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An approach to uncover the relationship between 17b-estradiol and ESR1/ESR2 ratio in the regulation of canine corpus luteum
The canine corpus luteum (CL) is able to synthetise, activate and deactivate 17b-estradiol (E2) and also expresses nuclear estrogen receptors in a time-dependent manner during diestrus. Nevertheless, we are still missing a better comprehension of E2 functions in the canine CL, especially regarding t...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9378837/ https://www.ncbi.nlm.nih.gov/pubmed/35982918 http://dx.doi.org/10.3389/fvets.2022.885257 |
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author | Bonfim Neto, Antenor Pereira Cardoso, Ana Paula Mattoso Miskulin Silva, Renata dos Santos Sousa, Liza Margareth Medeiros de Carvalho Giometti, Ines Cristina Binelli, Mario Bauersachs, Stefan Kowalewski, Mariusz Pawel Papa, Paula de Carvalho |
author_facet | Bonfim Neto, Antenor Pereira Cardoso, Ana Paula Mattoso Miskulin Silva, Renata dos Santos Sousa, Liza Margareth Medeiros de Carvalho Giometti, Ines Cristina Binelli, Mario Bauersachs, Stefan Kowalewski, Mariusz Pawel Papa, Paula de Carvalho |
author_sort | Bonfim Neto, Antenor Pereira |
collection | PubMed |
description | The canine corpus luteum (CL) is able to synthetise, activate and deactivate 17b-estradiol (E2) and also expresses nuclear estrogen receptors in a time-dependent manner during diestrus. Nevertheless, we are still missing a better comprehension of E2 functions in the canine CL, especially regarding the specific roles of estrogen receptor alpha (ERa) and ERb, encoded by ESR1 and 2, respectively. For that purpose, we analyzed transcriptomic data of canine non-pregnant CL collected on days 10, 20, 30, 40, 50 and 60 of diestrus and searched for differentially expressed genes (DEG) containing predicted transcription factor binding sites (TFBS) for ESR1 or ESR2. Based on biological functions of DEG presenting TFBS, expression of select transcripts and corresponding proteins was assessed. Additionally, luteal cells were collected across specific time points during diestrus and specificity of E2 responses was tested using ERa and/or ERb inhibitors. Bioinformatic analyses revealed 517 DEGs containing TFBS, from which 67 for both receptors. In general, abundance of predicted ESR1 targets was greater in the beginning, while abundance of ESR2 targets was greater in the end of diestrus. ESR1/ESR2 ratio shifted from an increasing to a decreasing pattern from day 30 to 40 post ovulation. Specific receptor inhibition suggested an ERa-mediated positive regulation of CL function at the beginning of diestrus and an ERb-mediated effect contributing to luteal regression. In conclusion, our data points toward a broad spectrum of action of E2 and its nuclear receptors, which can also act as transcription factors for other genes regulating canine CL function. |
format | Online Article Text |
id | pubmed-9378837 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-93788372022-08-17 An approach to uncover the relationship between 17b-estradiol and ESR1/ESR2 ratio in the regulation of canine corpus luteum Bonfim Neto, Antenor Pereira Cardoso, Ana Paula Mattoso Miskulin Silva, Renata dos Santos Sousa, Liza Margareth Medeiros de Carvalho Giometti, Ines Cristina Binelli, Mario Bauersachs, Stefan Kowalewski, Mariusz Pawel Papa, Paula de Carvalho Front Vet Sci Veterinary Science The canine corpus luteum (CL) is able to synthetise, activate and deactivate 17b-estradiol (E2) and also expresses nuclear estrogen receptors in a time-dependent manner during diestrus. Nevertheless, we are still missing a better comprehension of E2 functions in the canine CL, especially regarding the specific roles of estrogen receptor alpha (ERa) and ERb, encoded by ESR1 and 2, respectively. For that purpose, we analyzed transcriptomic data of canine non-pregnant CL collected on days 10, 20, 30, 40, 50 and 60 of diestrus and searched for differentially expressed genes (DEG) containing predicted transcription factor binding sites (TFBS) for ESR1 or ESR2. Based on biological functions of DEG presenting TFBS, expression of select transcripts and corresponding proteins was assessed. Additionally, luteal cells were collected across specific time points during diestrus and specificity of E2 responses was tested using ERa and/or ERb inhibitors. Bioinformatic analyses revealed 517 DEGs containing TFBS, from which 67 for both receptors. In general, abundance of predicted ESR1 targets was greater in the beginning, while abundance of ESR2 targets was greater in the end of diestrus. ESR1/ESR2 ratio shifted from an increasing to a decreasing pattern from day 30 to 40 post ovulation. Specific receptor inhibition suggested an ERa-mediated positive regulation of CL function at the beginning of diestrus and an ERb-mediated effect contributing to luteal regression. In conclusion, our data points toward a broad spectrum of action of E2 and its nuclear receptors, which can also act as transcription factors for other genes regulating canine CL function. Frontiers Media S.A. 2022-08-02 /pmc/articles/PMC9378837/ /pubmed/35982918 http://dx.doi.org/10.3389/fvets.2022.885257 Text en Copyright © 2022 Bonfim Neto, Cardoso, Silva, Sousa, Giometti, Binelli, Bauersachs, Kowalewski and Papa. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Veterinary Science Bonfim Neto, Antenor Pereira Cardoso, Ana Paula Mattoso Miskulin Silva, Renata dos Santos Sousa, Liza Margareth Medeiros de Carvalho Giometti, Ines Cristina Binelli, Mario Bauersachs, Stefan Kowalewski, Mariusz Pawel Papa, Paula de Carvalho An approach to uncover the relationship between 17b-estradiol and ESR1/ESR2 ratio in the regulation of canine corpus luteum |
title | An approach to uncover the relationship between 17b-estradiol and ESR1/ESR2 ratio in the regulation of canine corpus luteum |
title_full | An approach to uncover the relationship between 17b-estradiol and ESR1/ESR2 ratio in the regulation of canine corpus luteum |
title_fullStr | An approach to uncover the relationship between 17b-estradiol and ESR1/ESR2 ratio in the regulation of canine corpus luteum |
title_full_unstemmed | An approach to uncover the relationship between 17b-estradiol and ESR1/ESR2 ratio in the regulation of canine corpus luteum |
title_short | An approach to uncover the relationship between 17b-estradiol and ESR1/ESR2 ratio in the regulation of canine corpus luteum |
title_sort | approach to uncover the relationship between 17b-estradiol and esr1/esr2 ratio in the regulation of canine corpus luteum |
topic | Veterinary Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9378837/ https://www.ncbi.nlm.nih.gov/pubmed/35982918 http://dx.doi.org/10.3389/fvets.2022.885257 |
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