Cargando…

Anti-inflammatory effects of neutral lipids, glycolipids, phospholipids from Halocynthia aurantium tunic by suppressing the activation of NF-κB and MAPKs in LPS-stimulated RAW264.7 macrophages

Halocynthia aurantium is a marine organism that has been considered a promising source for bio-functional materials. Total lipids were extracted from H. aurantium tunic, and then they were separated into neutral lipids, glycolipids, and phospholipids. In the present study, fatty acid profiles of thr...

Descripción completa

Detalles Bibliográficos
Autores principales: Jang, A-yeong, Rod-in, Weerawan, Monmai, Chaiwat, Choi, Gyoung Su, Park, Woo Jung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9377571/
https://www.ncbi.nlm.nih.gov/pubmed/35969529
http://dx.doi.org/10.1371/journal.pone.0270794
_version_ 1784768366068629504
author Jang, A-yeong
Rod-in, Weerawan
Monmai, Chaiwat
Choi, Gyoung Su
Park, Woo Jung
author_facet Jang, A-yeong
Rod-in, Weerawan
Monmai, Chaiwat
Choi, Gyoung Su
Park, Woo Jung
author_sort Jang, A-yeong
collection PubMed
description Halocynthia aurantium is a marine organism that has been considered a promising source for bio-functional materials. Total lipids were extracted from H. aurantium tunic, and then they were separated into neutral lipids, glycolipids, and phospholipids. In the present study, fatty acid profiles of three lipids and their anti-inflammatory effects in RAW264.7 cells were investigated. Among the lipid classes, phospholipids showed the diversity of fatty acid constituents, compared with the glycolipids and neutral lipids. Three lipids contain different contents of fatty acids depending on the kinds of lipids. The most contents were saturated fatty acids (SFAs, 53–69% of the fatty acids) and monounsaturated fatty acids (MUFAs, 15–17% of fatty acids) and polyunsaturated fatty acids (PUFAs, 14–32% of fatty acids) are followed. H. aurantium lipids not only dose-dependently inhibited nitric oxide production but also reduced the expression of inflammatory cytokine genes such as TNF-α, IL-1β, and IL-6 in LPS-stimulated macrophages. It was also demonstrated that the expression of COX-2 was dose-dependently suppressed. Moreover, H. aurantium lipids decreased phosphorylation of NF-κB p-65, p38, ERK1/2, and JNK, suggesting that three lipids from H. aurantium tunic provide anti-inflammatory effects through NF-κB and MAPK signaling. These results indicate that H. aurantium is a potential source for anti-inflammation.
format Online
Article
Text
id pubmed-9377571
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-93775712022-08-16 Anti-inflammatory effects of neutral lipids, glycolipids, phospholipids from Halocynthia aurantium tunic by suppressing the activation of NF-κB and MAPKs in LPS-stimulated RAW264.7 macrophages Jang, A-yeong Rod-in, Weerawan Monmai, Chaiwat Choi, Gyoung Su Park, Woo Jung PLoS One Research Article Halocynthia aurantium is a marine organism that has been considered a promising source for bio-functional materials. Total lipids were extracted from H. aurantium tunic, and then they were separated into neutral lipids, glycolipids, and phospholipids. In the present study, fatty acid profiles of three lipids and their anti-inflammatory effects in RAW264.7 cells were investigated. Among the lipid classes, phospholipids showed the diversity of fatty acid constituents, compared with the glycolipids and neutral lipids. Three lipids contain different contents of fatty acids depending on the kinds of lipids. The most contents were saturated fatty acids (SFAs, 53–69% of the fatty acids) and monounsaturated fatty acids (MUFAs, 15–17% of fatty acids) and polyunsaturated fatty acids (PUFAs, 14–32% of fatty acids) are followed. H. aurantium lipids not only dose-dependently inhibited nitric oxide production but also reduced the expression of inflammatory cytokine genes such as TNF-α, IL-1β, and IL-6 in LPS-stimulated macrophages. It was also demonstrated that the expression of COX-2 was dose-dependently suppressed. Moreover, H. aurantium lipids decreased phosphorylation of NF-κB p-65, p38, ERK1/2, and JNK, suggesting that three lipids from H. aurantium tunic provide anti-inflammatory effects through NF-κB and MAPK signaling. These results indicate that H. aurantium is a potential source for anti-inflammation. Public Library of Science 2022-08-15 /pmc/articles/PMC9377571/ /pubmed/35969529 http://dx.doi.org/10.1371/journal.pone.0270794 Text en © 2022 Jang et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Jang, A-yeong
Rod-in, Weerawan
Monmai, Chaiwat
Choi, Gyoung Su
Park, Woo Jung
Anti-inflammatory effects of neutral lipids, glycolipids, phospholipids from Halocynthia aurantium tunic by suppressing the activation of NF-κB and MAPKs in LPS-stimulated RAW264.7 macrophages
title Anti-inflammatory effects of neutral lipids, glycolipids, phospholipids from Halocynthia aurantium tunic by suppressing the activation of NF-κB and MAPKs in LPS-stimulated RAW264.7 macrophages
title_full Anti-inflammatory effects of neutral lipids, glycolipids, phospholipids from Halocynthia aurantium tunic by suppressing the activation of NF-κB and MAPKs in LPS-stimulated RAW264.7 macrophages
title_fullStr Anti-inflammatory effects of neutral lipids, glycolipids, phospholipids from Halocynthia aurantium tunic by suppressing the activation of NF-κB and MAPKs in LPS-stimulated RAW264.7 macrophages
title_full_unstemmed Anti-inflammatory effects of neutral lipids, glycolipids, phospholipids from Halocynthia aurantium tunic by suppressing the activation of NF-κB and MAPKs in LPS-stimulated RAW264.7 macrophages
title_short Anti-inflammatory effects of neutral lipids, glycolipids, phospholipids from Halocynthia aurantium tunic by suppressing the activation of NF-κB and MAPKs in LPS-stimulated RAW264.7 macrophages
title_sort anti-inflammatory effects of neutral lipids, glycolipids, phospholipids from halocynthia aurantium tunic by suppressing the activation of nf-κb and mapks in lps-stimulated raw264.7 macrophages
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9377571/
https://www.ncbi.nlm.nih.gov/pubmed/35969529
http://dx.doi.org/10.1371/journal.pone.0270794
work_keys_str_mv AT jangayeong antiinflammatoryeffectsofneutrallipidsglycolipidsphospholipidsfromhalocynthiaaurantiumtunicbysuppressingtheactivationofnfkbandmapksinlpsstimulatedraw2647macrophages
AT rodinweerawan antiinflammatoryeffectsofneutrallipidsglycolipidsphospholipidsfromhalocynthiaaurantiumtunicbysuppressingtheactivationofnfkbandmapksinlpsstimulatedraw2647macrophages
AT monmaichaiwat antiinflammatoryeffectsofneutrallipidsglycolipidsphospholipidsfromhalocynthiaaurantiumtunicbysuppressingtheactivationofnfkbandmapksinlpsstimulatedraw2647macrophages
AT choigyoungsu antiinflammatoryeffectsofneutrallipidsglycolipidsphospholipidsfromhalocynthiaaurantiumtunicbysuppressingtheactivationofnfkbandmapksinlpsstimulatedraw2647macrophages
AT parkwoojung antiinflammatoryeffectsofneutrallipidsglycolipidsphospholipidsfromhalocynthiaaurantiumtunicbysuppressingtheactivationofnfkbandmapksinlpsstimulatedraw2647macrophages