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Antibiotic resistance genes of public health importance in livestock and humans in an informal urban community in Nepal
Efforts to mitigate the increasing emergence of antimicrobial resistance (AMR) will benefit from a One Health perspective, as over half of animal antimicrobials are also considered medically important in humans, and AMR can be maintained in the environment. This is especially pertinent to low- and m...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9378709/ https://www.ncbi.nlm.nih.gov/pubmed/35970981 http://dx.doi.org/10.1038/s41598-022-14781-y |
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author | Young, Cristin C. W. Karmacharya, Dibesh Bista, Manisha Sharma, Ajay N. Goldstein, Tracey Mazet, Jonna A. K. Johnson, Christine K. |
author_facet | Young, Cristin C. W. Karmacharya, Dibesh Bista, Manisha Sharma, Ajay N. Goldstein, Tracey Mazet, Jonna A. K. Johnson, Christine K. |
author_sort | Young, Cristin C. W. |
collection | PubMed |
description | Efforts to mitigate the increasing emergence of antimicrobial resistance (AMR) will benefit from a One Health perspective, as over half of animal antimicrobials are also considered medically important in humans, and AMR can be maintained in the environment. This is especially pertinent to low- and middle-income countries and in community settings, where an estimated 80% of all antibiotics are used. This study features AMR genes found among humans, animals, and water at an urban informal settlement in Nepal with intensifying livestock production. We sampled humans, chickens, ducks, swine, and water clustered by household, as well as rodents and shrews near dwellings, concurrently in time in July 2017 in southeastern Kathmandu along the Manohara river. Real-time qualitative PCR was performed to screen for 88 genes. Our results characterize the animal-human-environmental interfaces related to the occurrence of specific resistance genes (bla(SHV-1) (SHV(238G240E) strain), QnrS, ermC, tetA, tetB, aacC2, aadA1) associated with antibiotics of global health importance that comprise several drug classes, including aminoglycosides, beta-lactams, tetracyclines, macrolides, and fluoroquinolones. By characterizing risk factors across AMR genes of public health importance, this research highlights potential transmission pathways for further investigation and provides prioritization of community-based prevention and intervention efforts for disrupting AMR transmission of critically important antibiotics used in both humans and animals in Nepal. |
format | Online Article Text |
id | pubmed-9378709 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-93787092022-08-17 Antibiotic resistance genes of public health importance in livestock and humans in an informal urban community in Nepal Young, Cristin C. W. Karmacharya, Dibesh Bista, Manisha Sharma, Ajay N. Goldstein, Tracey Mazet, Jonna A. K. Johnson, Christine K. Sci Rep Article Efforts to mitigate the increasing emergence of antimicrobial resistance (AMR) will benefit from a One Health perspective, as over half of animal antimicrobials are also considered medically important in humans, and AMR can be maintained in the environment. This is especially pertinent to low- and middle-income countries and in community settings, where an estimated 80% of all antibiotics are used. This study features AMR genes found among humans, animals, and water at an urban informal settlement in Nepal with intensifying livestock production. We sampled humans, chickens, ducks, swine, and water clustered by household, as well as rodents and shrews near dwellings, concurrently in time in July 2017 in southeastern Kathmandu along the Manohara river. Real-time qualitative PCR was performed to screen for 88 genes. Our results characterize the animal-human-environmental interfaces related to the occurrence of specific resistance genes (bla(SHV-1) (SHV(238G240E) strain), QnrS, ermC, tetA, tetB, aacC2, aadA1) associated with antibiotics of global health importance that comprise several drug classes, including aminoglycosides, beta-lactams, tetracyclines, macrolides, and fluoroquinolones. By characterizing risk factors across AMR genes of public health importance, this research highlights potential transmission pathways for further investigation and provides prioritization of community-based prevention and intervention efforts for disrupting AMR transmission of critically important antibiotics used in both humans and animals in Nepal. Nature Publishing Group UK 2022-08-15 /pmc/articles/PMC9378709/ /pubmed/35970981 http://dx.doi.org/10.1038/s41598-022-14781-y Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Young, Cristin C. W. Karmacharya, Dibesh Bista, Manisha Sharma, Ajay N. Goldstein, Tracey Mazet, Jonna A. K. Johnson, Christine K. Antibiotic resistance genes of public health importance in livestock and humans in an informal urban community in Nepal |
title | Antibiotic resistance genes of public health importance in livestock and humans in an informal urban community in Nepal |
title_full | Antibiotic resistance genes of public health importance in livestock and humans in an informal urban community in Nepal |
title_fullStr | Antibiotic resistance genes of public health importance in livestock and humans in an informal urban community in Nepal |
title_full_unstemmed | Antibiotic resistance genes of public health importance in livestock and humans in an informal urban community in Nepal |
title_short | Antibiotic resistance genes of public health importance in livestock and humans in an informal urban community in Nepal |
title_sort | antibiotic resistance genes of public health importance in livestock and humans in an informal urban community in nepal |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9378709/ https://www.ncbi.nlm.nih.gov/pubmed/35970981 http://dx.doi.org/10.1038/s41598-022-14781-y |
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