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Decrease of circARID1A retards glioblastoma invasion by modulating miR-370-3p/ TGFBR2 pathway

Increasing evidence suggests that circular RNAs (circRNAs) are involved in regulating tumor biological activity. Glioblastoma (GBM) is one of the most lethal diseases characterized by highly aggressive proliferative and invasive behaviors. We aimed to explore how circRNAs influenced GBM biological a...

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Autores principales: Li, Baisheng, Chen, Jiansheng, Wu, Yi, Luo, Honghai, Ke, Yiquan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9379412/
https://www.ncbi.nlm.nih.gov/pubmed/35982888
http://dx.doi.org/10.7150/ijbs.66673
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author Li, Baisheng
Chen, Jiansheng
Wu, Yi
Luo, Honghai
Ke, Yiquan
author_facet Li, Baisheng
Chen, Jiansheng
Wu, Yi
Luo, Honghai
Ke, Yiquan
author_sort Li, Baisheng
collection PubMed
description Increasing evidence suggests that circular RNAs (circRNAs) are involved in regulating tumor biological activity. Glioblastoma (GBM) is one of the most lethal diseases characterized by highly aggressive proliferative and invasive behaviors. We aimed to explore how circRNAs influenced GBM biological activity. By circRNA array analysis we found that circARID1A was significantly up-regulated in GBM. Next, we found that circARID1A was up-regulated in GBM tissues and cell lines. Interfering with circARID1A inhibited the migration and invasion of a human GBM cell line U87. By performing dual-luciferase reporter assays, RNA pull-down and fluorescent in situ hybridization (FISH), we determined that circARID1A directly bound to miR-370-3p. Moreover, we confirmed that transforming growth factor beta receptor 2 (TGFBR2) was the target gene of miR-370-3p by performing RNA pull-down, dual-luciferase reporter assays and western blotting. Further experiments verified that circARID1A promoted GBM cell migration and invasion by modulating miR-370-3p/ TGFBR2 pathway. In addition, we demonstrated that silencing circARID1A restrain the growth of GBM in vivo. Finally, we showed that circARID1A was abundant in GBM cell derived exosomes. In conclusion, circARID1A participated in regulating migration and invasion of GBM via modulation of miR-370-3p/ TGFBR2 and thus may be a potential serum biomarker of GBM.
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spelling pubmed-93794122022-08-17 Decrease of circARID1A retards glioblastoma invasion by modulating miR-370-3p/ TGFBR2 pathway Li, Baisheng Chen, Jiansheng Wu, Yi Luo, Honghai Ke, Yiquan Int J Biol Sci Research Paper Increasing evidence suggests that circular RNAs (circRNAs) are involved in regulating tumor biological activity. Glioblastoma (GBM) is one of the most lethal diseases characterized by highly aggressive proliferative and invasive behaviors. We aimed to explore how circRNAs influenced GBM biological activity. By circRNA array analysis we found that circARID1A was significantly up-regulated in GBM. Next, we found that circARID1A was up-regulated in GBM tissues and cell lines. Interfering with circARID1A inhibited the migration and invasion of a human GBM cell line U87. By performing dual-luciferase reporter assays, RNA pull-down and fluorescent in situ hybridization (FISH), we determined that circARID1A directly bound to miR-370-3p. Moreover, we confirmed that transforming growth factor beta receptor 2 (TGFBR2) was the target gene of miR-370-3p by performing RNA pull-down, dual-luciferase reporter assays and western blotting. Further experiments verified that circARID1A promoted GBM cell migration and invasion by modulating miR-370-3p/ TGFBR2 pathway. In addition, we demonstrated that silencing circARID1A restrain the growth of GBM in vivo. Finally, we showed that circARID1A was abundant in GBM cell derived exosomes. In conclusion, circARID1A participated in regulating migration and invasion of GBM via modulation of miR-370-3p/ TGFBR2 and thus may be a potential serum biomarker of GBM. Ivyspring International Publisher 2022-08-08 /pmc/articles/PMC9379412/ /pubmed/35982888 http://dx.doi.org/10.7150/ijbs.66673 Text en © The author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Li, Baisheng
Chen, Jiansheng
Wu, Yi
Luo, Honghai
Ke, Yiquan
Decrease of circARID1A retards glioblastoma invasion by modulating miR-370-3p/ TGFBR2 pathway
title Decrease of circARID1A retards glioblastoma invasion by modulating miR-370-3p/ TGFBR2 pathway
title_full Decrease of circARID1A retards glioblastoma invasion by modulating miR-370-3p/ TGFBR2 pathway
title_fullStr Decrease of circARID1A retards glioblastoma invasion by modulating miR-370-3p/ TGFBR2 pathway
title_full_unstemmed Decrease of circARID1A retards glioblastoma invasion by modulating miR-370-3p/ TGFBR2 pathway
title_short Decrease of circARID1A retards glioblastoma invasion by modulating miR-370-3p/ TGFBR2 pathway
title_sort decrease of circarid1a retards glioblastoma invasion by modulating mir-370-3p/ tgfbr2 pathway
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9379412/
https://www.ncbi.nlm.nih.gov/pubmed/35982888
http://dx.doi.org/10.7150/ijbs.66673
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