Cargando…
Anti-inflammatory effects of neutral lipids, glycolipids, phospholipids from Halocynthia aurantium tunic by suppressing the activation of NF-κB and MAPKs in LPS-stimulated RAW264.7 macrophages
Halocynthia aurantium is a marine organism that has been considered a promising source for bio-functional materials. Total lipids were extracted from H. aurantium tunic, and then they were separated into neutral lipids, glycolipids, and phospholipids. In the present study, fatty acid profiles of thr...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9377571/ https://www.ncbi.nlm.nih.gov/pubmed/35969529 http://dx.doi.org/10.1371/journal.pone.0270794 |
_version_ | 1784768366068629504 |
---|---|
author | Jang, A-yeong Rod-in, Weerawan Monmai, Chaiwat Choi, Gyoung Su Park, Woo Jung |
author_facet | Jang, A-yeong Rod-in, Weerawan Monmai, Chaiwat Choi, Gyoung Su Park, Woo Jung |
author_sort | Jang, A-yeong |
collection | PubMed |
description | Halocynthia aurantium is a marine organism that has been considered a promising source for bio-functional materials. Total lipids were extracted from H. aurantium tunic, and then they were separated into neutral lipids, glycolipids, and phospholipids. In the present study, fatty acid profiles of three lipids and their anti-inflammatory effects in RAW264.7 cells were investigated. Among the lipid classes, phospholipids showed the diversity of fatty acid constituents, compared with the glycolipids and neutral lipids. Three lipids contain different contents of fatty acids depending on the kinds of lipids. The most contents were saturated fatty acids (SFAs, 53–69% of the fatty acids) and monounsaturated fatty acids (MUFAs, 15–17% of fatty acids) and polyunsaturated fatty acids (PUFAs, 14–32% of fatty acids) are followed. H. aurantium lipids not only dose-dependently inhibited nitric oxide production but also reduced the expression of inflammatory cytokine genes such as TNF-α, IL-1β, and IL-6 in LPS-stimulated macrophages. It was also demonstrated that the expression of COX-2 was dose-dependently suppressed. Moreover, H. aurantium lipids decreased phosphorylation of NF-κB p-65, p38, ERK1/2, and JNK, suggesting that three lipids from H. aurantium tunic provide anti-inflammatory effects through NF-κB and MAPK signaling. These results indicate that H. aurantium is a potential source for anti-inflammation. |
format | Online Article Text |
id | pubmed-9377571 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-93775712022-08-16 Anti-inflammatory effects of neutral lipids, glycolipids, phospholipids from Halocynthia aurantium tunic by suppressing the activation of NF-κB and MAPKs in LPS-stimulated RAW264.7 macrophages Jang, A-yeong Rod-in, Weerawan Monmai, Chaiwat Choi, Gyoung Su Park, Woo Jung PLoS One Research Article Halocynthia aurantium is a marine organism that has been considered a promising source for bio-functional materials. Total lipids were extracted from H. aurantium tunic, and then they were separated into neutral lipids, glycolipids, and phospholipids. In the present study, fatty acid profiles of three lipids and their anti-inflammatory effects in RAW264.7 cells were investigated. Among the lipid classes, phospholipids showed the diversity of fatty acid constituents, compared with the glycolipids and neutral lipids. Three lipids contain different contents of fatty acids depending on the kinds of lipids. The most contents were saturated fatty acids (SFAs, 53–69% of the fatty acids) and monounsaturated fatty acids (MUFAs, 15–17% of fatty acids) and polyunsaturated fatty acids (PUFAs, 14–32% of fatty acids) are followed. H. aurantium lipids not only dose-dependently inhibited nitric oxide production but also reduced the expression of inflammatory cytokine genes such as TNF-α, IL-1β, and IL-6 in LPS-stimulated macrophages. It was also demonstrated that the expression of COX-2 was dose-dependently suppressed. Moreover, H. aurantium lipids decreased phosphorylation of NF-κB p-65, p38, ERK1/2, and JNK, suggesting that three lipids from H. aurantium tunic provide anti-inflammatory effects through NF-κB and MAPK signaling. These results indicate that H. aurantium is a potential source for anti-inflammation. Public Library of Science 2022-08-15 /pmc/articles/PMC9377571/ /pubmed/35969529 http://dx.doi.org/10.1371/journal.pone.0270794 Text en © 2022 Jang et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Jang, A-yeong Rod-in, Weerawan Monmai, Chaiwat Choi, Gyoung Su Park, Woo Jung Anti-inflammatory effects of neutral lipids, glycolipids, phospholipids from Halocynthia aurantium tunic by suppressing the activation of NF-κB and MAPKs in LPS-stimulated RAW264.7 macrophages |
title | Anti-inflammatory effects of neutral lipids, glycolipids, phospholipids from Halocynthia aurantium tunic by suppressing the activation of NF-κB and MAPKs in LPS-stimulated RAW264.7 macrophages |
title_full | Anti-inflammatory effects of neutral lipids, glycolipids, phospholipids from Halocynthia aurantium tunic by suppressing the activation of NF-κB and MAPKs in LPS-stimulated RAW264.7 macrophages |
title_fullStr | Anti-inflammatory effects of neutral lipids, glycolipids, phospholipids from Halocynthia aurantium tunic by suppressing the activation of NF-κB and MAPKs in LPS-stimulated RAW264.7 macrophages |
title_full_unstemmed | Anti-inflammatory effects of neutral lipids, glycolipids, phospholipids from Halocynthia aurantium tunic by suppressing the activation of NF-κB and MAPKs in LPS-stimulated RAW264.7 macrophages |
title_short | Anti-inflammatory effects of neutral lipids, glycolipids, phospholipids from Halocynthia aurantium tunic by suppressing the activation of NF-κB and MAPKs in LPS-stimulated RAW264.7 macrophages |
title_sort | anti-inflammatory effects of neutral lipids, glycolipids, phospholipids from halocynthia aurantium tunic by suppressing the activation of nf-κb and mapks in lps-stimulated raw264.7 macrophages |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9377571/ https://www.ncbi.nlm.nih.gov/pubmed/35969529 http://dx.doi.org/10.1371/journal.pone.0270794 |
work_keys_str_mv | AT jangayeong antiinflammatoryeffectsofneutrallipidsglycolipidsphospholipidsfromhalocynthiaaurantiumtunicbysuppressingtheactivationofnfkbandmapksinlpsstimulatedraw2647macrophages AT rodinweerawan antiinflammatoryeffectsofneutrallipidsglycolipidsphospholipidsfromhalocynthiaaurantiumtunicbysuppressingtheactivationofnfkbandmapksinlpsstimulatedraw2647macrophages AT monmaichaiwat antiinflammatoryeffectsofneutrallipidsglycolipidsphospholipidsfromhalocynthiaaurantiumtunicbysuppressingtheactivationofnfkbandmapksinlpsstimulatedraw2647macrophages AT choigyoungsu antiinflammatoryeffectsofneutrallipidsglycolipidsphospholipidsfromhalocynthiaaurantiumtunicbysuppressingtheactivationofnfkbandmapksinlpsstimulatedraw2647macrophages AT parkwoojung antiinflammatoryeffectsofneutrallipidsglycolipidsphospholipidsfromhalocynthiaaurantiumtunicbysuppressingtheactivationofnfkbandmapksinlpsstimulatedraw2647macrophages |