Cargando…

Antibiotic resistance genes of public health importance in livestock and humans in an informal urban community in Nepal

Efforts to mitigate the increasing emergence of antimicrobial resistance (AMR) will benefit from a One Health perspective, as over half of animal antimicrobials are also considered medically important in humans, and AMR can be maintained in the environment. This is especially pertinent to low- and m...

Descripción completa

Detalles Bibliográficos
Autores principales: Young, Cristin C. W., Karmacharya, Dibesh, Bista, Manisha, Sharma, Ajay N., Goldstein, Tracey, Mazet, Jonna A. K., Johnson, Christine K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9378709/
https://www.ncbi.nlm.nih.gov/pubmed/35970981
http://dx.doi.org/10.1038/s41598-022-14781-y
_version_ 1784768574371397632
author Young, Cristin C. W.
Karmacharya, Dibesh
Bista, Manisha
Sharma, Ajay N.
Goldstein, Tracey
Mazet, Jonna A. K.
Johnson, Christine K.
author_facet Young, Cristin C. W.
Karmacharya, Dibesh
Bista, Manisha
Sharma, Ajay N.
Goldstein, Tracey
Mazet, Jonna A. K.
Johnson, Christine K.
author_sort Young, Cristin C. W.
collection PubMed
description Efforts to mitigate the increasing emergence of antimicrobial resistance (AMR) will benefit from a One Health perspective, as over half of animal antimicrobials are also considered medically important in humans, and AMR can be maintained in the environment. This is especially pertinent to low- and middle-income countries and in community settings, where an estimated 80% of all antibiotics are used. This study features AMR genes found among humans, animals, and water at an urban informal settlement in Nepal with intensifying livestock production. We sampled humans, chickens, ducks, swine, and water clustered by household, as well as rodents and shrews near dwellings, concurrently in time in July 2017 in southeastern Kathmandu along the Manohara river. Real-time qualitative PCR was performed to screen for 88 genes. Our results characterize the animal-human-environmental interfaces related to the occurrence of specific resistance genes (bla(SHV-1) (SHV(238G240E) strain), QnrS, ermC, tetA, tetB, aacC2, aadA1) associated with antibiotics of global health importance that comprise several drug classes, including aminoglycosides, beta-lactams, tetracyclines, macrolides, and fluoroquinolones. By characterizing risk factors across AMR genes of public health importance, this research highlights potential transmission pathways for further investigation and provides prioritization of community-based prevention and intervention efforts for disrupting AMR transmission of critically important antibiotics used in both humans and animals in Nepal.
format Online
Article
Text
id pubmed-9378709
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-93787092022-08-17 Antibiotic resistance genes of public health importance in livestock and humans in an informal urban community in Nepal Young, Cristin C. W. Karmacharya, Dibesh Bista, Manisha Sharma, Ajay N. Goldstein, Tracey Mazet, Jonna A. K. Johnson, Christine K. Sci Rep Article Efforts to mitigate the increasing emergence of antimicrobial resistance (AMR) will benefit from a One Health perspective, as over half of animal antimicrobials are also considered medically important in humans, and AMR can be maintained in the environment. This is especially pertinent to low- and middle-income countries and in community settings, where an estimated 80% of all antibiotics are used. This study features AMR genes found among humans, animals, and water at an urban informal settlement in Nepal with intensifying livestock production. We sampled humans, chickens, ducks, swine, and water clustered by household, as well as rodents and shrews near dwellings, concurrently in time in July 2017 in southeastern Kathmandu along the Manohara river. Real-time qualitative PCR was performed to screen for 88 genes. Our results characterize the animal-human-environmental interfaces related to the occurrence of specific resistance genes (bla(SHV-1) (SHV(238G240E) strain), QnrS, ermC, tetA, tetB, aacC2, aadA1) associated with antibiotics of global health importance that comprise several drug classes, including aminoglycosides, beta-lactams, tetracyclines, macrolides, and fluoroquinolones. By characterizing risk factors across AMR genes of public health importance, this research highlights potential transmission pathways for further investigation and provides prioritization of community-based prevention and intervention efforts for disrupting AMR transmission of critically important antibiotics used in both humans and animals in Nepal. Nature Publishing Group UK 2022-08-15 /pmc/articles/PMC9378709/ /pubmed/35970981 http://dx.doi.org/10.1038/s41598-022-14781-y Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Young, Cristin C. W.
Karmacharya, Dibesh
Bista, Manisha
Sharma, Ajay N.
Goldstein, Tracey
Mazet, Jonna A. K.
Johnson, Christine K.
Antibiotic resistance genes of public health importance in livestock and humans in an informal urban community in Nepal
title Antibiotic resistance genes of public health importance in livestock and humans in an informal urban community in Nepal
title_full Antibiotic resistance genes of public health importance in livestock and humans in an informal urban community in Nepal
title_fullStr Antibiotic resistance genes of public health importance in livestock and humans in an informal urban community in Nepal
title_full_unstemmed Antibiotic resistance genes of public health importance in livestock and humans in an informal urban community in Nepal
title_short Antibiotic resistance genes of public health importance in livestock and humans in an informal urban community in Nepal
title_sort antibiotic resistance genes of public health importance in livestock and humans in an informal urban community in nepal
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9378709/
https://www.ncbi.nlm.nih.gov/pubmed/35970981
http://dx.doi.org/10.1038/s41598-022-14781-y
work_keys_str_mv AT youngcristincw antibioticresistancegenesofpublichealthimportanceinlivestockandhumansinaninformalurbancommunityinnepal
AT karmacharyadibesh antibioticresistancegenesofpublichealthimportanceinlivestockandhumansinaninformalurbancommunityinnepal
AT bistamanisha antibioticresistancegenesofpublichealthimportanceinlivestockandhumansinaninformalurbancommunityinnepal
AT sharmaajayn antibioticresistancegenesofpublichealthimportanceinlivestockandhumansinaninformalurbancommunityinnepal
AT goldsteintracey antibioticresistancegenesofpublichealthimportanceinlivestockandhumansinaninformalurbancommunityinnepal
AT mazetjonnaak antibioticresistancegenesofpublichealthimportanceinlivestockandhumansinaninformalurbancommunityinnepal
AT johnsonchristinek antibioticresistancegenesofpublichealthimportanceinlivestockandhumansinaninformalurbancommunityinnepal