The Novel Role of hnRNP UL1 in Human Cell Nucleoli

hnRNP UL1 plays an important role in cell nuclei, where it is recruited to DNA damage sites and is involved in the repair of DNA double-strand breaks. Furthermore, this protein is known as a transcriptional repressor of RNA polymerase II genes. In the present study, we have shown that hnRNP UL1 is a...

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Autores principales: Cichocka, Marlena, Karlik, Anna, Plewka, Patrycja, Gawade, Kishor, Stępień, Agata, Świergiel, Patrycja, Gadgil, Ankur, Raczyńska, Katarzyna Dorota
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2022
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9379416/
https://www.ncbi.nlm.nih.gov/pubmed/35982897
http://dx.doi.org/10.7150/ijbs.75084
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author Cichocka, Marlena
Karlik, Anna
Plewka, Patrycja
Gawade, Kishor
Stępień, Agata
Świergiel, Patrycja
Gadgil, Ankur
Raczyńska, Katarzyna Dorota
author_facet Cichocka, Marlena
Karlik, Anna
Plewka, Patrycja
Gawade, Kishor
Stępień, Agata
Świergiel, Patrycja
Gadgil, Ankur
Raczyńska, Katarzyna Dorota
author_sort Cichocka, Marlena
collection PubMed
description hnRNP UL1 plays an important role in cell nuclei, where it is recruited to DNA damage sites and is involved in the repair of DNA double-strand breaks. Furthermore, this protein is known as a transcriptional repressor of RNA polymerase II genes. In the present study, we have shown that hnRNP UL1 is also localized in the nucleoli of human cells. Upon investigating its function, we found that hnRNP UL1 stimulates ribosomal DNA (rDNA) gene transcription. Moreover, we observed that cells with hnRNP UL1 silencing exhibited increased sensitivity to DNA damage. We also showed that hnRNP UL1 interacts with γH2A.X, RPA32, XRCC1, and Chk1 in cell nucleoli, suggesting its involvement in the repair of rDNA damage.
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spelling pubmed-93794162022-08-17 The Novel Role of hnRNP UL1 in Human Cell Nucleoli Cichocka, Marlena Karlik, Anna Plewka, Patrycja Gawade, Kishor Stępień, Agata Świergiel, Patrycja Gadgil, Ankur Raczyńska, Katarzyna Dorota Int J Biol Sci Research Paper hnRNP UL1 plays an important role in cell nuclei, where it is recruited to DNA damage sites and is involved in the repair of DNA double-strand breaks. Furthermore, this protein is known as a transcriptional repressor of RNA polymerase II genes. In the present study, we have shown that hnRNP UL1 is also localized in the nucleoli of human cells. Upon investigating its function, we found that hnRNP UL1 stimulates ribosomal DNA (rDNA) gene transcription. Moreover, we observed that cells with hnRNP UL1 silencing exhibited increased sensitivity to DNA damage. We also showed that hnRNP UL1 interacts with γH2A.X, RPA32, XRCC1, and Chk1 in cell nucleoli, suggesting its involvement in the repair of rDNA damage. Ivyspring International Publisher 2022-07-18 /pmc/articles/PMC9379416/ /pubmed/35982897 http://dx.doi.org/10.7150/ijbs.75084 Text en © The author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Cichocka, Marlena
Karlik, Anna
Plewka, Patrycja
Gawade, Kishor
Stępień, Agata
Świergiel, Patrycja
Gadgil, Ankur
Raczyńska, Katarzyna Dorota
The Novel Role of hnRNP UL1 in Human Cell Nucleoli
title The Novel Role of hnRNP UL1 in Human Cell Nucleoli
title_full The Novel Role of hnRNP UL1 in Human Cell Nucleoli
title_fullStr The Novel Role of hnRNP UL1 in Human Cell Nucleoli
title_full_unstemmed The Novel Role of hnRNP UL1 in Human Cell Nucleoli
title_short The Novel Role of hnRNP UL1 in Human Cell Nucleoli
title_sort novel role of hnrnp ul1 in human cell nucleoli
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9379416/
https://www.ncbi.nlm.nih.gov/pubmed/35982897
http://dx.doi.org/10.7150/ijbs.75084
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