with high probiotic potential, we obtained 26 LAB isolates, named L1 ~ L26, from local Holstein raw milk collected from a farm whose milk had never been used for LAB isolation. We identified them at the species level by biochemical and 16S rDNA sequencing methods. Their antagonistic activities against four target pathogens (Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa PAO1, and Salmonella enterica H9812), co-aggregative ability with these target pathogens, survivability in the simulated gastrointestinal tract conditions and phenol, auto-aggregation and hydrophobicity, hemolytic activity, and antibiotic susceptibility, were evaluated in vitro. Five Lactiplantibacillus plantarum isolates (L5, L14, L17, L19, and L20) showed more promising probiotic potential than others. Specifically, these five isolates conglutinated with and inhibited all the target pathogens, and survived in the simulated gastric juice (92.55 ~ 99.69%), intestinal juice (76.18 ~ 83.39%), and 0.4% phenol (76.95 ~ 88.91%); possessed considerable auto-aggregation (83.91 ~ 90.33% at 24 h) and hydrophobicity (79.32 ~ 92.70%); and were non-hemolytic, sensitive to kinds of common antimicrobials. Our findings demonstrated that these five isolates could be preliminarily determined as probiotic candidates because they have better probiotic potential than those previously reported. Again, this study highlighted the potential of raw milk for probiotic isolating and screening and provided the probiotic industry with five new LAB candidates. Frontiers Media S.A. 2022-08-01 /pmc/articles/PMC9377552/ /pubmed/35979483 http://dx.doi.org/10.3389/fmicb.2022.918774 Text en Copyright © 2022 Zhang, Yang, Zhou, Deng, Lai, Liu, Zhong, Fu, Ren, Shen, Cao and Peng. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Zhang, Wenqing Lai, Shiji Zhou, Ziyao Yang, Jinpeng Liu, Haifeng Zhong, Zhijun Fu, Hualin Ren, Zhihua Shen, Liuhong Cao, Suizhong Deng, Lei Peng, Guangneng Screening and evaluation of lactic acid bacteria with probiotic potential from local Holstein raw milk |
title | Screening and evaluation of lactic acid bacteria with probiotic potential from local Holstein raw milk |
title_full | Screening and evaluation of lactic acid bacteria with probiotic potential from local Holstein raw milk |
title_fullStr | Screening and evaluation of lactic acid bacteria with probiotic potential from local Holstein raw milk |
title_full_unstemmed | Screening and evaluation of lactic acid bacteria with probiotic potential from local Holstein raw milk |
title_short | Screening and evaluation of lactic acid bacteria with probiotic potential from local Holstein raw milk |
title_sort | screening and evaluation of lactic acid bacteria with probiotic potential from local holstein raw milk |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9377552/ https://www.ncbi.nlm.nih.gov/pubmed/35979483 http://dx.doi.org/10.3389/fmicb.2022.918774 |
work_keys_str_mv | AT zhangwenqing screeningandevaluationoflacticacidbacteriawithprobioticpotentialfromlocalholsteinrawmilk AT laishiji screeningandevaluationoflacticacidbacteriawithprobioticpotentialfromlocalholsteinrawmilk AT zhouziyao screeningandevaluationoflacticacidbacteriawithprobioticpotentialfromlocalholsteinrawmilk AT yangjinpeng screeningandevaluationoflacticacidbacteriawithprobioticpotentialfromlocalholsteinrawmilk AT liuhaifeng screeningandevaluationoflacticacidbacteriawithprobioticpotentialfromlocalholsteinrawmilk AT zhongzhijun screeningandevaluationoflacticacidbacteriawithprobioticpotentialfromlocalholsteinrawmilk AT fuhualin screeningandevaluationoflacticacidbacteriawithprobioticpotentialfromlocalholsteinrawmilk AT renzhihua screeningandevaluationoflacticacidbacteriawithprobioticpotentialfromlocalholsteinrawmilk AT shenliuhong screeningandevaluationoflacticacidbacteriawithprobioticpotentialfromlocalholsteinrawmilk AT caosuizhong screeningandevaluationoflacticacidbacteriawithprobioticpotentialfromlocalholsteinrawmilk AT denglei screeningandevaluationoflacticacidbacteriawithprobioticpotentialfromlocalholsteinrawmilk AT pengguangneng screeningandevaluationoflacticacidbacteriawithprobioticpotentialfromlocalholsteinrawmilk |