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Construction and Immunogenicity of a Recombinant Pseudorabies Virus Expressing SARS-CoV-2-S and SARS-CoV-2-N

Coronavirus (CoV) is an important pathogen of humans and animals, which can infect humans or animals through the respiratory mucosal route. Syndrome coronavirus 2 (SARS-CoV-2) is quite similar to syndrome coronavirus (SARS-CoV) with the same receptor, angiotensin-converting enzyme 2 (ACE2). The S an...

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Autores principales: Li, Ruoying, Shao, Guanming, Xie, Zi, Hu, Zezhong, Feng, Keyu, He, Jiahui, Wang, Hailong, Fu, Jun, Zhang, Xinheng, Xie, Qingmei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9380597/
https://www.ncbi.nlm.nih.gov/pubmed/35982925
http://dx.doi.org/10.3389/fvets.2022.920087
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author Li, Ruoying
Shao, Guanming
Xie, Zi
Hu, Zezhong
Feng, Keyu
He, Jiahui
Wang, Hailong
Fu, Jun
Zhang, Xinheng
Xie, Qingmei
author_facet Li, Ruoying
Shao, Guanming
Xie, Zi
Hu, Zezhong
Feng, Keyu
He, Jiahui
Wang, Hailong
Fu, Jun
Zhang, Xinheng
Xie, Qingmei
author_sort Li, Ruoying
collection PubMed
description Coronavirus (CoV) is an important pathogen of humans and animals, which can infect humans or animals through the respiratory mucosal route. Syndrome coronavirus 2 (SARS-CoV-2) is quite similar to syndrome coronavirus (SARS-CoV) with the same receptor, angiotensin-converting enzyme 2 (ACE2). The S and N proteins are the most important protective antigens of the SARS-CoV-2. The S protein on the viral membrane mediates the virus attachment with the host cells, and the N protein is the most abundant expression during infection. In this study, the recombinant viruses expressing the S and N proteins of SARS-CoV-2 were successfully constructed by Red/ET recombinant technology using Pseudorabies virus (PRV) strain Bartha-K61 as a vector. Genetic stability and growth kinetics analysis showed that the recombinant viruses rPRV-SARS-CoV-2-S and rPRV-SARS-CoV-2-N had similar genetic stability and proliferation characteristics to the parental PRV. The immunoassay results showed that mice immunized with recombinant viruses could produce total IgG antibodies. Therefore, PRV is feasible and promising as a viral vector to express SARS-CoV-2-S and SARS-CoV-2-N genes. This study can provide a reference for future research on live vector vaccines for domestic animals, pets, and wild animals.
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spelling pubmed-93805972022-08-17 Construction and Immunogenicity of a Recombinant Pseudorabies Virus Expressing SARS-CoV-2-S and SARS-CoV-2-N Li, Ruoying Shao, Guanming Xie, Zi Hu, Zezhong Feng, Keyu He, Jiahui Wang, Hailong Fu, Jun Zhang, Xinheng Xie, Qingmei Front Vet Sci Veterinary Science Coronavirus (CoV) is an important pathogen of humans and animals, which can infect humans or animals through the respiratory mucosal route. Syndrome coronavirus 2 (SARS-CoV-2) is quite similar to syndrome coronavirus (SARS-CoV) with the same receptor, angiotensin-converting enzyme 2 (ACE2). The S and N proteins are the most important protective antigens of the SARS-CoV-2. The S protein on the viral membrane mediates the virus attachment with the host cells, and the N protein is the most abundant expression during infection. In this study, the recombinant viruses expressing the S and N proteins of SARS-CoV-2 were successfully constructed by Red/ET recombinant technology using Pseudorabies virus (PRV) strain Bartha-K61 as a vector. Genetic stability and growth kinetics analysis showed that the recombinant viruses rPRV-SARS-CoV-2-S and rPRV-SARS-CoV-2-N had similar genetic stability and proliferation characteristics to the parental PRV. The immunoassay results showed that mice immunized with recombinant viruses could produce total IgG antibodies. Therefore, PRV is feasible and promising as a viral vector to express SARS-CoV-2-S and SARS-CoV-2-N genes. This study can provide a reference for future research on live vector vaccines for domestic animals, pets, and wild animals. Frontiers Media S.A. 2022-08-02 /pmc/articles/PMC9380597/ /pubmed/35982925 http://dx.doi.org/10.3389/fvets.2022.920087 Text en Copyright © 2022 Li, Shao, Xie, Hu, Feng, He, Wang, Fu, Zhang and Xie. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Li, Ruoying
Shao, Guanming
Xie, Zi
Hu, Zezhong
Feng, Keyu
He, Jiahui
Wang, Hailong
Fu, Jun
Zhang, Xinheng
Xie, Qingmei
Construction and Immunogenicity of a Recombinant Pseudorabies Virus Expressing SARS-CoV-2-S and SARS-CoV-2-N
title Construction and Immunogenicity of a Recombinant Pseudorabies Virus Expressing SARS-CoV-2-S and SARS-CoV-2-N
title_full Construction and Immunogenicity of a Recombinant Pseudorabies Virus Expressing SARS-CoV-2-S and SARS-CoV-2-N
title_fullStr Construction and Immunogenicity of a Recombinant Pseudorabies Virus Expressing SARS-CoV-2-S and SARS-CoV-2-N
title_full_unstemmed Construction and Immunogenicity of a Recombinant Pseudorabies Virus Expressing SARS-CoV-2-S and SARS-CoV-2-N
title_short Construction and Immunogenicity of a Recombinant Pseudorabies Virus Expressing SARS-CoV-2-S and SARS-CoV-2-N
title_sort construction and immunogenicity of a recombinant pseudorabies virus expressing sars-cov-2-s and sars-cov-2-n
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9380597/
https://www.ncbi.nlm.nih.gov/pubmed/35982925
http://dx.doi.org/10.3389/fvets.2022.920087
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