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Establishment of a system evaluating the contractile force of electrically stimulated myotubes from wrinkles formed on elastic substrate

Muscle weakness is detrimental not only to quality of life but also life expectancy. However, effective drugs have still not been developed to improve and prevent muscle weakness associated with aging or diseases. One reason for the delay in drug discovery is that no suitable in vitro screening syst...

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Autores principales: Hamaguchi, Hiroki, Matsui, Tsubasa S., Deguchi, Shinji, Furuichi, Yasuro, Fujii, Nobuharu L., Manabe, Yasuko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9378739/
https://www.ncbi.nlm.nih.gov/pubmed/35970858
http://dx.doi.org/10.1038/s41598-022-17548-7
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author Hamaguchi, Hiroki
Matsui, Tsubasa S.
Deguchi, Shinji
Furuichi, Yasuro
Fujii, Nobuharu L.
Manabe, Yasuko
author_facet Hamaguchi, Hiroki
Matsui, Tsubasa S.
Deguchi, Shinji
Furuichi, Yasuro
Fujii, Nobuharu L.
Manabe, Yasuko
author_sort Hamaguchi, Hiroki
collection PubMed
description Muscle weakness is detrimental not only to quality of life but also life expectancy. However, effective drugs have still not been developed to improve and prevent muscle weakness associated with aging or diseases. One reason for the delay in drug discovery is that no suitable in vitro screening system has been established to test whether drugs improve muscle strength. Here, we used a specific deformable silicone gel substrate to effectively and sensitively evaluate the contractile force generated by myotubes from wrinkles formed on the substrate. Using this system, it was found that the contractile force generated by an atrophic phenotype of myotubes induced by dexamethasone or cancer cell-conditioned medium treatment significantly decreased while that generated by hypertrophic myotubes induced by insulin-like growth factor-1 significantly increased. Notably, it was found that changes in the index related to contractile force can detect atrophic or hypertrophic phenotypes more sensitively than changes in myotube diameter or myosin heavy chain expression, both commonly used to evaluate myotube function. These results suggest that our proposed system will be an effective tool for assessing the contractile force-related state of myotubes, which are available for the development of drugs to prevent and/or treat muscle weakness.
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spelling pubmed-93787392022-08-17 Establishment of a system evaluating the contractile force of electrically stimulated myotubes from wrinkles formed on elastic substrate Hamaguchi, Hiroki Matsui, Tsubasa S. Deguchi, Shinji Furuichi, Yasuro Fujii, Nobuharu L. Manabe, Yasuko Sci Rep Article Muscle weakness is detrimental not only to quality of life but also life expectancy. However, effective drugs have still not been developed to improve and prevent muscle weakness associated with aging or diseases. One reason for the delay in drug discovery is that no suitable in vitro screening system has been established to test whether drugs improve muscle strength. Here, we used a specific deformable silicone gel substrate to effectively and sensitively evaluate the contractile force generated by myotubes from wrinkles formed on the substrate. Using this system, it was found that the contractile force generated by an atrophic phenotype of myotubes induced by dexamethasone or cancer cell-conditioned medium treatment significantly decreased while that generated by hypertrophic myotubes induced by insulin-like growth factor-1 significantly increased. Notably, it was found that changes in the index related to contractile force can detect atrophic or hypertrophic phenotypes more sensitively than changes in myotube diameter or myosin heavy chain expression, both commonly used to evaluate myotube function. These results suggest that our proposed system will be an effective tool for assessing the contractile force-related state of myotubes, which are available for the development of drugs to prevent and/or treat muscle weakness. Nature Publishing Group UK 2022-08-15 /pmc/articles/PMC9378739/ /pubmed/35970858 http://dx.doi.org/10.1038/s41598-022-17548-7 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Hamaguchi, Hiroki
Matsui, Tsubasa S.
Deguchi, Shinji
Furuichi, Yasuro
Fujii, Nobuharu L.
Manabe, Yasuko
Establishment of a system evaluating the contractile force of electrically stimulated myotubes from wrinkles formed on elastic substrate
title Establishment of a system evaluating the contractile force of electrically stimulated myotubes from wrinkles formed on elastic substrate
title_full Establishment of a system evaluating the contractile force of electrically stimulated myotubes from wrinkles formed on elastic substrate
title_fullStr Establishment of a system evaluating the contractile force of electrically stimulated myotubes from wrinkles formed on elastic substrate
title_full_unstemmed Establishment of a system evaluating the contractile force of electrically stimulated myotubes from wrinkles formed on elastic substrate
title_short Establishment of a system evaluating the contractile force of electrically stimulated myotubes from wrinkles formed on elastic substrate
title_sort establishment of a system evaluating the contractile force of electrically stimulated myotubes from wrinkles formed on elastic substrate
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9378739/
https://www.ncbi.nlm.nih.gov/pubmed/35970858
http://dx.doi.org/10.1038/s41598-022-17548-7
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