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Gut microbiota in systemic lupus erythematosus patients and lupus mouse model: a cross species comparative analysis for biomarker discovery

An increasing number of studies have provided strong evidence that gut microbiota interact with the immune system and stimulate various mechanisms involved in the pathogenesis of auto-immune diseases such as Systemic Lupus Erythematosus (SLE). Indeed, gut microbiota could be a source of diagnostic a...

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Autores principales: Toumi, Eya, Goutorbe, Benoit, Plauzolles, Anne, Bonnet, Marion, Mezouar, Soraya, Militello, Muriel, Mege, Jean-Louis, Chiche, Laurent, Halfon, Philippe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9378784/
https://www.ncbi.nlm.nih.gov/pubmed/35983031
http://dx.doi.org/10.3389/fimmu.2022.943241
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author Toumi, Eya
Goutorbe, Benoit
Plauzolles, Anne
Bonnet, Marion
Mezouar, Soraya
Militello, Muriel
Mege, Jean-Louis
Chiche, Laurent
Halfon, Philippe
author_facet Toumi, Eya
Goutorbe, Benoit
Plauzolles, Anne
Bonnet, Marion
Mezouar, Soraya
Militello, Muriel
Mege, Jean-Louis
Chiche, Laurent
Halfon, Philippe
author_sort Toumi, Eya
collection PubMed
description An increasing number of studies have provided strong evidence that gut microbiota interact with the immune system and stimulate various mechanisms involved in the pathogenesis of auto-immune diseases such as Systemic Lupus Erythematosus (SLE). Indeed, gut microbiota could be a source of diagnostic and prognostic biomarkers but also hold the promise to discover novel therapeutic strategies. Thus far, specific SLE microbial signatures have not yet been clearly identified with alteration patterns that may vary between human and animal studies. In this study, a comparative analysis of a clinically well-characterized cohort of adult patients with SLE showed reduced biodiversity, a lower Firmicutes/Bacteroidetes (F/B) ratio, and six differentially abundant taxa compared with healthy controls. An unsupervised clustering of patients with SLE patients identified a subgroup of patients with a stronger alteration of their gut microbiota. Interestingly, this clustering was strongly correlated with the disease activity assessed with the Systemic Lupus Erythematosus Disease Activity Index (SLEDAI) score (p = 0.03, odd ratio = 15) and the identification of specific alterations involving the F/B ratio and some different taxa. Then, the gut microbiota of pristane-induced lupus and control mice were analyzed for comparison with our human data. Among the six differentially abundant taxa of the human disease signature, five were common with our murine model. Finally, an exhaustive cross-species comparison between our data and previous human and murine SLE studies revealed a core-set of gut microbiome species that might constitute biomarker panels relevant for future validation studies.
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spelling pubmed-93787842022-08-17 Gut microbiota in systemic lupus erythematosus patients and lupus mouse model: a cross species comparative analysis for biomarker discovery Toumi, Eya Goutorbe, Benoit Plauzolles, Anne Bonnet, Marion Mezouar, Soraya Militello, Muriel Mege, Jean-Louis Chiche, Laurent Halfon, Philippe Front Immunol Immunology An increasing number of studies have provided strong evidence that gut microbiota interact with the immune system and stimulate various mechanisms involved in the pathogenesis of auto-immune diseases such as Systemic Lupus Erythematosus (SLE). Indeed, gut microbiota could be a source of diagnostic and prognostic biomarkers but also hold the promise to discover novel therapeutic strategies. Thus far, specific SLE microbial signatures have not yet been clearly identified with alteration patterns that may vary between human and animal studies. In this study, a comparative analysis of a clinically well-characterized cohort of adult patients with SLE showed reduced biodiversity, a lower Firmicutes/Bacteroidetes (F/B) ratio, and six differentially abundant taxa compared with healthy controls. An unsupervised clustering of patients with SLE patients identified a subgroup of patients with a stronger alteration of their gut microbiota. Interestingly, this clustering was strongly correlated with the disease activity assessed with the Systemic Lupus Erythematosus Disease Activity Index (SLEDAI) score (p = 0.03, odd ratio = 15) and the identification of specific alterations involving the F/B ratio and some different taxa. Then, the gut microbiota of pristane-induced lupus and control mice were analyzed for comparison with our human data. Among the six differentially abundant taxa of the human disease signature, five were common with our murine model. Finally, an exhaustive cross-species comparison between our data and previous human and murine SLE studies revealed a core-set of gut microbiome species that might constitute biomarker panels relevant for future validation studies. Frontiers Media S.A. 2022-08-02 /pmc/articles/PMC9378784/ /pubmed/35983031 http://dx.doi.org/10.3389/fimmu.2022.943241 Text en Copyright © 2022 Toumi, Goutorbe, Plauzolles, Bonnet, Mezouar, Militello, Mege, Chiche and Halfon https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Toumi, Eya
Goutorbe, Benoit
Plauzolles, Anne
Bonnet, Marion
Mezouar, Soraya
Militello, Muriel
Mege, Jean-Louis
Chiche, Laurent
Halfon, Philippe
Gut microbiota in systemic lupus erythematosus patients and lupus mouse model: a cross species comparative analysis for biomarker discovery
title Gut microbiota in systemic lupus erythematosus patients and lupus mouse model: a cross species comparative analysis for biomarker discovery
title_full Gut microbiota in systemic lupus erythematosus patients and lupus mouse model: a cross species comparative analysis for biomarker discovery
title_fullStr Gut microbiota in systemic lupus erythematosus patients and lupus mouse model: a cross species comparative analysis for biomarker discovery
title_full_unstemmed Gut microbiota in systemic lupus erythematosus patients and lupus mouse model: a cross species comparative analysis for biomarker discovery
title_short Gut microbiota in systemic lupus erythematosus patients and lupus mouse model: a cross species comparative analysis for biomarker discovery
title_sort gut microbiota in systemic lupus erythematosus patients and lupus mouse model: a cross species comparative analysis for biomarker discovery
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9378784/
https://www.ncbi.nlm.nih.gov/pubmed/35983031
http://dx.doi.org/10.3389/fimmu.2022.943241
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